Non-structural elements have been demonstrated to be essential for the dynamic performance of large-span structures. However, how to quantify their effect has not yet been fully understood. In this study, the contribution of non-structural elements to dynamic properties of large-span structures is systematically investigated via both field measurement and numerical simulation methods. Modal testing of an indoor stadium and an elevated highway bridge was conducted during different construction phases, and the corresponding modal characteristics were identified. Results show that the traditional capacity-based models are incapable of reflecting the actual dynamic characteristics of in-service structures since neglecting the effect of non-structural elements would result in remarkable discrepancies in modal properties. A general modeling framework incorporating the contribution of slab/deck pavement, infill walls (or crash barriers), and joints/connections for large-span structures is developed to quantitatively consider the effect of non-structural elements based on the principle of equivalence of stiffness and mass to the actual structure. The effectiveness of the method is validated by vibration measurement results.