Populus euphratica, a key tree species in the ecologically fragile area of the Tarim River Basin, plays a crucial role in maintaining the ecological balance of the desert. In order to explore the response of Populus euphratica leaves to river water resources and drought climate change, the complex topological relationship between the leaf traits of Populus euphratica was comprehensively analyzed by establishing the leaf trait network of the mainstream of the Tarim River, and the central traits that play a key role in regulating the phenotype of Populus euphratica were identified. The adaptation strategies of Populus euphratica to different environments were clarified from the perspective of the overall leaf trait network. The results show that, with a decrease in river water flow, the internal coordination of the leaf traits weakened, forming a looser network structure. In the hyper-arid region, the leaf traits were more independent, the network was simpler, and it was easier for the leaf to coordinate the aggregation of specific traits. Leaf thickness and the related photosynthetic traits are the key to adaptive strategies, particularly the cuticle thickness and midvein vascular bundle area. Leaf anatomical traits are more critical than stoichiometry traits in drought resistance. Leaves tend to give priority to the relationship between structural traits. Populus euphratica leaves adjust their trait modularization through a trait substitution strategy to manage different drought conditions.

Leaves are the primary interface through which plants interact with the environment, their functional traits (morphology, anatomy, physiology) directly reflecting ecological strategies that mediate species-environment interactions. These traits link plant performance to ecosystem processes, shaping species distributions and coexistence via their complex relationships with climatic and edaphic factors. Based on previous work, we selected 20 P. euphratica trees along the desert riparian forest of the main stream of the Tarim River for leaf sample collection and habitat survey. We used 27 leaf traits of P. euphratica to visualize the leaf trait network (LTN). Through network structure parameters, such as edge density, diameter, average path length, and average clustering coefficient, the spatial pattern of the LTN and its relationships with 19 climatic factors and 11 soil factors were discussed using principal component analysis and correlation analysis. The results showed that: (1) there were significant differences in the parameters of the leaf trait network of P. euphratica along the main stream of the Tarim River. The variation coefficients of the diameter and average path length were the largest, respectively, whereas that of the average clustering coefficient was the smallest. (2) Among the parameters, only Modularity was significantly correlated with STK and SOM. But Average clustering coefficient was significantly positively correlated with Isothermality, Average path length and Diameter were significantly positively correlated with Min temperature of coldest month and the Average clustering coefficient was significantly negatively correlated with Min temperature of coldest month, the Diameter was significantly positively correlated with Precipitation of wettest month. In general, the correlation between climate factors and LTNs was stronger than soil factors. (3) The explanatory power of climatic factors alone on the leaf traits of P. euphratica was generally higher than that of soil factors, indicating that climatic conditions play a more decisive role in shaping the network structure of leaf traits of P. euphratica. However, the influence of soil conditions on some LTNs parameters cannot be ignored.The spatial variability of leaf trait networks is driven by climate and soil factors, with climate dominating along the Tarim River’s main course.

Drought stress triggers significant adaptive responses in plant morphology and physiology. As a representative xerophytic genus, Zygophyllum possesses distinct drought-adaptive traits in morphology, anatomical structure and physiological metabolism. This study examined the responses of three Zygophyllum species (Zygophyllum fabago, Zygophyllum brachypterum, and Zygophyllum obliquum) to progressive osmotic stress induced by 20% PEG-6000 over 28 days under controlled conditions. Among the three species, Z. brachypterum displayed the greatest tolerance, as evidenced by its lowest wilting rate, longest root elongation, and more stable leaf anatomical traits under stress, despite a general decrease in all measured structural parameters across species. Physiologically, Z. brachypterum showed the smallest reduction in leaf water content (5.99% vs. 13.51% in Z. fabago and 20.31% in Z. obliquum), the smallest increase in relative electrolyte leakage (126.72% vs. 172.84% and 162.91%, respectively), lower excised-leaf water loss, and sustained higher photosynthetic rates during later stress stages. It also accumulated the lowest levels of total reactive oxygen species (ROS) and malondialdehyde (MDA), alongside the highest proline content and the highest activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT). Based on a comprehensive evaluation integrating multiple morphological, anatomical, photosynthetic, antioxidant, and osmotic-adjustment indicators through principal component analysis and membership function analysis, the drought-resistance ranking at the seedling stage was determined as Z. brachypterum > Z. fabago > Z. obliquum. The superior tolerance of Z. brachypterum was associated with its more stable leaf structure, enhanced water retention, sustained late-stage photosynthesis, and stronger antioxidant and osmotic adjustment capacities. These findings provide physiological insights into PEG-induced osmotic stress tolerance in Zygophyllum seedlings.

The complex network relationship between leaf traits and their multiple traits is closely related to the environment, and is the result of interactions between various environmental factors such as climate and soil. Based on previous work, we selected 20 P. euphratica trees along the desert riparian forest of the main stream of the Tarim River for leaf sample collection and habitat survey. We used 27 leaf traits of P. euphratica to visualize the leaf trait network (LTN). Through network structure parameters, such as edge density, diameter, average path length, and average clustering coefficient, the spatial pattern of the LTN and its relationships with 11 soil factors and 19 climatic factors were discussed using principal component analysis and correlation analysis. The results showed that: (1) there were significant differences in the parameters of the leaf trait network of P. euphratica along the main stream of the Tarim River. The variation coefficients of the diameter and average path length were the largest, respectively, whereas that of the average clustering coefficient was the smallest. (2) There were both positive and negative correlations between each parameter and soil factors, such as soil water content, total potassium, and organic matter, and climatic factors, such as isothermality, minimum temperature of the coldest month, and precipitation of the wettest month. (3) The explanatory power of climate alone on the leaf traits of P. euphratica was generally higher than that of soil factors, indicating that climatic conditions play a more decisive role in shaping the network structure of leaf traits of P. euphratica. However, regarding the influence of network modularity, the contribution of soil factors was significant, indicating that the influence of soil conditions on some LTN parameters cannot be ignored.

The complex network relationship between leaf traits and their multiple traits is closely related to the environment, and is the result of interactions between various environmental factors such as climate and soil. Based on previous work, we selected 20 P. euphratica trees along the desert riparian forest of the main stream of the Tarim River for leaf sample collection and habitat survey. We used 27 leaf traits of P. euphratica to visualize the leaf trait network (LTN). Through network structure parameters, such as edge density, diameter, average path length, and average clustering coefficient, the spatial pattern of the LTN and its relationship with 11 soil factors and 19 climatic factors were discussed using principal component analysis and correlation analysis. The results showed that: (1) there were significant differences in the parameters of the leaf trait network of P. euphratica along the main stream of the Tarim River. The variation coefficients of the diameter and average path length were the largest, respectively, whereas that of the average clustering coefficient was the smallest. (2) There were both positive and negative correlations between each parameter and soil factors, such as soil water content, soil total potassium, and soil organic matter, and climatic factors, such as isothermality, minimum temperature of the coldest month, and precipitation of the wettest month. (3) The explanatory power of climate alone on the leaf traits of P. euphratica was generally higher than that of soil factors, indicating that climatic conditions play a more decisive role in shaping the network structure of leaf traits of P. euphratica. However, regarding the influence of network modularity, the contribution of soil factors was significant, indicating that the influence of soil conditions on some LTN parameters cannot be ignored.