Salinity has caused significant negative effects on agricultural production. This research is focused on the vulnerabilities of soil and water salinities on crop, fish, and livestock production across the Kalapara coastal belt of Bangladesh. Several parameters were measured as indicators of salinity. The electrical conductivity of water was found to be significant with TDS, F⁻, Cl⁻,SO42-, Na⁺, K⁺, Ca²⁺, Mg²⁺,NO2-, andPO43-. Chloride was found to be identical withSO42-, Na⁺, K⁺, Ca²⁺, Mg²⁺, Br⁻, andPO43-. Electrical conductivity, F⁻, Cl⁻,SO42-, Na⁺, K⁺, and Mg²⁺were all found to be higher than the recommended values. Similarly, soil conductivity was found significant with TDS, Cl⁻,SO42-, Na⁺, F⁻,NO2-,NO3-, andPO43-. Chloride in soil samples was found statistically identical withSO42-, Na⁺,NO3-, andPO43-. About 200 ha fodder crops areas are affected each year due to salinity. Ninety-two percent of the areas were found to be salinity affected in the 36 current cropping patterns. Twelve percent of marine fish and 25 percent of shrimp species have disappeared as a result of salinity. The negative impact of soil and water salinity on crops, fish, and livestock has been increasing in this coastal belt.

Bacterial biofilm plays a pivotal role in bioremediation of heavy metals from wastewaters. In this study, we isolated and identified different biofilm producing bacteria from wastewaters. We also characterized the biofilm matrix [i.e., extracellular polymeric substances (EPS)] produced by different bacteria. Out of 40 isolates from different wastewaters, only 11 (27.5%) isolates (static condition at 28°C) and 9 (22.5%) isolates (agitate and static conditions at 28 and 37°C) produced air–liquid (AL) and solid–air–liquid (SAL) biofilms, respectively, only on salt-optimized broth plus 2% glycerol (SOBG) but not in other media tested. Biomass biofilms and bacteria coupled with AL biofilms were significantly (P ≤ 0.001) varied in these isolates. Escherichia coli (isolate ENSD101 and ENST501), Enterobacter asburiae (ENSD102), Enterobacter ludwigii (ENSH201), Pseudomonas fluorescens (ENSH202 and ENSG304), uncultured Vitreoscilla sp. (ENSG301 and ENSG305), Acinetobacter lwoffii (ENSG302), Klebsiella pneumoniae (ENSG303), and Bacillus thuringiensis (ENSW401) were identified based on 16S rRNA gene sequencing. Scanning electron microscope (SEM) images revealed that biofilm matrix produced by E. asburiae ENSD102, uncultured Vitreoscilla sp. ENSG301, A. lwoffii ENSG302, and K. pneumoniae ENSG303 are highly fibrous, compact, and nicely interlinked as compared to the biofilm developed by E. ludwigii ENSH201 and B. thuringiensis ENSW401. X-ray diffraction (XRD) results indicated that biofilm matrix produced by E. asburiae ENSD102, uncultured Vitreoscilla sp. ENSG301, and A. lwoffii ENSG302 are non-crystalline amorphous nature. Fourier transform infrared (FTIR) spectroscopy showed that proteins and polysaccharides are the main components of the biofilms. Congo red binding results suggested that all these bacteria produced proteinaceous curli fimbriae and cellulose-rich polysaccharide. Production of cellulose was also confirmed by Calcofluor binding- and spectrophotometric assays. E. asburiae ENSD102, Vitreoscilla sp. ENSG301, and A. lwoffii ENSG302 were tested for their abilities to form the biofilms exposure to 0 to 2000 mg/L of copper sulfate (for Cu), zinc sulfate (for Zn), lead nitrate (for Pb), nickel chloride (for Ni), and potassium dichromate (for Cr), several concentrations of these metals activated the biofilm formation. The polysaccharides is known to sequester the heavy metals thus, these bacteria might be applied to remove the heavy metals from wastewater.

Environmental perturbations alter biochemical compounds in food crops. Arsenic (As), a toxic metalloid, is known to affect the cultivation of food crops in many regions of the world; however, the changes in chlorophyll, catalase (CAT), and proline in response to As stress and the role of stress relief substances remain largely unknown in mung bean (Vigna radiate L.). In this study, biochar (BC), arbuscular mycorrhizal fungi (AMF), and selenium (Se) were applied to soils as stress relief substances (under 30 mg kg^-1 As stress), and the effects of BC, AMF, and Se on chlorophyll a, chlorophyll b, total chlorophyll, CAT activity, and proline content were studied in different mung bean genotypes. Under As stress, the chlorophyll a, chlorophyll b, and total chlorophyll contents in BARI mung 3, BARI mung 5, and BARI mung 8 were found statistically similar. Meanwhile, CAT activity increased in comparison to the control due to the application of BC, AMF, and Se in mung bean crops. However, proline was found significantly lower in AMF, BC, and Se-treated mung bean. This indicates that oxidative stress was potentially minimized in As-stressed mung bean crops due to the application of these stress relief substances. Notably, AMF was relatively effective against As stress in comparison to BC and Se. It is concluded that BC, AMF, and Se are all highly effective in enhancing antioxidant defenses as well as the nutritional quality of mung bean crops under As stress.

Background: Anlotinib has been demonstrated in clinical trials to be effective in prolonging the progression-free survival (PFS) and overall survival (OS) of refractory advanced nonsmall cell lung cancer (NSCLC) patients. However, the underlying molecular mechanisms and predictive biomarkers of anlotinib are still unclear.Methods: A retrospective analysis of anlotinib administered to 294 NSCLC patients was performed to screen for underlying biomarkers of anlotinib-responsive patients. Transcriptome and functional assays were performed to understand the antitumour molecular mechanisms of anlotinib. Changes in serum CCL2 levels were analysed to examine the correlation of the anlotinib response between responders and nonresponders.Results: Anlotinib therapy was beneficial for prolonging OS in NSCLC patients harbouring positive driver gene mutations, especially patients harbouring the epithelial growth factor receptor (EGFR)^T790Mmutation. Moreover, anlotinib inhibited angiogenesis in an NCI-H1975-derived xenograft modelviainhibiting CCL2. Finally, anlotinib-induced serum CCL2 level decreases were associated with the benefits of PFS and OS in refractory advanced NSCLC patients.Conclusions: Our study reports a novel anti-angiogenesis mechanism of anlotinibviainhibiting CCL2 in an NCI-H1975-derived xenograft model and suggests that changes in serum CCL2 levels may be used to monitor and predict clinical outcomes in anlotinib-administered refractory advanced NSCLC patients using third-line therapy or beyond.