Dr. Vladimir Brukhin

Embryology and Reproductive Biology, Komarov Botanical Institute of the Russian Academy of Sciences, Saint Petersburg, Sankt-Peterburg, Russia

Dr. Vladimir Brukhin

Embryology and Reproductive Biology, Komarov Botanical Institute of the Russian Academy of Sciences, Saint Petersburg, Sankt-Peterburg, Russia

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Academic Career Length

31Years (1996-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

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1 December 2007 in Cell

Journal Article

Genome-Wide High-Resolution Mapping of Exosome Substrates Reveals Hidden Features in the Arabidopsis Transcriptome

Julia Chekanova

Brian D. Gregory

Sergei V. Reverdatto

Huaming Chen

Ravi Kumar

Dmitry A. Belostotsky

10 December 2015 in Genome Biology

Journal Article

Genomic legacy of the African cheetah, Acinonyx jubatus

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Patterns of genetic and genomic variance are informative in inferring population history for human, model species and endangered populations. Here the genome sequence of wild-born African cheetahs reveals extreme genomic depletion in SNV incidence, SNV density, SNVs of coding genes, MHC class I and II genes, and mitochondrial DNA SNVs. Cheetah genomes are on average 95 % homozygous compared to the genomes of the outbred domestic cat (24.08 % homozygous), Virunga Mountain Gorilla (78.12 %), inbred Abyssinian cat (62.63 %), Tasmanian devil, domestic dog and other mammalian species. Demographic estimators impute two ancestral population bottlenecks: one >100,000 years ago coincident with cheetah migrations out of the Americas and into Eurasia and Africa, and a second 11,084–12,589 years ago in Africa coincident with late Pleistocene large mammal extinctions. MHC class I gene loss and dramatic reduction in functional diversity of MHC genes would explain why cheetahs ablate skin graft rejection among unrelated individuals. Significant excess of non-synonymous mutations in AKAP4 (p80 %) pleiomorphic sperm. The study provides an unprecedented genomic perspective for the rare cheetah, with potential relevance to the species’ natural history, physiological adaptations and unique reproductive disposition.

15 March 2003 in Sexual Plant Reproduction

Journal Article

Flower development schedule in tomato Lycopersicon esculentum cv. sweet cherry

Vladimir Brukhin

Michel Hernould

Nathalie Gonzalez

Christian Chevalier

Armand Mouras

14 January 2011 in The EMBO Journal

Journal Article

The Arabidopsis CUL4–DDB1 complex interacts with MSI1 and is required to maintain MEDEA parental imprinting

Eva Dumbliauskas

Esther Lechner

Miłosława Jaciubek

Alexandre Berr

Maghsoud Pazhouhandeh

Pascal Genschik

Protein ubiquitylation regulates a broad variety of biological processes in all eukaryotes. Recent work identified a novel class of cullin‐containing ubiquitin ligases (E3s) composed of CUL4, DDB1, and one WD40 protein, believed to act as a substrate receptor. Strikingly, CUL4‐based E3 ligases (CRL4s) have important functions at the chromatin level, including responses to DNA damage in metazoans and plants and, in fission yeast, in heterochromatin silencing. Among putative CRL4 receptors we identified MULTICOPY SUPPRESSOR OF IRA1 (MSI1), which belongs to an evolutionary conserved protein family. MSI1‐like proteins contribute to different protein complexes, including the epigenetic regulatory Polycomb repressive complex 2 (PRC2). Here, we provide evidence that Arabidopsis MSI1 physically interacts with DDB1A and is part of a multimeric protein complex including CUL4. CUL4 and DDB1 loss‐of‐function lead to embryo lethality. Interestingly, as in fis class mutants, cul4 mutants exhibit autonomous endosperm initiation and loss of parental imprinting of MEDEA, a target gene of the Arabidopsis PRC2 complex. In addition, after pollination both MEDEA transcript and protein accumulate in a cul4 mutant background. Overall, our work provides the first evidence of a physical and functional link between a CRL4 E3 ligase and a PRC2 complex, thus indicating a novel role of ubiquitylation in the repression of gene expression.

16 September 2005 in The Plant Cell

Journal Article

The RPN1 Subunit of the 26S Proteasome in Arabidopsis Is Essential for Embryogenesis

Vladimir Brukhin

Jacqueline Gheyselinck

Valeria Gagliardini

Pascal Genschik

Ueli Grossniklaus

The 26S proteasome plays a central role in the degradation of regulatory proteins involved in a variety of developmental processes. It consists of two multisubunit protein complexes: the proteolytic core protease and the regulatory particle (RP). The function of most RP subunits is poorly understood. Here, we describe mutants in the Arabidopsis thaliana RPN1 subunit, which is encoded by two paralogous genes, RPN1a and RPN1b. Disruption of RPN1a caused embryo lethality, while RPN1b mutants showed no obvious abnormal phenotype. Embryos homozygous for rpn1a arrested at the globular stage with defects in the formation of the embryonic root, the protoderm, and procambium. Cyclin B1 protein was not degraded in these embryos, consistent with cell division defects. Double mutant plants (rpn1a/RPN1a rpn1b/rpn1b) produced embryos with a phenotype indistinguishable from that of the rpn1a single mutant. Thus, despite their largely overlapping expression patterns in flowers and developing seeds, the two isoforms do not share redundant functions during gametogenesis and embryogenesis. However, complementation of the rpn1a mutation with the coding region of RPN1b expressed under the control of the RPN1a promoter indicates that the two RPN1 isoforms are functionally equivalent. Overall, our data indicate that RPN1 activity is essential during embryogenesis, where it might participate in the destruction of a specific set of protein substrates.

8 December 2017 in Heredity

Journal Article

Genome-wide genotyping uncovers genetic profiles and history of the Russian cattle breeds

Andrey A. Yurchenko

Nikolay Yudin

Ruslan Aitnazarov

Alexandra Plyusnina

Vladimir Brukhin

Denis Larkin

About Me

2024 – Visiting Fellow, School of Natural and Environmental Sciences, Newcastle University, UK 2015 – Leading Scientist, Part time. Plant Embryology and Reproductive Biology, Komarov Botanical Institute, Russian Academy of Sciences, St. Petersburg 2022 Fulbright VS, Laboratory of Molecular Systematics and Evolutionary Genetics, University of Florida, USA2020 – 2022 Professor Associate ITMO University, St. Petersburg. Head of Plant Genomics lab 2013 – 2020 Deputy Director and Acting Director of the Dobzhansky Center for Genome Bioinformatics at St. Petersburg State University 2011 – 2012 Research Fellow, University of Edinburgh, Institute Plant Molecular Science, UK2008 – 2011 Research Associate, IBERS University of Wales, UK 2006 - 2007 Research Associate Department of Plant Biology, University of Illinois USA2000 – 2006 Postdoctoral Department Plant Developmental Genetics, University of Zurich1999 - 2000 Postdoctoral Swedish University of Agricultural Sci., Uppsala, Sweden 1997 - 1999 Postdoctoral Cell Biology and Plant Biotechnology of Victor Segalen University Bordeaux 2 France1996 - 1997 March Internship Plant Cytology Wageningen Agricultural University 1994 Internship Plant Anatomy and Cytology of Lublin UMCS University, Poland1993 – 1997 Research fellow Embryology Komarov Botanical Institute 1990 – 1993 PhD Plant Embryology Botanical Institute, Russian Academy of Sciences1983 – 1988 Student of St.Petersburg State University, Plant Physiology and Biochemistry

Dr. Vladimir Brukhin

Dr. Vladimir Brukhin

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