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Gang Liang
Beijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Science, Beijing, China

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RCT
electrochemical impedance spectroscopy
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Adolescents
PCBs
congeners

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Research article
Published: 23 December 2020 in PLOS ONE
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Acetamiprid (ACE) is a kind of broad-spectrum pesticide that has potential health risk to human beings. Aptamers (Ap-DNA (1)) have a great potential as analytical tools for pesticide detection. In this work, a label-free electrochemical sensing assay for ACE determination is presented by electrochemical impedance spectroscopy (EIS). And the specific binding model between ACE and Ap-DNA (1) was further investigated for the first time. Circular dichroism (CD) spectroscopy and EIS demonstrated that the single strand AP-DNA (1) first formed a loosely secondary structure in Tris-HClO4 (20 mM, pH = 7.4), and then transformed into a more stable hairpin-like structure when incubated in binding buffer (B-buffer). The formed stem-loop bulge provides the specific capturing sites for ACE, forming ACE/AP-DNA (1) complex, and induced the RCT (charge transfer resistance) increase between the solution-based redox probe [Fe(CN)6]3−/4− and the electrode surface. The change of ΔRCT (charge transfer resistance change, ΔRCT = RCT(after)-RCT(before)) is positively related to the ACE level. As a result, the AP-DNA (1) biosensor showed a high sensitivity with the ACE concentration range spanning from 5 nM to 200 mM and a detection limit of 1 nM. The impedimetric AP-DNA (1) sensor also showed good selectivity to ACE over other selected pesticides and exhbited excellent performance in environmental water and orange juice samples analysis, with spiked recoveries in the range of 85.8% to 93.4% in lake water and 83.7% to 89.4% in orange juice. With good performance characteristics of practicality, sensitivity and selectivity, the AP-DNA (1) sensor holds a promising application for the on-site ACE detection.

ACS Style

Jianhui Zhen; Gang Liang; Ruichun Chen; Wenshen Jia. Label-free hairpin-like aptamer and EIS-based practical, biostable sensor for acetamiprid detection. PLOS ONE 2020, 15, e0244297 .

AMA Style

Jianhui Zhen, Gang Liang, Ruichun Chen, Wenshen Jia. Label-free hairpin-like aptamer and EIS-based practical, biostable sensor for acetamiprid detection. PLOS ONE. 2020; 15 (12):e0244297.

Chicago/Turabian Style

Jianhui Zhen; Gang Liang; Ruichun Chen; Wenshen Jia. 2020. "Label-free hairpin-like aptamer and EIS-based practical, biostable sensor for acetamiprid detection." PLOS ONE 15, no. 12: e0244297.

Journal article
Published: 29 March 2019 in Sensors
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In this study, the PEN3 electronic nose was used to detect and recognize fresh and moldy apples inoculated with Penicillium expansum and Aspergillus niger, taking Golden Delicious apples as the model subject. Firstly, the apples were divided into two groups: individual apple inoculated only with/without different molds (Group A) and mixed apples of inoculated apples with fresh apples (Group B). Then, the characteristic gas sensors of the PEN3 electronic nose that were most closely correlated with the flavor information of the moldy apples were optimized and determined to simplify the analysis process and improve the accuracy of the results. Four pattern recognition methods, including linear discriminant analysis (LDA), backpropagation neural network (BPNN), support vector machines (SVM), and radial basis function neural network (RBFNN), were applied to analyze the data obtained from the characteristic sensors, aiming at establishing the prediction model of the flavor information and fresh/moldy apples. The results showed that only the gas sensors of W1S, W2S, W5S, W1W, and W2W in the PEN3 electronic nose exhibited a strong signal response to the flavor information, indicating most were closely correlated with the characteristic flavor of apples and thus the data obtained from these characteristic sensors were used for modeling. The results of the four pattern recognition methods showed that BPNN had the best prediction performance for the training and testing sets for both Groups A and B, with prediction accuracies of 96.3% and 90.0% (Group A), 77.7% and 72.0% (Group B), respectively. Therefore, we demonstrate that the PEN3 electronic nose not only effectively detects and recognizes fresh and moldy apples, but also can distinguish apples inoculated with different molds.

ACS Style

Wenshen Jia; Gang Liang; Hui Tian; Jing Sun; Cihui Wan. Electronic Nose-Based Technique for Rapid Detection and Recognition of Moldy Apples. Sensors 2019, 19, 1526 .

AMA Style

Wenshen Jia, Gang Liang, Hui Tian, Jing Sun, Cihui Wan. Electronic Nose-Based Technique for Rapid Detection and Recognition of Moldy Apples. Sensors. 2019; 19 (7):1526.

Chicago/Turabian Style

Wenshen Jia; Gang Liang; Hui Tian; Jing Sun; Cihui Wan. 2019. "Electronic Nose-Based Technique for Rapid Detection and Recognition of Moldy Apples." Sensors 19, no. 7: 1526.

Journal article
Published: 13 March 2019 in International Journal of Environmental Research and Public Health
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Consumption of foodstuffs is the most likely route for human exposure to heavy metals. This study was designed to investigate the toxic metals (cadmium (Cd), lead (Pb), chromium (Cr), arsenic (As), and mercury (Hg)) concentrations in different foodstuffs (cereals, vegetables, fruits, fish, and meat) and then estimate the potential health risks of toxic metals via consumption to the local residents in Beijing, China. Most of the selected toxic metal levels in the foodstuffs were lower than the maximum allowable concentrations of Pb, Cr, Cd, As, and Hg for Chinese foodstuffs recommended in the China National Food Safety Standard. The health risks associated with the toxic metals Pb, Cr, Cd, As, and Hg were assessed based on the target hazard quotients (THQs) proposed by the United States Environmental Protection Agency (US EPA). The THQ values of the foodstuffs varied and were 0.03–0.29 for Cr, 0.02–0.23 for Pb, 0.01–0.33 for Cd, 0.01–0.06 for As, and 0.00–0.04 for Hg, not exceeding the maximum level of 1. The total THQ (TTHQ) values were 0.88 for vegetables, 0.57 for cereals, 0.46 for meat, 0.32 for fish, and 0.07 for fruits. This indicates that the risk contribution from vegetable intake (38.8%) was significant in comparison to that from other foodstuffs. The TTHQ values were 0.96 for Cr, 0.54 for Pb, 0.50 for Cd, 0.19 for As, and 0.09 for Hg, suggesting that Cr was a major risk contributor (41.7%) for the local residents of Beijing, which should attract great attention. However, the THQ/TTHQ values were all below 1, suggesting no health risks to the local population through consumption. Furthermore, dietary weekly intakes (WIs) were also calculated and the values were all lower than the proposed limit of Provisional Tolerable Weekly Intakes (PTWI) established by the the Food and Agriculture Organization of the United Nations (FAO) and the World Health Organization (WHO). This suggests no additional health risks as well as consistency with the THQ results.

ACS Style

Gang Liang; Wenwen Gong; Bingru Li; Jimin Zuo; Ligang Pan; Xinhui Liu. Analysis of Heavy Metals in Foodstuffs and an Assessment of the Health Risks to the General Public via Consumption in Beijing, China. International Journal of Environmental Research and Public Health 2019, 16, 909 .

AMA Style

Gang Liang, Wenwen Gong, Bingru Li, Jimin Zuo, Ligang Pan, Xinhui Liu. Analysis of Heavy Metals in Foodstuffs and an Assessment of the Health Risks to the General Public via Consumption in Beijing, China. International Journal of Environmental Research and Public Health. 2019; 16 (6):909.

Chicago/Turabian Style

Gang Liang; Wenwen Gong; Bingru Li; Jimin Zuo; Ligang Pan; Xinhui Liu. 2019. "Analysis of Heavy Metals in Foodstuffs and an Assessment of the Health Risks to the General Public via Consumption in Beijing, China." International Journal of Environmental Research and Public Health 16, no. 6: 909.

Journal article
Published: 01 February 2018 in Colloids and Surfaces A: Physicochemical and Engineering Aspects
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ACS Style

Wenwen Gong; Yanjun Yu; Gang Liang; Xinhui Liu; Baoshan Cui; Junhong Bai; Ping Han. Factor effects and mechanisms of the adsorption of Hg(II), Cd(II) and Ni(II) on charged liposomes. Colloids and Surfaces A: Physicochemical and Engineering Aspects 2018, 538, 460 -466.

AMA Style

Wenwen Gong, Yanjun Yu, Gang Liang, Xinhui Liu, Baoshan Cui, Junhong Bai, Ping Han. Factor effects and mechanisms of the adsorption of Hg(II), Cd(II) and Ni(II) on charged liposomes. Colloids and Surfaces A: Physicochemical and Engineering Aspects. 2018; 538 ():460-466.

Chicago/Turabian Style

Wenwen Gong; Yanjun Yu; Gang Liang; Xinhui Liu; Baoshan Cui; Junhong Bai; Ping Han. 2018. "Factor effects and mechanisms of the adsorption of Hg(II), Cd(II) and Ni(II) on charged liposomes." Colloids and Surfaces A: Physicochemical and Engineering Aspects 538, no. : 460-466.

Proceedings article
Published: 01 January 2018 in 2018 Detroit, Michigan July 29 - August 1, 2018
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ACS Style

Xinxin / Jin; Wenshen / Jia; Ligang / Pan; An / Li; Gang / Liang; Jieqiong / Dong. Risk estimation of pesticide residues in apple in Shanxi province in 2017. 2018 Detroit, Michigan July 29 - August 1, 2018 2018, 1 .

AMA Style

Xinxin / Jin, Wenshen / Jia, Ligang / Pan, An / Li, Gang / Liang, Jieqiong / Dong. Risk estimation of pesticide residues in apple in Shanxi province in 2017. 2018 Detroit, Michigan July 29 - August 1, 2018. 2018; ():1.

Chicago/Turabian Style

Xinxin / Jin; Wenshen / Jia; Ligang / Pan; An / Li; Gang / Liang; Jieqiong / Dong. 2018. "Risk estimation of pesticide residues in apple in Shanxi province in 2017." 2018 Detroit, Michigan July 29 - August 1, 2018 , no. : 1.

Original paper
Published: 16 December 2017 in Microchimica Acta
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A chemiluminescence (CL) based assay is described for the determination of the environmental pollutant 2-hydroxyfluorene (2-HOFlu) which is found to inhibit the CL of a system composed of the G-quadruplex/hemin complex (a DNAzyme), H2O2, and luminol. The G-rich aptamer PW17 is transformed to a potassium(I)-stabilized G-quadruplex-hemin complex which displays peroxidase-like activity to catalyze the oxidation of luminol by H2O2 which is accompanied by strong blue CL emission. On addition of 2-HOFlu, it will participate in the G-quadruplex DNAzyme-mediated oxidation by H2O2. As a result, CL intensity is decreased. The difference in CL intensity (ΔI) before and after addition of 2-HOFlu serves as the signal for its quantitation. In water of pH 9.0, a linear relationship is found for the 1 nM to 1 μM concentration range, with a 0.2 nM detection limit. The assay is highly selective over other fluorene derivatives. It was successfully applied to the determination of 2-HOFlu in spiked lake water samples. The method is rapid, cost-effective and convenient. Conceivably, it has a wide scope in that it may be applied to other target pollutants for which G-quadruplexes are available.

ACS Style

Gang Liang; Yan Man; An Li; Xinxin Jin; Ligang Pan; Xinhui Liu. Chemiluminescence assay for detection of 2-hydroxyfluorene using the G-quadruplex DNAzyme-H2O2-luminol system. Microchimica Acta 2017, 185, 54 .

AMA Style

Gang Liang, Yan Man, An Li, Xinxin Jin, Ligang Pan, Xinhui Liu. Chemiluminescence assay for detection of 2-hydroxyfluorene using the G-quadruplex DNAzyme-H2O2-luminol system. Microchimica Acta. 2017; 185 (1):54.

Chicago/Turabian Style

Gang Liang; Yan Man; An Li; Xinxin Jin; Ligang Pan; Xinhui Liu. 2017. "Chemiluminescence assay for detection of 2-hydroxyfluorene using the G-quadruplex DNAzyme-H2O2-luminol system." Microchimica Acta 185, no. 1: 54.

Review
Published: 14 October 2017 in Toxins
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Mycotoxins are one of the main factors impacting food safety. Mycotoxin contamination has threatened the health of humans and animals. Conventional methods for the detection of mycotoxins are gas chromatography (GC) or liquid chromatography (LC) coupled with mass spectrometry (MS), or enzyme-linked immunosorbent assay (ELISA). However, all these methods are time-consuming, require large-scale instruments and skilled technicians, and consume large amounts of hazardous regents and solvents. Interestingly, a microchip requires less sample consumption and short analysis time, and can realize the integration, miniaturization, and high-throughput detection of the samples. Hence, the application of a microchip for the detection of mycotoxins can make up for the deficiency of the conventional detection methods. This review focuses on the application of a microchip to detect mycotoxins in foods. The toxicities of mycotoxins and the materials of the microchip are firstly summarized in turn. Then the application of a microchip that integrates various kinds of detection methods (optical, electrochemical, photo-electrochemical, and label-free detection) to detect mycotoxins is reviewed in detail. Finally, challenges and future research directions in the development of a microchip to detect mycotoxins are previewed.

ACS Style

Yan Man; Gang Liang; An Li; Ligang Pan. Recent Advances in Mycotoxin Determination for Food Monitoring via Microchip. Toxins 2017, 9, 324 .

AMA Style

Yan Man, Gang Liang, An Li, Ligang Pan. Recent Advances in Mycotoxin Determination for Food Monitoring via Microchip. Toxins. 2017; 9 (10):324.

Chicago/Turabian Style

Yan Man; Gang Liang; An Li; Ligang Pan. 2017. "Recent Advances in Mycotoxin Determination for Food Monitoring via Microchip." Toxins 9, no. 10: 324.

Journal article
Published: 14 August 2017 in International Journal of Environmental Research and Public Health
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Human hair of different age groups and foodstuff samples were collected in Beijing, China. The concerned metals—Cd, Cr, Pb, As, and Hg—were analyzed, and the metal levels in relation to age, gender, and dietary intake were further assessed. Results showed the highest level of the metals was shown by Pb, with an average concentration of 1.557 ± 0.779 mg/kg, followed by Cr (0.782 ± 0.394), Hg (0.284 ± 0.094), As (0.127 ± 0.078), and Cd (0.071 ± 0.032), following a decreasing order of Pb > Cr > Hg > As > Cd, which were all below the upper limit of normal values in China. The heavy metal concentrations varied greatly among different age groups, and higher concentrations for Cd, Cr, Pb, and As appeared in female hair, whereas higher Hg concentration were found in male hair, suggesting that age and gender were not crucial factors for assessing metal concentrations in human hair. The ingestion of cereals and vegetables were the main route by which heavy metals in the environment create hazardous health effects for local inhabitants, but the estimated metal intakes through food consumption were all lower than the proposed limit of Provisional Tolerable Weekly Intake (PTWI), indicating that heavy metals posed no health risks for the inhabitants. Furthermore, little relationship was found between metal intakes and the corresponding metal levels in hair. Nevertheless, the results of this study can be used to analyze the internal heavy metal burden in the resident population of Beijing area and can also serve as reference for further studies.

ACS Style

Gang Liang; Ligang Pan; Xinhui Liu. Assessment of Typical Heavy Metals in Human Hair of Different Age Groups and Foodstuffs in Beijing, China. International Journal of Environmental Research and Public Health 2017, 14, 914 .

AMA Style

Gang Liang, Ligang Pan, Xinhui Liu. Assessment of Typical Heavy Metals in Human Hair of Different Age Groups and Foodstuffs in Beijing, China. International Journal of Environmental Research and Public Health. 2017; 14 (8):914.

Chicago/Turabian Style

Gang Liang; Ligang Pan; Xinhui Liu. 2017. "Assessment of Typical Heavy Metals in Human Hair of Different Age Groups and Foodstuffs in Beijing, China." International Journal of Environmental Research and Public Health 14, no. 8: 914.

Journal article
Published: 29 April 2017 in Toxins
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A rapid, portable, and semi-quantitative immunochromatographic strip was developed for rapid and visual detection of alternariol monomethyl ether (AME). For this purpose, the anti-AME monoclonal antibody (mAb) was prepared and identified. AME coupled to bovine serum albumin (BSA) via methyl 4-bromobutanoate was prepared as immunogen. The recoveries of AME in spiked cherry and orange fruits determined by competitive ELISA were 86.1% and 80.7%, respectively. A colloidal gold nanoparticle (CGN) and CGNs-mAb conjugate were synthesized, and on this basis, a competitive colloidal gold immunochromatographic strip was developed and applied to the detection of AME toxin in fruit samples. The intensity of red density of the test line (T line) is inversely proportional to AME concentration in the range 0.1–10 ng/mL. The visual limit of detection (LOD) of AME was found to be about 10 ng/mL. The semi-quantitative detection can be completed in 10 min. Moreover, the immunochromatographic strip has lower cross-reactivity with AME analogues, and it has a good stability performance (following 3 months of storage). Hence, the colloidal gold immunochromatographic strip could be used as a semi-quantitative tool for the on-site, rapid, and visual detection of AME in fruit.

ACS Style

Yan Man; Gang Liang; Fuchao Jia; An Li; Hailong Fu; Meng Wang; Ligang Pan. Development of an Immunochromatographic Strip Test for the Rapid Detection of Alternariol Monomethyl Ether in Fruit. Toxins 2017, 9, 152 .

AMA Style

Yan Man, Gang Liang, Fuchao Jia, An Li, Hailong Fu, Meng Wang, Ligang Pan. Development of an Immunochromatographic Strip Test for the Rapid Detection of Alternariol Monomethyl Ether in Fruit. Toxins. 2017; 9 (5):152.

Chicago/Turabian Style

Yan Man; Gang Liang; Fuchao Jia; An Li; Hailong Fu; Meng Wang; Ligang Pan. 2017. "Development of an Immunochromatographic Strip Test for the Rapid Detection of Alternariol Monomethyl Ether in Fruit." Toxins 9, no. 5: 152.

Review
Published: 01 March 2017 in Microchemical Journal
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ACS Style

Gang Liang; Yan Man; An Li; Xinxin Jin; Xinhui Liu; Ligang Pan. DNAzyme-based biosensor for detection of lead ion: A review. Microchemical Journal 2017, 131, 145 -153.

AMA Style

Gang Liang, Yan Man, An Li, Xinxin Jin, Xinhui Liu, Ligang Pan. DNAzyme-based biosensor for detection of lead ion: A review. Microchemical Journal. 2017; 131 ():145-153.

Chicago/Turabian Style

Gang Liang; Yan Man; An Li; Xinxin Jin; Xinhui Liu; Ligang Pan. 2017. "DNAzyme-based biosensor for detection of lead ion: A review." Microchemical Journal 131, no. : 145-153.

Journal article
Published: 01 July 2013 in Biosensors and Bioelectronics
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A highly sensitive, reusable G-rich DNA sensor was reported for the detection of α-naphthol by electrochemical impedance spectroscopy (EIS). Specifically, a single-stranded G-rich DNA was self-assembled on the electrode and transformed into K(+)-stabilized G-quadruplex, which could catalyze H2O2-mediated oxidation of α-naphthol (with hemin as a cofactor) to 1, 4-naphthoquinone precipitated on the DNA films. Due to the insolubility of 1, 4-naphthoquinone, the charge transfer resistance (RCT) was increased to maximum within 15 min. Depending on the difference in charge transfer resistance change (ΔRCT), the α-naphthol could be detected with the detection limit of 0.1 nM in Tris-ClO4 buffer solution (20mM, pH=7.4). Moreover, the sensor demonstrated a high selectivity over other selected phenolic compounds. The performance of the sensor in the real lake water was also explored with the detection limit of 0.8 nM. Finally, the regeneration of the sensor was investigated, which allowed for reuse more than 4 cycles with a mean recovery of 94% of the original signal.

ACS Style

Gang Liang; Xinhui Liu; Xiaohong Li. Highly sensitive detection of α-naphthol based on G-DNA modified gold electrode by electrochemical impedance spectroscopy. Biosensors and Bioelectronics 2013, 45, 46 -51.

AMA Style

Gang Liang, Xinhui Liu, Xiaohong Li. Highly sensitive detection of α-naphthol based on G-DNA modified gold electrode by electrochemical impedance spectroscopy. Biosensors and Bioelectronics. 2013; 45 ():46-51.

Chicago/Turabian Style

Gang Liang; Xinhui Liu; Xiaohong Li. 2013. "Highly sensitive detection of α-naphthol based on G-DNA modified gold electrode by electrochemical impedance spectroscopy." Biosensors and Bioelectronics 45, no. : 46-51.

Journal article
Published: 16 March 2012 in Analytical Methods
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An electrochemical DNA sensor based on DNA conformational changes for simultaneous detection of Hg2+ and Pb2+ was reported. The sensor was consisted of a probe strand (DNA), a Pb2+-specific DNAzyme, and a substrate strand contains mercury-specific oligonucleotide (MSO). When Hg2+ and Pb2+ interacted with DNA, the induced conformational changes were tracked by electrochemical impedance spectroscopy (EIS), which led to a decreased RCT. The RCT difference (ΔRCT) was applied to selectively detect Hg2+ and Pb2+ with detection limit of 1 pM and 0.1 pM, respectively. Through using masking agents, such as cysteine (masking Hg2+) and G-DNA (CTG-GGA-GGG-AGG-GAG-GGA) (masking Pb2+), Hg2+ and Pb2+ were simultaneously detected in buffer solution, human serum and river water, respectively.

ACS Style

Liu Shi; Gang Liang; Xiaohong Li; Xinhui Liu. Impedimetric DNA sensor for detection of Hg2+ and Pb2+. Analytical Methods 2012, 4, 1036 .

AMA Style

Liu Shi, Gang Liang, Xiaohong Li, Xinhui Liu. Impedimetric DNA sensor for detection of Hg2+ and Pb2+. Analytical Methods. 2012; 4 (4):1036.

Chicago/Turabian Style

Liu Shi; Gang Liang; Xiaohong Li; Xinhui Liu. 2012. "Impedimetric DNA sensor for detection of Hg2+ and Pb2+." Analytical Methods 4, no. 4: 1036.

English abstract
Published: 01 October 2011 in Huan jing ke xue= Huanjing kexue
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ACS Style

Li Zhang; Yang Chen; Xin-Hui Liu; Dian-Dou Xu; Ling-Ling Ma; Zhi-Hong Shi; Gang Liang; Liu Shi; Hong Ouyang. [Levels of polychlorinated biphenyls in adolescents' hair from Gongzhuling, Jilin]. Huan jing ke xue= Huanjing kexue 2011, 32, 1 .

AMA Style

Li Zhang, Yang Chen, Xin-Hui Liu, Dian-Dou Xu, Ling-Ling Ma, Zhi-Hong Shi, Gang Liang, Liu Shi, Hong Ouyang. [Levels of polychlorinated biphenyls in adolescents' hair from Gongzhuling, Jilin]. Huan jing ke xue= Huanjing kexue. 2011; 32 (10):1.

Chicago/Turabian Style

Li Zhang; Yang Chen; Xin-Hui Liu; Dian-Dou Xu; Ling-Ling Ma; Zhi-Hong Shi; Gang Liang; Liu Shi; Hong Ouyang. 2011. "[Levels of polychlorinated biphenyls in adolescents' hair from Gongzhuling, Jilin]." Huan jing ke xue= Huanjing kexue 32, no. 10: 1.