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C-reactive protein (CRP) is used as a general biomarker for inflammation and infection. During stroke and myocardial infarction, CRP increases and is present in a broad concentration range of 1–500 μg/mL. Therefore, full-range CRP detection is crucial to identify patients who need close follow-up or intensive treatment after a heart attack. Here, we report the first attempt to develop an electrochemiluminescent lateral flow immunosensor (ECL-LFI) that allows full-range CRP detection. Ru(bpy)32+-labeled gold nanoparticles (AuNPs) are used as a CRP-targeting probe and a signal generator; they form sandwich immunocomplexes at the test line of the strip and generate strong ECL emission via a Ru(bpy)32+/tripropylamine system. The ECL-LFI shows high sensitivity in detecting CRP in spiked serum, with a limit of detection of 4.6 pg/mL within 15 min, and a broad detection range of 0.01–1000 ng/mL, which is 2 orders of magnitude broader than that of conventional colorimetric LFI. The clinical usability of the ECL-LFI was evaluated using 30 clinical serum samples (200 ng/mL to 5 mg/mL), which showed a good linear correlation (R2 = 0.9896), with a clinical chemistry analyzer. The results suggest that the ECL-LFI holds great potential for CRP detection in point-of-care diagnostics.
Donggu Hong; Kihyeun Kim; Eun-Jung Jo; Min-Gon Kim. Electrochemiluminescence-Incorporated Lateral Flow Immunosensors Using Ru(bpy)32+-Labeled Gold Nanoparticles for the Full-Range Detection of Physiological C-Reactive Protein Levels. Analytical Chemistry 2021, 93, 7925 -7932.
AMA StyleDonggu Hong, Kihyeun Kim, Eun-Jung Jo, Min-Gon Kim. Electrochemiluminescence-Incorporated Lateral Flow Immunosensors Using Ru(bpy)32+-Labeled Gold Nanoparticles for the Full-Range Detection of Physiological C-Reactive Protein Levels. Analytical Chemistry. 2021; 93 (22):7925-7932.
Chicago/Turabian StyleDonggu Hong; Kihyeun Kim; Eun-Jung Jo; Min-Gon Kim. 2021. "Electrochemiluminescence-Incorporated Lateral Flow Immunosensors Using Ru(bpy)32+-Labeled Gold Nanoparticles for the Full-Range Detection of Physiological C-Reactive Protein Levels." Analytical Chemistry 93, no. 22: 7925-7932.
There is a demand for one-pot, portable (solid-phase), sensitive, and user-friendly immunosensors for future point-of-care (POC) self-testing. However, current immunoassays such as the enzyme-linked immunosorbent assay (ELISA) typically involve several complicated steps, and they are not readily adaptable by nonexpert users. Herein, we present a rapid (∼30 min) one-pot, solid-phase immunosensor, based on nanomaterials, by combining a nanometer-thick Au/TiO2 photocatalytic film and Cy5/capture antibody/gold nanorod (GNR) conjugates immobilized on a membrane (the fluorescence of Cy5 was enhanced by the GNR). The one-pot immunoassay is started by adding a drop of a mixture containing 4-chloro-1-naphthol (CN), a horseradish peroxidase (HRP)-labeled detection antibody, and an antigen onto the one-pot immunosensor and illuminating UV light. The UV illumination on the Au/TiO2 film results in the production of H2O2, which promotes a CN precipitation reaction. 4-Chloro-1-naphthol precipitates produced by the HRP, which was bound to the conjugates via the antibodies and antigens, could preliminarily quench Cy5 fluorescence via Förster resonance energy transfer, because of their proximity to Cy5. The sensitivity of the developed one-pot immunosensor was similar to that of a commercially available ELISA kit. Given the increasing interest in the early diagnosis of various diseases, including cancers, dementia, and coronavirus disease 2019, the application of nanomaterials such as a porous thin-film photocatalyst and GNR-based fluorescent probes could pave way for the development of next-generation POC biosensors.
Kihyeun Kim; Eun-Jung Jo; Donggu Hong; Hyun-Kyung Oh; Ki Joong Lee; Yong-Beom Shin; Min-Gon Kim. One-Pot, Solid-Phase Immunosensing Platform Consisting of a Nanometer-Thick Au/TiO2 Photocatalytic Film and Cy5/Capture Antibody/Gold Nanorod Conjugates. ACS Applied Nano Materials 2021, 4, 5454 -5460.
AMA StyleKihyeun Kim, Eun-Jung Jo, Donggu Hong, Hyun-Kyung Oh, Ki Joong Lee, Yong-Beom Shin, Min-Gon Kim. One-Pot, Solid-Phase Immunosensing Platform Consisting of a Nanometer-Thick Au/TiO2 Photocatalytic Film and Cy5/Capture Antibody/Gold Nanorod Conjugates. ACS Applied Nano Materials. 2021; 4 (5):5454-5460.
Chicago/Turabian StyleKihyeun Kim; Eun-Jung Jo; Donggu Hong; Hyun-Kyung Oh; Ki Joong Lee; Yong-Beom Shin; Min-Gon Kim. 2021. "One-Pot, Solid-Phase Immunosensing Platform Consisting of a Nanometer-Thick Au/TiO2 Photocatalytic Film and Cy5/Capture Antibody/Gold Nanorod Conjugates." ACS Applied Nano Materials 4, no. 5: 5454-5460.
Paper-based biosensors based on lateral flow immunoassay (LFI) are promising candidates for POC diagnosis because of their ease of use and rapid target detection. However, the low sensitivity of LFI limits its application, and signal amplification has been used in numerous studies to increase its sensitivity. We developed an advanced trap LFI (α-trapLFI), a simple-to-use sensor, with an additional step for signal amplification. Here, signal amplification is automatically implemented following delayed release of enhancement solution induced by water-soluble polyvinyl alcohol tape. As the polyvinyl alcohol tape is exposed to water, its polymer structure is perturbed (within 5 min), allowing ions to pass through. This new sensor was designed to have a short time delay between the flow of solutions used for the immunoassay and signal amplification. The α-trapLFI was subsequently used to detect cortisol with high sensitivity (9.1 pg∙mL-1) over a broad detection range (0.01–1000 ng∙mL-1) in bodily fluids. Furthermore, an excellent correlation was obtained by analyzing 20 human saliva samples using this sensor and a conventional ELISA (R2 = 0.90). The new sensor will be helpful in detecting various small molecules for simple, rapid, and portable POC diagnosis of stress disorders.
Hyun-Kyung Oh; Kihyeun Kim; Hyungjun Jang; Jinhee Park; Min-Gon Kim. Advanced Trap Lateral Flow Immunoassay Sensor for the Detection of Cortisol in Human Bodily Fluids. 2021, 1 .
AMA StyleHyun-Kyung Oh, Kihyeun Kim, Hyungjun Jang, Jinhee Park, Min-Gon Kim. Advanced Trap Lateral Flow Immunoassay Sensor for the Detection of Cortisol in Human Bodily Fluids. . 2021; ():1.
Chicago/Turabian StyleHyun-Kyung Oh; Kihyeun Kim; Hyungjun Jang; Jinhee Park; Min-Gon Kim. 2021. "Advanced Trap Lateral Flow Immunoassay Sensor for the Detection of Cortisol in Human Bodily Fluids." , no. : 1.
While quinoidal moieties are considered as emerging platforms showing efficient charge transport and interesting open-shell diradical characteristics, whether these properties could be changed by extension to the conjugated polymer structure remains as a fundamental question. Here, we developed and characterized two conjugated polymers incorporating quinoids with different lengths, which have a stable close- and open-shell diradical character, respectively, namely, poly(quinoidal thiophene-thienylene vinylene) (PQuT-TV) and poly(quinoidal bithiophene-thienylene vinylene) (PQuBT-TV). A longer length of a quinoidal core led to enhanced diradical characteristics. Therefore, the longer core length of QuBT was favorable for the formation of an open-shell diradical structure in its monomer and in the quinoidal polymer. PQuBT-TV exhibited high spin characteristics observed by the strong ESR signal, a low band gap, and improved electrochemical stability. On the other hand, as QuT maintained a closed-shell quinoid structure, PQuT-TV exhibited high backbone coplanarity and strong intermolecular interaction, which was beneficial for charge transport and led to high hole mobility (up to 2.40 cm2 V–1 s–1) in organic field-effect transistors. This work successfully demonstrated how the control of the closed/open-shell character of quinoidal building blocks changes charge transport and spin properties of quinoidal conjugated polymers via quinoid–aromatic interconversion.
YunSeul Kim; Yeon-Ju Kim; Yeong-A Kim; Eunhwan Jung; Yoonjung Mok; Kihyeun Kim; Hansu Hwang; Jong-Jin Park; Min-Gon Kim; Sanjay Mathur; Dong-Yu Kim. Open-Shell and Closed-Shell Quinoid–Aromatic Conjugated Polymers: Unusual Spin Magnetic and High Charge Transport Properties. ACS Applied Materials & Interfaces 2021, 13, 2887 -2898.
AMA StyleYunSeul Kim, Yeon-Ju Kim, Yeong-A Kim, Eunhwan Jung, Yoonjung Mok, Kihyeun Kim, Hansu Hwang, Jong-Jin Park, Min-Gon Kim, Sanjay Mathur, Dong-Yu Kim. Open-Shell and Closed-Shell Quinoid–Aromatic Conjugated Polymers: Unusual Spin Magnetic and High Charge Transport Properties. ACS Applied Materials & Interfaces. 2021; 13 (2):2887-2898.
Chicago/Turabian StyleYunSeul Kim; Yeon-Ju Kim; Yeong-A Kim; Eunhwan Jung; Yoonjung Mok; Kihyeun Kim; Hansu Hwang; Jong-Jin Park; Min-Gon Kim; Sanjay Mathur; Dong-Yu Kim. 2021. "Open-Shell and Closed-Shell Quinoid–Aromatic Conjugated Polymers: Unusual Spin Magnetic and High Charge Transport Properties." ACS Applied Materials & Interfaces 13, no. 2: 2887-2898.
Nucleic acids have been widely recognized as ideal biomarkers in various applications, such as microorganism detection, [50] medical diagnostics [15, 24, 25], food safety [9, 54], environmental monitoring [73], and science forensics [53]. In these areas, nucleic acid testing (NAT) is a widely used analytical tool, as the direct detection of nucleic acid is a highly sensitive and specific technology among early diagnostic methods [56]. NAT is also called molecular diagnostics or genetic testing, which refers to the detection of specific DNA or RNA sequences in the target samples. Based on its outstanding sensitivity [30], NAT has become the standard diagnostic method for the detection of harmful microorganisms, [13] representing a significant index recorded in many types of market reports.
Bhagwan S. Batule; Youngung Seok; Min-Gon Kim. Paper-Based Molecular Diagnostics. Biomedical and Resonance Optics 2020, 155 -181.
AMA StyleBhagwan S. Batule, Youngung Seok, Min-Gon Kim. Paper-Based Molecular Diagnostics. Biomedical and Resonance Optics. 2020; ():155-181.
Chicago/Turabian StyleBhagwan S. Batule; Youngung Seok; Min-Gon Kim. 2020. "Paper-Based Molecular Diagnostics." Biomedical and Resonance Optics , no. : 155-181.
The detection of trace protein biomarkers is essential in the diagnostic field. Protein detection systems ranging from widely used enzyme-linked immunosorbent assays to simple, inexpensive approaches, such as lateral flow immunoassays, play critical roles in medical and drug research. Despite continuous progress, current systems are insufficient for the diagnosis of diseases that require high sensitivity. In this study, we developed a heterogeneous sandwich-type sensing platform based on recombinase polymerase amplification using DNA aptamers specific to the target biomarker. Only the DNA bound to the target in the form of a heterogeneous sandwich was selectively amplified, and the fluorescence signal of an intercalating dye added before the amplification reaction was detected, thereby enabling high specificity and sensitivity. We applied this method for the detection of protein biomarkers for various infectious diseases including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and observed attomolar-level detection of biomarkers and low cross-reactivity between different viruses. We also confirmed detection efficiency of the proposed method using clinical samples. These results demonstrate that the proposed sensing platform can be used to diagnose various diseases requiring high sensitivity, specificity, and accuracy.
Juyoung Kang; Hyungjun Jang; Gyuho Yeom; Min-Gon Kim. Ultrasensitive Detection Platform of Disease Biomarkers Based on Recombinase Polymerase Amplification with H-Sandwich Aptamers. Analytical Chemistry 2020, 93, 992 -1000.
AMA StyleJuyoung Kang, Hyungjun Jang, Gyuho Yeom, Min-Gon Kim. Ultrasensitive Detection Platform of Disease Biomarkers Based on Recombinase Polymerase Amplification with H-Sandwich Aptamers. Analytical Chemistry. 2020; 93 (2):992-1000.
Chicago/Turabian StyleJuyoung Kang; Hyungjun Jang; Gyuho Yeom; Min-Gon Kim. 2020. "Ultrasensitive Detection Platform of Disease Biomarkers Based on Recombinase Polymerase Amplification with H-Sandwich Aptamers." Analytical Chemistry 93, no. 2: 992-1000.
The portability of electronic-based biosensors is limited because of the use of batteries and/or solutions containing reactants such as enzymes for assay, which limits the utility of such biosensors in point-of-care (POC) testing. In this study, we report on the development of a self-powered biosensor composed of only portable components: a reactant-containing poly (ethylene glycol) (PEG) film for the colorimetric assay, and a self-powered n-InGaZnO/p-Si photodetector. The PEG film containing enzymes and color-developing agents was formed on a glass slide by spin coating. The self-powered biosensor was fabricated by placing the hybrid film on the p-n junction photodetector, and applied in non-invasive glucose detection (salivary glucose). Injection of the target-containing solution dissolved the PEG that led to the release of enzymes and color-developing agents, resulting in a colorimetric assay. The colorimetric assay could attenuate the light reaching the photodetector, thus facilitating target concentration verification by measuring the photocurrent. Our self-powered biosensor has two main advantages: (i) all components of the biosensor are portable and (ii) dilution of target concentration is avoided as the reagents are in the PEG film. Therefore, the self-powered biosensor, without solution-phase components, could be highly beneficial for creating portable, sensitive biosensors for POC testing.
Kihyeun Kim; Hyeonghun Kim; Eun-Jung Jo; Hyungjun Jang; JiYoon Park; Gun Young Jung; Min-Gon Kim. Reactant/polymer hybrid films on p-n junction photodetectors for self-powered, non-invasive glucose biosensors. Biosensors and Bioelectronics 2020, 175, 112855 .
AMA StyleKihyeun Kim, Hyeonghun Kim, Eun-Jung Jo, Hyungjun Jang, JiYoon Park, Gun Young Jung, Min-Gon Kim. Reactant/polymer hybrid films on p-n junction photodetectors for self-powered, non-invasive glucose biosensors. Biosensors and Bioelectronics. 2020; 175 ():112855.
Chicago/Turabian StyleKihyeun Kim; Hyeonghun Kim; Eun-Jung Jo; Hyungjun Jang; JiYoon Park; Gun Young Jung; Min-Gon Kim. 2020. "Reactant/polymer hybrid films on p-n junction photodetectors for self-powered, non-invasive glucose biosensors." Biosensors and Bioelectronics 175, no. : 112855.
In this study, a signal-amplifiable nanoprobe-based chemiluminescent lateral flow immunoassay (CL-LFA) was developed to detect avian influenza viruses (AIV) and other contagious and fatal viral avian-origin diseases worldwide. Signal-amplifiable nanoprobes are capable of size-selective immobilization of antibodies (binding receptors) and enzymes (signal transducers) on sensitive paper-based sensor platforms. Particle structure designs and conjugation pathways conducive for antigen accessibility to maximum amounts of immobilized enzymes and antibodies have advanced. The detection limit of the CL-LFA using the signal-amplifiable nanoprobe for the nucleoprotein of the H3N2 virus was 5 pM. Sensitivity tests for low pathogenicity avian influenza H9N2, H1N1, and high pathogenicity avian influenza H5N9 viruses were conducted, and the detection limits of CL-LFA were found to be 103.5 50% egg infective dose (EID50)/mL, 102.5 EID50/mL, and 104 EID50/mL, respectively, which is 20 to 100 times lower than that of a commercial AIV rapid test kit. Moreover, CL-LFA demonstrated high sensitivity and specificity against 37 clinical samples. The signal-amplifiable probe designed in this study is a potential diagnostic probe with ultrahigh sensitivity for applications in the field of clinical diagnosis, which requires sensitive antigen detection as evidenced by enhanced signaling capacity and sensitivity of the LFAs.
Huijin Jung; Sung Hyeon Park; Jiho Lee; Byeongdu Lee; Jinyoung Park; Youngung Seok; Jong-Ho Choi; Min-Gon Kim; Chang-Seon Song; Joonseok Lee. A Size-Selectively Biomolecule-Immobilized Nanoprobe-Based Chemiluminescent Lateral Flow Immunoassay for Detection of Avian-Origin Viruses. Analytical Chemistry 2020, 93, 792 -800.
AMA StyleHuijin Jung, Sung Hyeon Park, Jiho Lee, Byeongdu Lee, Jinyoung Park, Youngung Seok, Jong-Ho Choi, Min-Gon Kim, Chang-Seon Song, Joonseok Lee. A Size-Selectively Biomolecule-Immobilized Nanoprobe-Based Chemiluminescent Lateral Flow Immunoassay for Detection of Avian-Origin Viruses. Analytical Chemistry. 2020; 93 (2):792-800.
Chicago/Turabian StyleHuijin Jung; Sung Hyeon Park; Jiho Lee; Byeongdu Lee; Jinyoung Park; Youngung Seok; Jong-Ho Choi; Min-Gon Kim; Chang-Seon Song; Joonseok Lee. 2020. "A Size-Selectively Biomolecule-Immobilized Nanoprobe-Based Chemiluminescent Lateral Flow Immunoassay for Detection of Avian-Origin Viruses." Analytical Chemistry 93, no. 2: 792-800.
Airborne pathogens causing infectious diseases are often highly transmittable between humans. Therefore, an airborne pathogen-monitoring system capable of on-site detection and identification would aid tremendously in preventing and controlling the early stages of pathogen spread. Here, we describe an integrated sampling/monitoring platform for on-site and real-time detection of airborne viruses. We used MS2 bacteriophage and avian influenza virus (AIV) H1N1 to evaluate bioaerosol sampling and detection performance of the platform. Our results show that, within 20 min, aerosolized viruses can be detected using the signal of near-infrared (NIR)-to-NIR nanoprobes. The pretreatment of the sampling pad improved the transfer efficiency of MS2 viruses to the detection zone, compared to an untreated pad. Our platform could detect concentrations as low as 104.294 50% egg infectious dose (EID50)/m3 AIVs collected from a cloacal swab sample (104.838 EID50/mL). These results indicate that our sampling/monitoring platform could be applied for the early detection of biological hazards in various fields.
InAe Lee; Youngung Seok; Huijin Jung; Byungjin Yang; Jiho Lee; Jaeyoung Kim; Heesoo Pyo; Chang-Seon Song; Won Choi; Min-Gon Kim; Joonseok Lee. Integrated Bioaerosol Sampling/Monitoring Platform: Field-Deployable and Rapid Detection of Airborne Viruses. ACS Sensors 2020, 5, 3915 -3922.
AMA StyleInAe Lee, Youngung Seok, Huijin Jung, Byungjin Yang, Jiho Lee, Jaeyoung Kim, Heesoo Pyo, Chang-Seon Song, Won Choi, Min-Gon Kim, Joonseok Lee. Integrated Bioaerosol Sampling/Monitoring Platform: Field-Deployable and Rapid Detection of Airborne Viruses. ACS Sensors. 2020; 5 (12):3915-3922.
Chicago/Turabian StyleInAe Lee; Youngung Seok; Huijin Jung; Byungjin Yang; Jiho Lee; Jaeyoung Kim; Heesoo Pyo; Chang-Seon Song; Won Choi; Min-Gon Kim; Joonseok Lee. 2020. "Integrated Bioaerosol Sampling/Monitoring Platform: Field-Deployable and Rapid Detection of Airborne Viruses." ACS Sensors 5, no. 12: 3915-3922.
The lateral flow immunosensor (LFI) is a widely used diagnostic tool for biomarker detection; however, its sensitivity is often insufficient for analyzing targets at low concentrations. Here, an electrochemiluminescent LFI (ECL-LFI) is developed for highly sensitive detection of troponin I (TnI) using Ru(bpy)32+ -loaded mesoporous silica nanoparticles (RMSNs). A large amount of Ru(bpy)32+ is successfully loaded into the mesoporous silica nanoparticles with excellent loading capacity and shows strong ECL signals in reaction to tripropylamine. Antibody-immobilized RMSNs are applied to detect TnI by fluorescence and ECL analysis after a sandwich immunoassay on the ECL-LFI strip. The ECL-LFI enables the highly sensitive detection of TnI-spiked human serum within 20 min at femtomolar levels (≈0.81 pg mL-1 ) and with a wide dynamic range (0.001-100 ng mL-1 ), significantly outperforming conventional fluorescence detection (>3 orders of magnitude). Furthermore, TnI concentrations in 35 clinical serum samples across a low range (0.01-48.31 ng mL-1 ) are successfully quantified with an excellent linear correlation (R2 = 0.9915) using a clinical immunoassay analyzer. These results demonstrate the efficacy of this system as a high-performance sensing strategy capable of capitalizing on future point-of-care testing markets for biomolecule detection.
Donggu Hong; Eun‐Jung Jo; Kihyeun Kim; Mun‐Beom Song; Min‐Gon Kim. Ru(bpy) 3 2+ ‐Loaded Mesoporous Silica Nanoparticles as Electrochemiluminescent Probes of a Lateral Flow Immunosensor for Highly Sensitive and Quantitative Detection of Troponin I. Small 2020, 16, e2004535 .
AMA StyleDonggu Hong, Eun‐Jung Jo, Kihyeun Kim, Mun‐Beom Song, Min‐Gon Kim. Ru(bpy) 3 2+ ‐Loaded Mesoporous Silica Nanoparticles as Electrochemiluminescent Probes of a Lateral Flow Immunosensor for Highly Sensitive and Quantitative Detection of Troponin I. Small. 2020; 16 (44):e2004535.
Chicago/Turabian StyleDonggu Hong; Eun‐Jung Jo; Kihyeun Kim; Mun‐Beom Song; Min‐Gon Kim. 2020. "Ru(bpy) 3 2+ ‐Loaded Mesoporous Silica Nanoparticles as Electrochemiluminescent Probes of a Lateral Flow Immunosensor for Highly Sensitive and Quantitative Detection of Troponin I." Small 16, no. 44: e2004535.
Diabetes mellitus is one of the most common chronic diseases worldwide. Generally, the levels of fasting or postprandial blood glucose and other biomarkers, such as glycated albumin, glycated hemoglobin, and 1,5-anhydroglucitol, are used to diagnose or monitor diabetes progression. In the present study, we developed a sensor to simultaneously detect the glucose levels and glycation ratios of human serum albumin using a lateral flow assay. Based on the specific enzymatic reactions and immunoassays, a spiked glucose solution, total human serum albumin, and glycated albumin were measured simultaneously. To test the performance of the developed sensor, clinical serum samples from healthy subjects and patients with diabetes were analyzed. The glucose level and glycation ratios of the clinical samples were determined with reasonable correlation. The R-squared values of glucose level and glycation ratio measurements were 0.932 and 0.930, respectively. The average detection recoveries of the sensor were 85.80% for glucose and 98.32% for the glycation ratio. The glucose level and glycation ratio in our results were crosschecked with reference diagnostic values of diabetes. Based on the outcomes of the present study, we propose that this novel platform can be utilized for the simultaneous detection of glucose and glycation ratios to diagnose and monitor diabetes mellitus.
Hangil Ki; Hyungjun Jang; Jusung Oh; Gyeo-Re Han; Hoyeon Lee; Sanghyo Kim; Min-Gon Kim. Simultaneous Detection of Serum Glucose and Glycated Albumin on a Paper-Based Sensor for Acute Hyperglycemia and Diabetes Mellitus. Analytical Chemistry 2020, 92, 1 .
AMA StyleHangil Ki, Hyungjun Jang, Jusung Oh, Gyeo-Re Han, Hoyeon Lee, Sanghyo Kim, Min-Gon Kim. Simultaneous Detection of Serum Glucose and Glycated Albumin on a Paper-Based Sensor for Acute Hyperglycemia and Diabetes Mellitus. Analytical Chemistry. 2020; 92 (17):1.
Chicago/Turabian StyleHangil Ki; Hyungjun Jang; Jusung Oh; Gyeo-Re Han; Hoyeon Lee; Sanghyo Kim; Min-Gon Kim. 2020. "Simultaneous Detection of Serum Glucose and Glycated Albumin on a Paper-Based Sensor for Acute Hyperglycemia and Diabetes Mellitus." Analytical Chemistry 92, no. 17: 1.
This paper-based sensor quantified glucose and 1,5-anhydroglucitol using enzyme-based colorimetric and elimination reaction in human urine.
Hyungjun Jang; Jusung Oh; Hangil Ki; Min-Gon Kim. Paper-based 1,5-anhydroglucitol quantification using enzyme-based glucose elimination. The Analyst 2020, 145, 5740 -5743.
AMA StyleHyungjun Jang, Jusung Oh, Hangil Ki, Min-Gon Kim. Paper-based 1,5-anhydroglucitol quantification using enzyme-based glucose elimination. The Analyst. 2020; 145 (17):5740-5743.
Chicago/Turabian StyleHyungjun Jang; Jusung Oh; Hangil Ki; Min-Gon Kim. 2020. "Paper-based 1,5-anhydroglucitol quantification using enzyme-based glucose elimination." The Analyst 145, no. 17: 5740-5743.
As a global shift continues to occur in high burden diseases toward developing countries, the importance of medical diagnostics based on point-of-care testing (POCT) is rapidly increasing. However, most diagnostic tests that meet clinical standards rely on high-end analyzers in central hospitals. Here, we report the development of a simple, low-cost, mass-producible, highly sensitive/quantitative, automated, and robust paper/soluble polymer hybrid-based lateral flow biosensing platform, paired with a smartphone-based reader, for high-performance POCT. The testing architecture incorporates a polymeric barrier that programs/automates sequential reactions via a polymer dissolving mechanism. The smartphone-based reader with simple opto-mechanical parts offers a stable framework for accurate quantification. Analytical performance of this platform was evaluated by testing human cardiac troponin I (cTnI), a preferred biomarker for the diagnosis of myocardial infarction, in serum/plasma samples. Coupled with catalytic/colorimetric gold-ion amplification, this platform produced results within 20 min, with a detection limit of 0.92 pg mL-1 and a coefficient of variation < 10%, which is equivalent to the performance of a high-sensitivity standard analyzer, and operated within acceptable levels stipulated by clinical guidelines. Moreover, cTnI clinical sample tests indicate a high correlation (r = 0.981) with the contemporary analyzers, demonstrating the clinical utility of this platform in high-performance POCT.
Gyeo-Re Han; Hee Joon Koo; Hangil Ki; Min-Gon Kim. Paper/Soluble Polymer Hybrid-Based Lateral Flow Biosensing Platform for High-Performance Point-of-Care Testing. ACS Applied Materials & Interfaces 2020, 12, 34564 -34575.
AMA StyleGyeo-Re Han, Hee Joon Koo, Hangil Ki, Min-Gon Kim. Paper/Soluble Polymer Hybrid-Based Lateral Flow Biosensing Platform for High-Performance Point-of-Care Testing. ACS Applied Materials & Interfaces. 2020; 12 (31):34564-34575.
Chicago/Turabian StyleGyeo-Re Han; Hee Joon Koo; Hangil Ki; Min-Gon Kim. 2020. "Paper/Soluble Polymer Hybrid-Based Lateral Flow Biosensing Platform for High-Performance Point-of-Care Testing." ACS Applied Materials & Interfaces 12, no. 31: 34564-34575.
Several tropical fever viruses transmitted by mosquitoes including zika, dengue, and chikungunya, are becoming a serious problem in global public health. Simple diagnostic tools in early stages are strongly required to monitor and prevent these diseases. Paper diagnostic platforms can provide a solution for these needs, with integration of fluidic control techniques and isothermal amplification methods. Here, we demonstrate a Lab-on-paper for all-in-one molecular diagnostics of zika, dengue, and chikungunya virus from human serum. The entire process of nucleic acid testing that involves sampling, extraction, amplification, and detection is simply operated on a single paper chip. Based on the engineered structure of paper materials and dried chemicals on the all-in-one chip, serum samples containing the target virus RNA were simply added by automatic flow from distilled water injection. Target RNA molecules were concentrated on the binding pad with chitosan and then transported to reaction pads following a pH increase for specific reverse transcription loop-mediated isothermal amplification with fluorescence signal generation. Three targets, zika virus, dengue virus, and chikungunya virus, in human serum were simultaneously detected on the all-in-one paper chip within 60 min at 65 °C. The all-in-one paper chip can be used as a real-time quantitative assay for 5–5000 copies of zika virus RNA. This all-in-one device was successfully used with 5 clinical specimens of zika and dengue virus from real patients. We believe that the proposed all-in-one paper chip can provide a portable, low-cost, user-friendly, sensitive, and specific NAT platform with great potential in point-of-care diagnostics.
Youngung Seok; Bhagwan S. Batule; Min-Gon Kim. Lab-on-paper for all-in-one molecular diagnostics (LAMDA) of zika, dengue, and chikungunya virus from human serum. Biosensors and Bioelectronics 2020, 165, 112400 .
AMA StyleYoungung Seok, Bhagwan S. Batule, Min-Gon Kim. Lab-on-paper for all-in-one molecular diagnostics (LAMDA) of zika, dengue, and chikungunya virus from human serum. Biosensors and Bioelectronics. 2020; 165 ():112400.
Chicago/Turabian StyleYoungung Seok; Bhagwan S. Batule; Min-Gon Kim. 2020. "Lab-on-paper for all-in-one molecular diagnostics (LAMDA) of zika, dengue, and chikungunya virus from human serum." Biosensors and Bioelectronics 165, no. : 112400.
A simple, universal, and sensitive colorimetric biosensor for detecting of various biomarkers was devised using a target-specific DNA aptamer, as the recognition element, and engineered with streptavidin-fusion replication protein A 70 kDa (RPA70A) linked to biotin-horseradish peroxidase, as the colorimetric element. To improve sensitivity and stability compared to other colorimetric sensing platforms, we developed a novel detection strategy by integrating a newly selected heterogeneous sandwich DNA aptamer and protein engineering in this study. The proposed method is based on a change in color from colorless to blue due to the interaction of the aptamer with RPA70A in the presence of the target; this color change could be observed by the naked eye or measured with a UV–vis spectrometer. We confirmed its high sensitivity and specificity for two model targets using their aptamers under optimal experimental conditions. In addition, the feasibility of the assay was investigated in clinical samples containing NPs of influenza A or B virus. These results suggest that our detection system developed herein can be universally applied to the diagnosis of various diseases owing to its stability, sensitivity, and specificity.
Juyoung Kang; Gyuho Yeom; Hyungjun Jang; Chin- Ju Park; Min- Gon Kim. Highly sensitive and universal detection strategy based on a colorimetric assay using target-specific heterogeneous sandwich DNA aptamer. Analytica Chimica Acta 2020, 1123, 73 -80.
AMA StyleJuyoung Kang, Gyuho Yeom, Hyungjun Jang, Chin- Ju Park, Min- Gon Kim. Highly sensitive and universal detection strategy based on a colorimetric assay using target-specific heterogeneous sandwich DNA aptamer. Analytica Chimica Acta. 2020; 1123 ():73-80.
Chicago/Turabian StyleJuyoung Kang; Gyuho Yeom; Hyungjun Jang; Chin- Ju Park; Min- Gon Kim. 2020. "Highly sensitive and universal detection strategy based on a colorimetric assay using target-specific heterogeneous sandwich DNA aptamer." Analytica Chimica Acta 1123, no. : 73-80.
Lateral flow assays (LFAs) have become the most common biosensing platforms for point-of-care testing due to their compliance with the ASSURED (affordable, sensitive, specific, user-friendly, rapid/robust, equipment-free, and deliverable to end-users) guidelines stipulated by the World Health Organization. However, the limited analytical sensitivity and low quantitative capability of conventional LFAs, which use gold nanoparticles (AuNPs) for colorimetric labeling, have prevented high-performance testing. Here, we report the development of a highly sensitive chemiluminescence (CL)-based LFA involving AuNPs conjugated with aldehyde-activated peroxidase and antibody molecules—i.e., AuNP-(ald)HRP-Ab—as a new conjugation scheme for high-performance testing in LFAs. When paired with the CL-based signal readout modality, the AuNP-(ald)HRP-Ab conjugate resulted in 110-fold enhanced sensitivity over the colorimetric response of a typical AuNP-Ab conjugate. To evaluate the performance of the CL-based LFA, we tested it with human cardiac troponin I (cTnI; a standard cardiac biomarker used to diagnose myocardial infarction) in standard and clinical serum samples. Testing the standard samples revealed a detection limit of 5.6 pg·mL−1 and acceptably reliable precision (with a coefficient of variation of 2.3%–8.4%), according to clinical guidelines. Moreover, testing the clinical samples revealed a high correlation (r = 0.97) with standard biochemical analyzers, demonstrating the potential clinical utility of the CL-based LFA for high-performance cTnI testing.
Gyeo-Re Han; Min-Gon Kim. Highly Sensitive Chemiluminescence-Based Lateral Flow Immunoassay for Cardiac Troponin I Detection in Human Serum. Sensors 2020, 20, 2593 .
AMA StyleGyeo-Re Han, Min-Gon Kim. Highly Sensitive Chemiluminescence-Based Lateral Flow Immunoassay for Cardiac Troponin I Detection in Human Serum. Sensors. 2020; 20 (9):2593.
Chicago/Turabian StyleGyeo-Re Han; Min-Gon Kim. 2020. "Highly Sensitive Chemiluminescence-Based Lateral Flow Immunoassay for Cardiac Troponin I Detection in Human Serum." Sensors 20, no. 9: 2593.
Detection of food adulteration is a challenge. However, the identification of adulterated meat in processed products is important for health and personal preference. Mitochondrial genomic DNA (mtDNA) is a good candidate for reliable identification of meat ingredients; however, the extraction of mtDNA from processed products is a bottleneck for development of detection strategies. Therefore, we constructed a rapid (~5 min) mtDNA extraction device. mtDNAs from different meat samples, such as pork (Sus scrofa), chicken (Gallus gallus), and beef (Bos taurus), were successfully detected in up to 0.1% adulterated animal species. We believe that the proposed strategy could be applied to detect animal species from processed meat products to reduce fraudulent practices.
Bhagwan S. Batule; Youngung Seok; Min-Gon Kim. An innovative paper-based device for DNA extraction from processed meat products. Food Chemistry 2020, 321, 126708 .
AMA StyleBhagwan S. Batule, Youngung Seok, Min-Gon Kim. An innovative paper-based device for DNA extraction from processed meat products. Food Chemistry. 2020; 321 ():126708.
Chicago/Turabian StyleBhagwan S. Batule; Youngung Seok; Min-Gon Kim. 2020. "An innovative paper-based device for DNA extraction from processed meat products." Food Chemistry 321, no. : 126708.
Several types of biosensors have been developed to detect a wide variety of human diseases. Immunosensors are classified as the most representative of all biosensors. They are based on antibodies that selectively recognize specific analytes and have high specificity and sensitivity. However, there are limitations to the types of substances that can be detected, and it is sometimes difficult to achieve sufficient sensitivity without additional amplification steps. To overcome these problems, novel immunosensors are being developed that combine DNA-based high signal amplification systems. These technologies ameliorate the low sensitivity of existing immunosensors by using DNA probes that can bind directly to targets as bioreceptors or act as signal amplifiers. In this review, we will discuss immunodetection methods that use DNA-based technologies on laboratory-scale and advanced point-of-care testing (POCT) that employ these technologies for high performance analyses.
Juyoung Kang; Min-Gon Kim. Advancements in DNA-assisted Immunosensors. BioChip Journal 2020, 14, 18 -31.
AMA StyleJuyoung Kang, Min-Gon Kim. Advancements in DNA-assisted Immunosensors. BioChip Journal. 2020; 14 (1):18-31.
Chicago/Turabian StyleJuyoung Kang; Min-Gon Kim. 2020. "Advancements in DNA-assisted Immunosensors." BioChip Journal 14, no. 1: 18-31.
We developed two advanced lateral flow immunoassays (LFIAs) for the simultaneous determination of total human serum albumin and glycated albumin concentrations with wide detection ranges and improved glycated albumin selectivity.
Hangil Ki; Jusung Oh; Gyeo-Re Han; Min-Gon Kim. Glycation ratio determination through simultaneous detection of human serum albumin and glycated albumin on an advanced lateral flow immunoassay sensor. Lab on a Chip 2020, 20, 844 -851.
AMA StyleHangil Ki, Jusung Oh, Gyeo-Re Han, Min-Gon Kim. Glycation ratio determination through simultaneous detection of human serum albumin and glycated albumin on an advanced lateral flow immunoassay sensor. Lab on a Chip. 2020; 20 (4):844-851.
Chicago/Turabian StyleHangil Ki; Jusung Oh; Gyeo-Re Han; Min-Gon Kim. 2020. "Glycation ratio determination through simultaneous detection of human serum albumin and glycated albumin on an advanced lateral flow immunoassay sensor." Lab on a Chip 20, no. 4: 844-851.
The recent outbreaks of mosquito-borne diseases (e.g., zika, dengue, and chikungunya) increased public health burden in developing countries. To control the spread of these infectious diseases, a simple, economic, reliable, sensitive, and selective diagnostic platform is required. Considering demand for affordable and accessible methods, we have demonstrated a two-step strategy for extraction and detection of viral RNAs of infectious diseases within 1 h. Ready-to-use devices for viral RNA extraction and detection were successfully fabricated using paper as a substrate. Viral RNA (e.g., zika, dengue, and chikungunya) was captured and eluted using a handheld RNA extraction paper-strip device, and another paper-chip device was used for reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay with a detection limit of a single copy and 10 copies of viral RNA in phosphate buffer solution (PBS) and serum, respectively. With these proposed devices, we have detected viral RNAs of zika and dengue in clinical human serum samples. The proposed paper-based extraction and detection platforms could be employed for detection of infectious viral diseases from complex clinical samples in resource-limited settings.
Bhagwan S. Batule; Youngung Seok; Min-Gon Kim. Paper-based nucleic acid testing system for simple and early diagnosis of mosquito-borne RNA viruses from human serum. Biosensors and Bioelectronics 2019, 151, 111998 .
AMA StyleBhagwan S. Batule, Youngung Seok, Min-Gon Kim. Paper-based nucleic acid testing system for simple and early diagnosis of mosquito-borne RNA viruses from human serum. Biosensors and Bioelectronics. 2019; 151 ():111998.
Chicago/Turabian StyleBhagwan S. Batule; Youngung Seok; Min-Gon Kim. 2019. "Paper-based nucleic acid testing system for simple and early diagnosis of mosquito-borne RNA viruses from human serum." Biosensors and Bioelectronics 151, no. : 111998.