The growing demand for high-value animal protein must be met using sustainable means that optimize the utilization of nutrients, especially nitrogen (N) so that excreta do not over-fertilize fields and end up causing soil acidification, waterway eutrophication and greenhouse gas emissions. Malodorous N compounds can cause respiratory diseases and poor growth in livestock. The increasing availability of feed-grade amino acids makes it possible to formulate low-protein diets for broilers and thereby reduce N excretion. However, published studies of the effects of such diets on broiler growth performance have been based on reducing CP contents gradually in a variety of ways that have given inconsistent results. Since the amount of published data is now large, a meta-analysis was performed in order to categorize diet formulation strategies and quantify their impact on N balance, water consumption, litter moisture, plasma uric acid. This showed that lowering the CP content of broiler diets generally means replacing some soybean meal with corn and hence increasing the starch content. However, since soybean meal is also a source of potassium, this reduces electrolyte balance. Lowering the CP content from 19% to 17% is associated with a 29% reduction of N excretion in broilers aged 0-21 d, and a 7% increase in N efficiency (N retention/N intake). Reducing the CP content from 19% to 17% decreases daily water consumption by 20.6 mL/bird, litter moisture by 2.2% and plasma uric acid by 0.56 mg/dL. This meta-analysis improves our understanding of the low-protein strategy and allows us to quantify its impact on N balance, litter quality and uric acid. It shows that managing N excretion is wholly beneficial and reduces litter wetness.

There is large variation in amino acids requirements among pigs, hence feeding pigs individually with daily tailored diets or in groups with a single feed may require different levels of nutrients. Thus, the response to different threonine levels (70%, 85%, 100%, 115%, and 130% of the ideal threonine:lysine protein ratio of 0.65) was studied in growing pigs raised in a conventional group phase-feeding (GPF) system or fed individually using individual precision-feeding (IPF) techniques. In a 21-day trial, 110 barrows (25 ± 0.80 kg body weight) were housed in the same room and fed using electronic feeders. Five pigs per treatment were slaughtered at the end of the trial. Threonine intake increased linearly for the IPF and GPF pigs (P < 0.05). Lysine intake was similar across the treatments. Average daily gain, gain:feed ratio, and protein deposition were affected linearly by threonine level (P < 0.05) in both feeding systems. Protein deposition in the GPF pigs was maximized at 150 g/d and a 0.65 threonine:lysine ratio, whereas protein deposition increased linearly in the IPF pigs. Plasma Met and serine levels were 11 and 7% higher, respectively, in the IPF pigs than in the GPF pigs (P < 0.05). Dietary threonine increased (P < 0.05) threonine concentration in the longissimus dorsi in a quadratic manner in the IPF pigs, whereas there was no effect in the GPF pigs. Longissimus dorsi collagen decreased as dietary threonine increased in the IPF and GPF pigs (P < 0.10). Carcass muscle crude protein was 2% higher in the GPF pigs than in the IPF pigs (P < 0.05). Individual pigs are able to modulate growth and the composition of growth according to threonine intake. The average amino acid ratio value that is currently used for GPF cannot be used for IPF.

This study investigated the effect of reducing dietary crude protein (CP) content in the grower and finisher diets of broiler chickens on breast meat quality, muscle protein functionality, growth, carcass yield, and meat yield. To achieve this, a total of 1,269 one-day-old male Ross 308 chicks were fed 1 of 3 diets replicated 9 times each in a randomized complete block design with 9 blocks. The diets included a control (20.4% and 19.5% CP in the grower and finisher phase, respectively), a diet with a 1.5% reduction (CP-1.5%) and a diet with a 3.0% reduction (CP-3.0%) in CP content in both the grower and finisher phases. At the end of the experiment, the reduced-CP diets had no impact on body weight, feed intake, or feed conversion ratio. However, reduced-CP diet resulted in reduced (P < 0.001) total nitrogen intake (-7.46 and -11.94% in CP-1.5% and CP-3.0%, respectively). Breast meat quality was assessed (n = 36 birds/group), and the experimental diets were associated with a slightly increased (P = 0.07) ultimate pH (5.75, 5.79, and 5.81 for the control, CP-1.5%, and CP-3.0%, respectively). Breast fillets from the CP-1.5% and CP-3.0% groups had lower yellowness (b*, P < 0.001) and lower cooking loss (CL, P < 0.001) values than the control. Moreover, the solubility, emulsion activity, and stability indices of the sarcoplasmic and myofibrillar fractions of muscle proteins were not influenced by the diets. CP-1.5% and CP-3.0% diets were associated with an increased (P < 0.001) breast yield (18.39, 19.21, and 19.61% for the control, CP-1.5%, and CP-3.0%, respectively) while leg yield remained unchanged. Additionally, breast meat nutritional properties including protein and lipid contents were not impacted by the experimental diets. In conclusion, the CP content in the grower and finisher diets of broiler chickens can be reduced by as much as 3.0% without detrimental effects on performance or on meat quality as long as birds' amino acid requirements are adequately met.

Nutritional values of ingredients have been and still are the subject of many studies to reduce security margins of nutrients when formulating diets to reduce feed cost. In most studies, pigs are fed a limited amount of feed in a limited number of meals that do not represent how pigs are fed in commercial farm conditions. With free access to feed, pigs follow their intrinsic feeding behaviour. Feed intake is regulated by satiety and satiation signals. Reducing the feed intake level or feeding frequency can affect digestibility and transit time and induce metabolic changes. To reduce feed costs, alternative ingredients that are frequently rich in dietary fibre are added to diets. Fibre acts on the digestion process and transit time by decreasing energy density and causing viscosity. Various analyses of fibre can be realised, and the measured fibre fraction can vary. Exogenous enzymes can be added to counteract the effect of fibre, but digestive tract conditions, influenced by meal size and frequency, can affect the efficiency of supplemented enzymes. In conclusion, the frequency and size of the meals can affect the digestibility of nutrients by modulating gastrointestinal tract conditions (pH and transit time), metabolites (glucose and short-chain fatty acids) and hormones (glucagon-like peptide 1 and peptide tyrosine tyrosine).

An infection by protozoa Eimeria spp. can cause coccidiosis, which negatively affects broiler chicken performance and causes economic and production losses. To understand the effect of coccidiosis on broilers' performance, we evaluated the independent variables and their interactions on the severity of coccidiosis in broilers that cause variation (Δ) of average daily feed intake (ADFI), average daily gain (ADG), and gain per feed (G:F) of broiler chicks using a meta-analysis approach. A database of 55 papers describing 63 experiments was gathered; broilers were challenged by Eimeria species (E. acervulina, E. maxima, E. tenella, and mixed) and at least 2 variables among ADFI, ADG, and feed conversion ratio (FCR) were studied. The variation induced by the challenge was calculated relative to the control group of each experiment. The indirect factors evaluated were days postinfection (DPI), Eimeria type and dose, infection age (IA), bird's mean age in the analyzed period, genetic line, sex, and whether they were raised in a cage or a pen. Graphical, correlation, and variance analyses were performed to evaluate the form of the responses. Then, a linear plateau model was adjusted for each response variable as a function of DPI to determine the consequences of the disease on the variation of performance over time after infection. The impact of the infection challenge on the variation of performance vs. nonchallenge broilers was only impacted by DPI (P < 0.05). The adjustment of the data with the linear plateau model allows us to determine the host response to the coccidiosis disease at different stages. At 5 DPI (acute phase), ΔADFI, ΔADG, ΔG:F were of -19.0; -39.8, and -25.5, respectively. After almost 13 DPI birds achieved the recovery phase for all variables with Δ varying from -19 to -3.75% for ADFI, from -39.8 to -10.5% for ADG, and from -25.5 to -7.24% for G:F. The Eimeria impact was higher in ADG than ADFI in all periods due to Eimeria aggressive action form causing lesions in gut epithelial reducing the use of nutrients and energy. The results can be used as a quantitative approach to determine the consequences of Eimeria spp. on broiler performance.

Eimeria infections impair digestive tract capacity and barrier function leading to poor growth and feed efficiency. A meta-analysis approach was used to evaluate and quantify impact of Eimeria infection on the apparent ileal digestibility (AID) of amino acids (AA) in broiler chickens. A database composed of 6 articles with a total of 21 experiments was built for the effect of challenge type (a mix of Eimeria spp. vs. E. acervulina) and subdatabase of 3 articles with a total of 15 experiments for the effect of E. acervulina dose response. Regression models were fitted with the mixed model procedure in Minitab 19 with fixed effects of challenge, species, and their interactions. For the sub database, the mixed model procedure was used to fit regression models and identify a linear or quadratic response to dose. Challenge decreased AID (P < 0.05) of both dispensable and indispensable AA except for Trp. Specifically, the largest depression was observed for Cys, Thr, Tyr, Ala, and Val with the magnitude of difference of 8.7, 5.4, 5.2, 5.1, and 4.9%, respectively for challenged vs. unchallenged birds. The type of challenge affected (P < 0.05) AID of AA with exception of Cys, Tyr, Ala, Ser, Leu, Asp, Gly, and Pro. E. acervulina challenge had larger negative effects on AID of Ile, Leu, and Val. Moreover, E. acervulina linearly decreased (P < 0.05) AID of all indispensable and dispensable AA except for Trp and quadratically (P < 0.05) decreased AID of all AA except Cys, Met, Arg, and Trp. The largest linear decrease due to E. acervulina dose was seen for AID of Cys, followed by Ala, Val, Thr, and Ile. Although, AID of Trp was not affected by E. acervulina challenge, mixed Eimeria species challenge decreased (P < 0.05) AID of Trp. Overall, the results confirmed that an Eimeria infection negatively impacted AA digestibility/utilization. The ranking of the most affected AA suggested ground for nutritional intervention during subclinical field Eimeria infections or vaccination programs.