Nuclear genes of euglenids and marine diplonemids harbor atypical, nonconventional introns which are not observed in the genomes of other eukaryotes. Nonconventional introns do not have the conserved borders characteristic for spliceosomal introns or the sequence complementary to U1 snRNA at the 5' end. They form a stable secondary structure bringing together both exon/intron junctions, nevertheless, this conformation does not resemble the form of self-splicing or tRNA introns. In the genes studied so far, frequent nonconventional introns insertions at new positions have been observed, whereas conventional introns have been either found at the conserved positions, or simply lost. In this work, we examined the order of intron removal from Euglena gracilis transcripts of the tubA and gapC genes, which contain two types of introns: nonconventional and spliceosomal. The relative order of intron excision was compared for pairs of introns belonging to different types. Furthermore, intermediate products of splicing were analyzed using the PacBio Next Generation Sequencing system. The analysis led to the main conclusion that nonconventional introns are removed in a rapid way but later than spliceosomal introns. Moreover, the observed accumulation of transcripts with conventional introns removed and nonconventional present may suggest the existence of a time gap between the two types of splicing. The existence of conventional spliceosomal introns in genes of eukaryotic organisms is a well-known theorem. However, genes of the unicellular algae group, euglenids, contain also another type of introns, so-called nonconventional ones. They lack canonical borders, a feature most characteristic for conventional introns and form a stable secondary structure bringing together their ends. Along with the increasing popularity of whole genome studies, nonconventional introns were also disclosed in the genes of other protists, diplonemids. In this study we were particularly interested which introns–conventional or nonconventional–are removed earlier from euglenids’ pre-mRNA. To track this process we analyzed transcripts of the two Euglena gracilis genes. The relative order of intron excision was compared for pairs of introns belonging to different types. We also surveyed thousands of intermediate products of splicing using the Next-Generation Sequencing system. Summarizing the results of both experiments, we proved that spliceosomal introns are removed at an earlier stage of pre-mRNA maturation than nonconventional ones.

Background/Objectives: With increasing knowledge of the role of the microbiota in health and disease, the need for the reliable simulation of its behavior in response to various factors, such as diet and probiotic administration in in vitro conditions, has emerged. Although many studies utilize developed systems, data on how accurately these systems represent individual microbiota responses are scarce. Methods: In the present study, the Simulator of Human Intestinal Microbial Ecosystem (SHIME^®) was exposed to experimental conditions mimicking the application of probiotics and dietary changes in the study participant. Next-generation 16S rRNA sequencing was used to reveal the structure of the microbial communities in the analyzed samples. Results: Analysis of 17 samples revealed that predominantly diet and, to a lesser extent, probiotics had a divergent effect on the microbiota’s fluctuations dependent on the culture environment. Despite this, results from both in vitro and in vivo conditions aligned well with previously published data on the expected impact of dietary changes on the intestinal microbial community. Conclusions: The anecdotal evidence presented in this study suggested that current in vitro technology enables the reproduction of some of the microbiota responses that are well known from in vivo research. However, further work is required to enable simulations of an individual microbiota.

In this study, we decided to test the hypothesis that the fungal biostarter M. flavus used during a 21-day beef dry-aging process significantly impacts the composition of other microorganisms, the profile of volatile compounds, meat hardness characteristics, and, consequently, the sensory quality. The experiments were performed on samples derived from animals crossbred between Holstein–Fresian cows and meat breed bulls. Two groups of samples were studied, including the control group, without biostarter, and a group inoculated with the M. flavus biostarter. Both sample groups were seasoned for 21 days in the dry-aging fridge. The physicochemical parameters (pH, color parameters), the chemical composition of muscle, the determination of the shear force, the profile of volatile compounds (VOCs), and the sensory quality were evaluated after aging. During this study, classical microbiological methods were used to investigate the influence of fungal biostarters on the growth and survival of bacteria and other fungi (e.g., yeasts) during the dry-aging process of beef (DAB). The M. flavus biostarter improved the sensory quality of DAB, allowing high sensory quality to be achieved after just 21 days. This is likely due to the diverse VOCs produced by the fungus, including 1-tetradecanol, 2-nonenal, trans-2-undecenoic acid, and the following esters: formic acid hexyl ester, 10-undecenoic acid methyl ester, and 4-methylpentanoic acid methyl ester. The presence of the biostarter had no significant effect on the number of the bacteria or the survivability of the L. monocytogenes on the meat’s surface in laboratory conditions.

The dry ageing is a historically relevant method of meat preservation, now used as a way to produce the dry-aged beef (DAB) known for its pronounced flavour. Partially responsible for the taste of the DAB may be various microorganisms that grow on the surface of the meat. Historically, the fungal species colonising the DAB were described as members of the genera Thamnidium and Mucor. In this study we used both culture based approach as well as ITS2 rDNA metabarcoding analysis to investigate the fungal community of the DAB, with special emphasis on the mucoralean taxa. Isolated fungi were members of 6 different species from the family Mucoraceae, belonging to the genera Mucor and Helicostylum. Metabarcoding data provided supplementary information regarding the presence of other fungi including those from the Thamnidium genus. In both approaches used in this study isolates closely related to the Mucor flavus strain CBS 992.68 dominated.

Powstanie mitochondriów było jednym z najważniejszych wydarzeń w historii życia na Ziemi. Wchłonięta komórka bakteryjna, przekształcona następnie w mitochondrium zachowała swój genom, który uległ następnie licznym modyfikacjom. Na skutek masowej utraty i licznych transferów genów do genomu jądrowego autonomiczna bakteria uległa ostatecznie przekształceniu w organellum, które znamy dzisiaj. W efekcie zmian zachodzących niezależnie w różnych liniach ewolucyjnych obserwujemy bardzo dużą różnorodność genomów mitochondrialnych na poziomie struktury jak i pod względem zawartości genów. W większości przypadków mitochondrialny DNA zachował kolisty kształt, nie jest to jednak jedyna jego obserwowana forma – w mitochondriach niektórych eukariontów obserwujemy cząsteczki liniowe, a w skrajnych przypadkach, w zredukowanych organellach pochodzenia mitochondrialnego, genom został całkowicie utracony. W niniejszym artykule omawiamy różnorodność struktur genomów mitochondrialnych w obrębie największych grup Eukarya.