The genus Pityocera Giglio-Tos is revised based on examination of external morphology and genitalia of type material and specimens from Brazilian and foreign institutions. Five currently valid species in three subgenera are recognized: P. (Elaphella) cervus (Wiedemann, 1828); P. (Pityocera) festai Giglio-Tos, 1896; P. (Pseudelaphella) nana (Walker, 1850); P. (Pseudelaphella) nigribasis Fairchild, 1964; P. (Pseudelaphella) patellicornis (Kröber, 1930). Five new species are described: P. (Pseudelaphella) barrosi Gorayeb & Krolow sp. nov. (Brazil: Mato Grosso do Sul); P. (Pseudelaphella) gorayebi Limeira-de-Oliveira & Krolow sp. nov. (Brazil: Maranhão, Tocantins and Bahia); P. (Pseudelaphella) pernaquila Gorayeb & Krolow sp. nov. (Brazil: Pará and Rondônia); P. (Pseudelaphella) rhinolissa Krolow & Henriques sp. nov. (Brazil: Pará, Maranhão, Tocantins, Rondônia, Mato Grosso, Goiás and Mato Grosso do Sul; Bolivia: Santa Cruz); P. (Pseudelaphella) ecuadorensis Buestán & Krolow sp. nov. (Ecuador: Manabí, Guayas, Santa Elena and Loja). We provide diagnosis, descriptions, redescriptions, distribution records, illustrations and discussion for all species, as well as a key for identification of species.

Elephantotus, a new genus of Tabanidae from the Amazon coast, Brazil, is described based on a new species E. tracuateuensis. Five females were collected in Pará State, and a male in Maranhão State. Arguments are presented for separating the new genus from Dasybasis, as well as the possibility of its occurrence being related to the nesting sites of coastal birds. The new species is characterized by its large size (x = 2.15 cm, n = 5 females), glabrous eyes, reddish-brown tegument, light brown frontal callus not touching the edges of the eyes, extending up to the vertex that has traces of ocelli, basal plate of the antennal flagellum with obtuse angle, without a tooth or spine, wings hyaline, with brown basal cells, without appendix in the fork of vein R4 +5, and genital furca wide with extended flaps.

Tabanus pseudonebulosus, new species, is described based on 217 female specimens and 5 males collected in Venezuela (Guarico, Aguaio, Santa Elena, and Palmarito) and in Brazil (Parcí: Santana do Araguaia, Serra das Andorinhas in São Geraldo do Araguaia, Serra Norte Carajás and Gorotire; Amazonas: Canutama; Mato Grosso do Sul: Corumbá), mostly in open vegetation, scrub forest and in rocky environments. Observed intraspecific variation in the specimens is presented and related to collection localities, and morphological differences are diagnosed in relation to the similar species Tabanus nebulosus DeGeer 1776 and T. importunus Wiedemann 1828. Morphometrical data and indices for specimens in different localities are provided. Drawings and photo of the new species are presented.

Nymphs of three species of the genus Anacroneuria Klapálek, 1909, A. marlieri Froehlich, 2001; A. minuta Klapálek, 1922, and A. manauensis Ribeiro-Ferreira, 2001 are described. The associations of nymphs with adults were made by rearing. The known geographical distribution in Brazil of these three species was expanded.

Dichelacera (Dichelacera) gemmae sp.n. is described based on 28 females collected in open vegetation of 'cerrado', in Carolina municipality, South of Maranhão state, Brazil.

The last instar nymph of Anacroneuria singularis Righi-Cavallaro & Lecci is described and figured from reared specimens collected in Para State, Brazil, a new locality record for Brazil. The nymphs of this species differ from other known species by the uniformly dark brown anterior of the head and the ochraceous spots of the pronotum.