Inulin-type fructans used in formula have been shown to promote microbiota composition and stool consistency closer to those of breastfed infants and to have beneficial effects on fever occurrence, diarrhea, and incidence of infections requiring antibiotic treatment in infants. The primary study aim was to explore whether prophylactic supplementation with prebiotic fructans is able to influence the frequency of infectious diseases in kindergarten children during a winter period. A secondary objective was to ascertain the effect on the intestinal microbiota. 142 boys and 128 girls aged 3–6 y were randomly allocated to consume 6 g/d fructans or maltodextrin for 24 wk. At baseline, stool samples were collected for microbiota analysis and anthropometric measurements were made. During the intervention period diagnoses were recorded by physicians, whereas disease symptoms, kindergarten absenteeism, dietary habits, and stool consistency were recorded by parents. Baseline measurements were repeated at wk 24. In total 219 children finished the study. Both the relative abundance of Bifidobacterium (P < 0.001) and that of Lactobacillus (P = 0.014) were 19.9% and 7.8% higher, respectively, post data normalization, in stool samples of children receiving fructans as compared with those of controls at wk 24. This was accompanied by significantly softer stools within the normal range in the prebiotic group from wk 12 onwards. The incidence of febrile episodes requiring medical attention [0.65 ± 1.09 compared with 0.9 ± 1.11 infections/(24 wk × child), P = 0.04] and that of sinusitis (0.01 ± 0.1 compared with 0.06 ± 0.25, P = 0.03) were significantly lower in the prebiotic group. The number of infectious episodes and their duration reported by parents did not differ significantly between the 2 intervention groups. Prebiotic supplementation modified the composition of the intestinal microbiota and resulted in softer stools in kindergarten-aged children. The reduction in febrile episodes requiring medical attention supports the concept of further studies on prebiotics in young children. This trial was registered at clinicaltrials.gov as NCT03241355.

Although an extensive research is being undertaken, the ideal bone graft and evaluation method of the bone formation draw still a warranted attention. The purpose of this study was to develop a novel multimodal radiomics evaluation method, utilizing X-ray computed tomography (CT) and single photon emission computed tomography (SPECT) with Tc-99m-Methyl diphosphonate (Tc-99m-MDP) tracer. These modalities are intended to provide quantitative data concerning the mineral bone density (after evaluation it is referred to as opacity) and the osteoblast activity, at the same time. The properties of bone formation process within poly (methyl methacrylate)-based bone cement graft (PMMA) was compared to that of albumin coated, sterilized, antigen-extracted freeze-dried human bone grafts (HLBC), in caudal vertebrae (C5) of rats. The animals were scanned at 3 and 8 weeks after surgery. In both groups, the mean opacity increased, while the mean Tc-99m-MDP activity decreased. The later parameter was significant (n = 4, p = 0.002) only in HLBC group. The linear regression analysis of PMMA-treated group variables (mean opacity increase; mean Tc-99m-MDP activity decrease), revealed a negative correlation with the medium strength (r = 0.395, p = 0.605). Whereas, it showed strong positive correlation when HLBC group variables were analyzed (r = 0.772, p = 0.012). These results indicate that using HLBC grafts is advantageous in terms of the osteoblast activity and bone vascularization over PMMA cement. Using this regression analysis method, we were able to distinguish characteristics that otherwise could not be distinguished by a regular data analysis. Hence, we propose utilizing this novel method in preclinical tests, and in clinical monitoring of bone healing, in order to improve diagnosis of bone-related diseases.

Background: Medical students are at high risk for depressive symptoms, but few longitudinal studies have examined changes over time, especially among those repeating academic years. The aim of this study was to assess the prevalence and temporal trends of depressive symptoms and self-rated health among Hungarian medical students, examining the influence of demographic and lifestyle factors and the independent impact of academic repetition on mental health risk. Methods: In a four-year cohort study at the University of Pécs Medical School, students were classified as original-entry cohort (progressing according to the standard curriculum) or repeaters (those who began studies before 2016). Sample size was N = 196 in the first semester of the first year, N = 192 in the second semester of the first year, N = 157 in the second year, N = 147 in the third year, and N = 155 in the fourth year. Data were collected at five time points on lifestyle factors and depressive symptoms using the Beck Depression Inventory and a self-rated health questionnaire. Multivariate logistic regression identified risk and protective factors. Results: In the original-entry cohort, depressive symptoms peaked in the first two years (up to 52.6%), then declined to 24.6% by year four. Compared to age-matched peers, students had 3.1-fold higher odds of depressive symptoms at entry (OR = 3.10, CI: 2.007–4.773; p < 0.001), rising to 5.2-fold after the first exam period (OR = 5.20, CI: 3.375–8.000; p < 0.001). Among repeaters, odds of depressive symptoms remained over threefold higher than the general population (OR = 3.64, CI: 1.888–7.022, year IV/1), and self-rated health was significantly lower (p < 0.001). Financial hardship increased risk, while regular physical activity was protective. Conclusions: This study highlights the elevated and persistent mental health risks among repeaters and medical students with financial difficulties. Stratifying by academic progression reveals unique vulnerabilities, underscoring the need for targeted support in medical training.

Background/Objectives: Iodine deficiency remains an important global public health concern. Although iodized salt is the primary strategy for iodine deficiency prevention, its effectiveness depends on adequate iodine concentrations in commercially available products. However, laboratory data on the iodine content of retail salt products in Hungary are currently lacking. Therefore, this study aimed to determine the iodine concentration of commercially available iodized table salts in Hungary and to assess their compliance with the WHO-recommended range of 20–40 ppm. Methods: Twenty different brands of iodized table salt were purchased from major retail outlets in Pécs, Hungary, representing the dominant food retail sector. According to product labels, ten salts were fortified with potassium iodate (KIO₃) and ten with potassium iodide (KI). Iodine concentrations were determined by iodometric titration following WHO-recommended laboratory methods. All measurements were performed in triplicate and expressed as mean values. In addition, a small exploratory wholesale micro-survey examined purchasing patterns of iodized and non-iodized salt in the regional supply chain. Results: Measured iodine concentrations varied substantially among products, ranging from 0 to 33.9 ppm. Overall, 65% of the analyzed salt samples contained less than 20 ppm iodine, while only 35% fell within the WHO-recommended range of 20–40 ppm. Notably, several products declared iodine concentrations below recommended levels on their labels. The wholesale micro-survey showed that ten times more iodized than non-iodized salt was ordered during the observation period. Conclusions: These results suggest that the presence of iodized salt on the market does not necessarily guarantee adequate iodine supply and highlight the potential relevance of considering iodine status during the differential diagnosis of hypothyroidism.

Background: The ketogenic diet (KD) is one of the most widely followed dietary approaches worldwide, frequently promoted in popular media for weight loss and chronic disease management, although it also has established therapeutic applications in clinical medicine. However, our previous reviews have shown that existing clinical studies and meta-analyses on KD suffer from serious methodological limitations, raising concerns about their reliability. Considering this, the present study aimed to assess knowledge and opinions on KD among university students and academic staff. Methods: Cross-sectional, self-developed online questionnaire was distributed to 23,330 students and academic teachers at the University of Pécs, Hungary. Data was collected in October 2024. A total of 891 responses were included (710 students, 123 academic staff). Knowledge scores were calculated (maximum 17 points, including penalties for incorrect answers) and data were analyzed using descriptive statistics, ANOVA, chi-square tests, Pearson correlation, and logistic regression. Results: Only 7.3% of students and 13.5% of staff achieved ≥60% of the maximum knowledge score. Health-related faculties did not consistently outperform non–health-related ones; in fact, some non-health-related faculties achieved the highest mean scores. Completion of nutrition-related courses and reliance on PubMed were associated with higher knowledge, while current KD adherence among staff was negatively associated. Most participants (over 65%) were uncertain about the reliability of KD research. Conclusions: Knowledge of KD among both students and staff was limited, highlighting susceptibility to misinformation. Critical appraisal skills and reliable nutrition education are urgently needed at the population level to support disease prevention and to counterbalance misleading claims about KD.