A novel method using vitrified CRISPR/Cas12b reagent is developed for rapid, amplification-free genotyping of two single nucleotide polymorphisms (SNPs) in human SLCO1B1 genes. With optimized vitrification, the reagents can be stored at room temperature.
S. aureus is one of the most important bacteria associated with human diseases. UDP-N-acetylglucosamine 1-carboxyethylene transferase (MurA) is an importan