Antigens were conjugated on the surface of N-trimethylaminoethylmethacrylate chitosan (TMC) nanoparticles to induce systemic and mucosal immune responses after nasal immunization. TMC was synthesized by free radical polymerization and blank nanoparticles were prepared by ionic crosslinking of TMC and sodium tripolyphosphate. The model antigen (ovalbumin) was conjugated on the surface of blank nanoparticles (OVA-NP) through thioester bond formation. The cellular uptake of OVA-NP was investigated in Raw 264.7 macrophages and biodistribution of antigens was studied by the radioiodine labeling method. The immunological effects were evaluated by nasal administration of OVA-NP to Balb/C mice. The transport mechanism and nasal toxicity of OVA-NP were studied in rats. The cellular uptake of OVA-NP was significantly higher than that of ovalbumin-encapsulated nanoparticles (NPe) after 30 min. Nasally administered OVA-NP showed higher transport of antigens to cervical lymph nodes with higher targeting efficiency than all other groups. Compared with NPe, OVA-NP induced much higher levels of systemic and mucosal immune responses in Balb/C mice after three nasal immunizations. Ex vivo culturing of nasopharynx-associated lymphoid tissue (NALT) confirmed its participation in nasal immunization. The transport mechanism study revealed that OVA-NP can be transported across the nasal epithelium through glands and may be taken up in NALT through M cells. OVA-NP did not induce obvious toxicity to nasal mucosa or hemolysis in animals. The present study demonstrated that the conjugation of TMC nanoparticles with antigens is an effective strategy for nasal vaccination.

Self-localization and state estimation are crucial capabilities for agile drone autonomous navigation. This article presents a lightweight and drift-free vision-IMU-GNSS tightly coupled multisensor fusion (LDMF) strategy for drones’ autonomous and safe navigation. The drone is carried out with a front-facing camera to create visual geometric constraints and generate a 3D environmental map. Ulteriorly, a GNSS receiver with multiple constellations support is used to continuously provide pseudo-range, Doppler frequency shift and UTC time pulse signals to the drone navigation system. The proposed multisensor fusion strategy leverages the Kanade–Lucas algorithm to track multiple visual features in each input image. The local graph solution is bounded in a restricted sliding window, which can immensely predigest the computational complexity in factor graph optimization procedures. The drone navigation system can achieve camera-rate performance on a small companion computer. We thoroughly experimented with the LDMF system in both simulated and real-world environments, and the results demonstrate dramatic advantages over the state-of-the-art sensor fusion strategies.

To improve the accuracy of tracking the trunk center-of-mass (CoM) trajectory and foot-end trajectory in a fully electrically driven quadruped robot, an efficient and practical new trajectory tracking control method is designed. The proposed trajectory tracking method is mainly divided into trunk balance controller (TBC) and swing leg controller (SLC). In TBC, a quadruped robot dynamics model is developed to find the optimal foot-end force that follows the trunk CoM trajectory based on the model predictive control (MPC) principle. In SLC, the Bessel curve is planned as the desired trajectory at the foot-end, while the desired trajectory is tracked by a virtual spring-damping element driving the foot-end, meanwhile, the radial basis function neural network (RBFNN) is applied for supervisory control to improve the control performance for the system. The experimental results show that the control method can modify the robot’s foot-end trajectory tracking effect, so that the stability error can be eliminated and the robustness of the controller can be improved, meanwhile, the linear and circular trajectory for CoM can be tracked accurately and quickly.

Pose estimation and environmental perception are the fundamental capabilities of autonomous robots. In this paper, a novel real-time pose estimation and object detection (RPEOD) strategy for aerial robot target tracking is presented. The aerial robot is equipped with a binocular fisheye camera for pose estimation and a depth camera to capture the spatial position of the tracked target. The RPEOD system uses a sparse optical flow algorithm to track image corner features, and the local bundle adjustment is restricted in a sliding window. Ulteriorly, we proposed YZNet, a lightweight neural inference structure, and took it as the backbone in YOLOV5 (the state-of-the-art real-time object detector). The RPEOD system can dramatically reduce the computational complexity in reprojection error minimization and the neural network inference process; Thus, it can calculate real-time on the onboard computer carried by the aerial robot. The RPEOD system is evaluated using both simulated and real-world experiments, demonstrating clear advantages over state-of-the-art approaches, and is significantly more fast.

Geometric–semantic scene understanding is a spatial intelligence capability that is essential for robots to perceive and navigate the world. However, understanding a natural scene remains challenging for robots because of restricted sensors and time-varying situations. In contrast, humans and animals are able to form a complex neuromorphic concept of the scene they move in. This neuromorphic concept captures geometric and semantic aspects of the scenario and reconstructs the scene at multiple levels of abstraction. This article seeks to reduce the gap between robot and animal perception by proposing an ingenious scene-understanding approach that seamlessly captures geometric and semantic aspects in an unexplored environment. We proposed two types of biologically inspired environment perception methods, i.e., a set of elaborate biomimetic sensors and a brain-inspired parsing algorithm related to scene understanding, that enable robots to perceive their surroundings like bats. Our evaluations show that the proposed scene-understanding system achieves competitive performance in image semantic segmentation and volumetric–semantic scene reconstruction. Moreover, to verify the practicability of our proposed scene-understanding method, we also conducted real-world geometric–semantic scene reconstruction in an indoor environment with our self-developed drone.

This paper proposes a novel aerial manipulator with front cutting effector (AMFCE) to address the aerial physical interaction (APhI) problem. First, the system uncertainty and external disturbance during the system movement and contact operation are estimated by modeling the entire robot and contact position. Next, based on the established model, the nonlinear disturbance observer (NDO) is used to estimate and compensate the unknown external disturbance of the system and the uncertainty of the model parameters in real time. Then, the nonsingular terminal synovial membrane control method is used to suppress the part that is difficult to estimate. Finally, a controller which is suitable for the movement and operation of the entire system is designed. The controller’s performance is verified through experiments, and the results show that the design, modeling, and control of the entire system can achieve the APhI.