This study investigates the effects of continuous and short-term direct current (DC) stimulation on the growth and nutrient composition of Nitzschia closterium f. minutissima, a marine diatom with significant potential in aquaculture feed. We explored the optimal conditions of DC stimulation by applying both short-term and continuous treatments at varying voltages (0 V, 1 V, 3 V, 5 V, 10 V for short-term; 0 V, 0.05 V, 0.5 V, 1 V for continuous). The results demonstrated that short-term DC stimulation significantly enhanced cell density and lipid accumulation, with maximum cell density increasing by 13.14% under 1 V stimulation compared to the control. However, continuous stimulation was less effective and showed growth inhibition in several cases. Nutrient analysis revealed that short-term stimulation, particularly at 3 V, led to a 67.66% increase in lipid content, while moderate continuous stimulation (0.5 V) showed a 39.47% increase in biomass dry weight. These findings suggest that short-term DC stimulation is a promising approach to optimize microalgal growth and nutrient accumulation for large-scale aquaculture production.