The burial of multiple individuals within a single funerary monument invites speculation about the relationships between the deceased: were they chosen on the basis of status, gender or relatedness, for example? Here, the authors present the results of aDNA and isotope analyses conducted on seven individuals from an Early Iron Age barrow at Dolge njive, south-eastern Slovenia. All seven individuals are close biological relatives. While the group composition suggests strict adherence to neither patrilineal nor matrilineal structures, the funerary tradition appears highly gendered, and family links through both the male and female lines seem important in structuring of the community. The results have implications for understanding of kinship and funerary practices in late prehistoric Europe.

To test the usefulness of the forensic PCR-MPS approach to eye and hair color prediction for aged skeletons, a customized version of the PCR-MPS HIrisPlex panel was used on two sets of samples. The first set contained 11 skeletons dated from the 3rd to the 18th centuries AD, and for each of them at least four bone types were analyzed (for a total of 47 samples). In the second set, 24 skeletons from the Second World War were analyzed, and only petrous bones from the skulls were tested. Good-quality libraries were achieved in 83.3% of the cases for the ancient skeletons and in all Second World War petrous bones, with 94.7% and 100% of the markers, respectively, suitable for SNP typing. Consensus typing was achieved for about 91.7% of the markers in 10 out of 11 ancient skeletons, and the HIrisPlex-S webtool was then used to generate phenotypic predictions. Full predictions were achieved for 3 (27.3%) ancient skeletons and 12 (50%) Second World War petrous bones. In the remaining cases, different levels of AUC (area under the receiver operating curve) loss were computed because of no available data (NA) for 8.3% of markers in ancient skeletons and 4.2% of markers in Second World War petrous bones. Although the PCR-based approach has been replaced with new techniques in ancient DNA studies, the results show that customized forensic technologies can be successfully applied to aged bone remains, highlighting the role of the template in the success of PCR-MPS analysis. However, because several typical errors of ancient DNA sequencing were scored, replicate tests and accurate evaluation by an expert remain indispensable tools.

ATR-FTIR spectroscopy provides detailed information about the physiochemical properties of the analysed material. It is used in archaeology and forensics for various purposes, including characterization of the preservation state of skeletal remains. Building on the previous research, we used an ATR-FTIR spectrometer to analyse samples from the remains at various PMIs (21st century AD – 16th century BC), deposition and storage conditions, and different skeletal elements. We used the spectra to assess the state of preservation of the remains and to test how informative the spectra are for estimating the PMI taking into account the skeletal element and the environmental conditions. The study of the spectra provided information on taphonomically induced physiochemical changes in the organic and inorganic components of the remains, which are related not only to the PMI but also to the conditions under which the remains were discovered or stored and, to a lesser extent, to the skeletal element. A clear separation between 21st and 20th century versus archaeological (≥18th century) samples was possible even in the case of significant taphonomically caused changes. In the older samples some overlaps remained because environmental factors overpowered time, nevertheless 80% of samples were placed in the correct century.