Mesenchymal stem cells have been widely used in the treatment of various chronic diseases. The objective of this survey was to evaluate the therapeutic and regenerative potential of stem cells from adipose tissue (ASCs) in the milk production recovery repair of tissue injury in mastitis goats treated with antimicrobial agents prior to cell therapy. After the diagnosis of mastitis and treatment with gentamicin, eight lactating goats were selected for cellular and subsequent therapy, physical-chemical analysis of milk, ultrasonographic and histopathological examinations. The ASCs were taken from the subcutaneous fat of a young goat cultivated in vitro, marked with Qdots-655 and injected in the left mammary gland, being the right mammary gland used as the control. After 30 days the ultrasonographic and histopathological analyzes were repeated and, in the first lactation period, the physical-chemical analysis of the milk was reapeated. Before the cellular therapy, the physical-chemical quality of the milk was compromised and the ultrasonographic and histopathological analysis revealed a chronic inflammatory process and fibrous tissue. The marking of the ASCs with Qdots enabled the tracking, by fluorescence microscopy (BX41-OLYMPUS), in the mammary tissue. In the ASCs therapy, cultures showed high cellularity and characteristics favorable to preclinical studies; with the therapy the physical-chemical parameters of the milk, fat, protein, temperature and pH showed significant differences among the groups; five animals treated with ASCs reconstituted the functionality of the gland and the connective tissue reduced in quantity and inflammatory infiltrate cells. ASCs have potential for the possible regeneration of fibrous mastitis lesions in the mammary gland, however, it would be necessary to increase injection time for the histopathological analysis, since the reconstitution of the glandular acini within the assessed period was not finalized. ASCs can be used to reestablish milk production in goat with chronic mastitis repair mammary lesions, with potential to be a promising clinical alternative for animal rehabilitation for productivity.

Studies on canine babesiosis in northeastern Brazil are scarce, although the weather conditions in this region are favorable for the development of the tick vector. This study determined the prevalence of Babesia vogeli in dogs sampled in Teresina, state of Piauí, northeast Brazil, using direct and indirect diagnostic methods and performed a phylogenetic analysis of 18S rRNA sequences. A total of 315 dogs were screened during routine care regardless of clinical suspicion. Blood was collected by jugular venipuncture to perform indirect immunofluorescence assay (IFA) and polymerase chain reaction (PCR) and for parasite screening in peripheral blood smears. Positivity was 2.2% (7/315) by microscopy, 4.8% (15/315) by PCR, and 48.6% (153/315) by IFA. PCR amplified a 602-bp fragment of the piroplasmid 18S rRNA gene, and sequence alignment and analysis revealed 99% homology with B. vogeli isolates from other regions of Brazil and other countries. In addition, there was high variability among sequences from other northeast states of Brazil. This study is the first to perform the molecular analysis of B. vogeli in Piauí. The results demonstrate that canine babesiosis is endemic in dogs sampled in Teresina and that PCR may be the method of choice to perform parasite screening in this region.

Antibiotics can contribute to bacterial resistance, posing a significant public health concern. Synbiotics represent an effective alternative to antibiotics, promoting the balance of intestinal microbiota and creating a conducive environment for beneficial bacteria growth. This study aimed to assess the utility of the synbiotic supplement (+Poultry) as a substitute for antibiotics in broiler diets from 1 to 42 days of age. We evaluated its impact on performance during two stages: 1 to 21 days and 1 to 42 days, digestibility coefficients, metabolizable energy, duodenum and jejunum morphometry, carcass yield, and hematological parameters. A total of 440 Ross 308 strain broiler chicks, both males and females, were accommodated in 2m² cages from day 1 to day 42. The birds were randomly assigned to four treatments, with each treatment having five replications consisting of 22 birds, thus forming 20 experimental plots. The treatment groups were as follows: Basal diet (BD) without the addition of antibiotics or synbiotics, BD with 150ppm zinc bacitracin (BCZ), BD with 0.5kg/t of synbiotic, and BD with 1kg/t of synbiotic. The synbiotic supplement (+Poultry) used in this experiment, provided by Nutri+, contained amino acids, minerals, probiotic culture, prebiotic additives, and flavoring additives. Throughout the experiment, all birds were subjected to a weekly sanitary challenge, which involved using reused litter and water contaminated with litter. Treatment effects were observed on feed conversion (P = 0.0100) of broilers aged 1 to 21 days, indicating improved feed conversion in broilers consuming BD + 1kg/t synbiotic compared to BD alone. Consequently, we recommend the incorporation of a synbiotic supplement at a rate of 1kg/t as a viable antibiotic replacement for chickens aged 1 to 21 days. The tested synbiotic supplement shows promise as an antibiotic alternative in broiler diets at all life stages, maintaining both performance and bird health.

In vitro senescence of multipotent cells has been commonly associated with DNA damage induced by oxidative stress. These changes may vary according to the sources of production and the studied lineages, which raises questions about the effect of growing time on genetic stability. This study is aimed at evaluating the evolution of genetic stability, viability, and oxidative stress of bone marrow mesenchymal stem cells (MSCBMsu) and renal progenitor cells of the renal cortex (RPCsu) of swine (Sus scrofa domesticus) in culture passages. P2, P5, and P9 were used for MSCBMsu and P1, P2, and P3 for RPCsu obtained by thawing. The experimental groups were submitted to MTT, apoptosis and necrosis assays, comet test, and reactive substance measurements of thiobarbituric acid (TBARS), nitrite, reduced glutathione (GSH), and catalase. The MTT test curve showed a mean viability of and , respectively, for MSCBMsu and RPCsu. The percentages of MSCBMsu and RPCsu were presented, respectively, for apoptosis, an irregular and descending behavior, and necrosis, ascending and irregular. The DNA damage index showed higher intensity among the MSCBMsu in the P5 and P9 passages ( ). In the TBARS evaluation, there was variation among the lines of RPCsu and MSCBMsu, presenting the last most significant variations ( ). In the nitrite values, we identified only among the lines, in the passages P1 and P2, with the highest averages displayed by the MSCBMsu lineage ( ). The measurement of antioxidant system activity showed high standards, identifying differences only for GSH values, in the RPCsu lineage, in P3 ( ). This study suggests that the maintenance of cell culture in the long term induces lower regulation of oxidative stress, and RPCsu presents higher genetic stability and lower oxidative stress than MSCBMsu during in vitro expansion.

This experiment evaluated the effects of including buriti oil (BO) in the diet of broilers on growth performance, nutrient digestibility, carcass yield, meat quality, blood parameters, intestinal morphometry, the proliferation of collagen fibers in the skin and collagen concentration in the breast muscle. A total of 180 one-day-old Ross male chicks were distributed in a completely randomized experimental design with three treatments (0%, 1% and 2% BO) and six replications of 10 birds each. Nutrient digestibility was evaluated from 8 to 21 days, while the performance, carcass yield, meat cut yields, abdominal fat, meat quality, blood parameters, intestinal morphometry and skin histology of the birds were evaluated after 21 days. The inclusion of BO significantly affected (p ≤ 0.05) performance, digestibility, meat color, lymphocyte count, duodenal morphometry and collagen concentration. In addition, it increased the metabolizable energy of the diet and reduced the birds’ performance, indicating that the nutraceutical effect of BO improved nutrient use. However, it caused an excess of energy that had to be metabolized by the birds, consequently affecting their performance. BO increased the pigmentation of the breast meat and reduced the lymphocyte count, probably due to its antimicrobial action, consequently decreasing the recruitment of defense cells. An increase in the height of the crypt of the duodenum was observed as the inclusion of BO increased, with the opposite being observed in the jejunum. The inclusion of BO increased the histological proliferation of collagen from minimal (0%) to medium (1%) and moderate (2%), as well as the collagen concentration (CC = 35.933 + 4.677BO; R² = 0.80). Thus, due to functional/nutraceutical effects, adding BO in the diet of broilers can be a promising alternative to antibiotic growth promoters to improve poultry production.

As neoplasias cutâneas em cães, como histiocitomas, são comuns e geralmente aparecem na derme e tecido subcutâneo, mas podem ocorrer em outras áreas. O prognóstico e o tratamento dependem do grau histológico do tumor, com a ressecção cirúrgica sendo a mais eficaz. Nesse contexto, este trabalho teve como objetivo relatar um caso de exérese de tumor sugestivo de histiocitoma na região do cotovelo de um cão, macho, da raça Fila Brasileiro, que pesava 45 kg. Dessa forma, foi realizada uma avaliação clínica e exame físico, no qual o paciente apresentava sensibilidade à palpação na região do tumor. Além disso, realizaram-se exames complementares, a exemplo de hemograma, eletrocardiograma, bioquímica sérica, citologia e histologia. De acordo com esses exames realizados, foi constatado que o paciente precisaria de intervenção cirúrgica para a exérese da neoplasia. A abordagem cirúrgica foi feita executando-se uma incisão de forma elíptica com pouca margem de segurança. Em seguida, realizou-se a divulsão do tecido subcutâneo, que aderia a neoplasia à musculatura, e a remoção do tumor. A abolição de espaço morto foi realizada com fio de sutura Catgut de espessura zero e padrão de sutura simples contínuo. Após isso, fez-se a dermorrafia com fio de sutura Polipropileno de espessura zero e padrão de sutura Wolff com capton de equipo de soro fisiológico. Conclui-se que o tratamento cirúrgico foi eficaz, visto que o animal não precisou realizar o procedimento novamente.