The effects of aqueous solutions applied as foliar spray and drench applications of glycerol were tested on the ‘Chantenay’ carrot ( Daucus carota L.) family Apiaceae, corn ( Zea mays L.) family Poaceae, and spearmint ( Mentha spicata L.) family Lamiaceae under greenhouse conditions. Foliar sprays and drenches were administered to carrots at concentrations of 0, 1, 3, 5, 10, 25, or 50 ml·L −1 . Fresh weights, dry weights, and taproot diameter from carrot seedlings sprayed with a solution containing 5 mL·L −1 (50 m m ) glycerol increased 105.6%, 158.4%, and 53.8%, respectively, when compared with untreated carrots. Foliar sprays were administered to corn at concentrations of 0, 0.1, 0.3, 0.5, and 1 ml·L −1 and spearmint at concentrations of 0, 1, 5, and 10 mL·L −1 . Growth responses increased in corn and spearmint by using certain glycerol concentrations. Fresh weights, dry weights, and shoot length from corn seedlings sprayed with a solution containing 0.5 mL·L −1 (5 m m ) glycerol increased 83.5%, 154.6%, and 90.9%, respectively, when compared with untreated corn. Fresh weights, dry weights, and shoot length from mint plants sprayed with a solution containing 5 mL·L −1 (50 m m ) glycerol increased 46.6%, 68.7%, and 102.5%, respectively, when compared with untreated plants. Glycerol applications can stimulate growth responses in diverse plant species.