A microbiological examination of the soils, polluted with different types of urban wastewater (Tashkent city), aiming determination of the microbial diversity and characterization of the bacterial community was carried out. The examination was conducted with use of classical microbiological methods with cultivation of samples on elective nutrient media. The soil sampling was carried out during winter season and period of plants’ vegetation. As result of examination the qualitative and quantitative characteristics of the bacterial community were determined and the microbial diversity was established. The predominant microorganisms of this community, capable to active functioning at chloride concentrations of the environment for up to 10 % and possessing high remediation potential towards biological and chemical pollutants, have been isolated. Rare strains belonging to the genus Amycolatopsis, which, in contrast to typical representatives of this genus, have the ability to form a water-soluble blue pigment, have been isolated. It was established that typical representatives of microbial biota, such as heterotrophic microorganisms Bacillus, Pseudomonas and actinomycetes, possess significant remediation potential towards biological and chemical pollution. It was determined that pollution of the soil caused by anthropogenic factors at the end of the day leads to decrease in species diversity and changes in composition of the soil microbiocenosis. The results obtained convincingly testify perspectives of biomonitoring and possible use of microorganisms in the processes of soil rehabilitation. The introduction of pollutant-resistant microorganisms, which are capable to degrade them, may become a practical approach for soil cleansing in the future.

From soils exposed to anthropogenic load, a bacterial strain resistant to pollutants was isolated and characterized. The strain was identified as Pseudomonas trivialis 381 based on morphological, cultural, physiological and biochemical characteristics, as well as MALDI-TOF and 16S rRNA analysis. Its sequence was submitted to the NCBI GenBank (OR234684.1). Studies were conducted to evaluate the influence of the strain on seed germination and plant growth qualities. Laboratory studies have shown the stimulating effect of P. trvialis 381 on tomato seeds; seeds treated with the bacterial cultural liquid increased the energy of germination and seed germination to 100% (with the control being 64% and 60%), and also led to an increase in the length of the stem and root, and the number of roots. Microvegetation experiments have shown the stimulating effect of P. trivialis 381 on the growth of tomatoes and rice, with the particularly noticeable development of the root system, which enabled plants to absorb nutrients more quickly and made them more resistant to adverse conditions. The cultivation of seeds treated with cultural liquid on artificially saline soils (by adding 10% NaCl) enhanced the plants’ resistance to salinity, resulting in better growth of primed plants and an increase in chlorophyll content to more than 3 times in the leaves.