Vibrio vulnificus is a free-living marine bacterium associated with the contamination of fish and shellfish—the most consumed seafood in Asia. Owing to its potentially lethal clinical consequences, the consumption of seafood contaminated with V. vulnificus has become a growing public health concern. This systematic review with meta-analysis and meta-regression aimed to integrate data on the prevalence of seafood-borne V. vulnificus specifically in Asia and assess the potential risk factors that can influence the outcomes. A comprehensive literature search of four electronic databases yielded 279 relevant studies, among which 38 fulfilled the inclusion criteria. These selected studies were subjected to risk-of-bias assessment and data extraction by three independent researchers. A meta-analysis of the eligible studies estimated the overall prevalence of seafood-borne V. vulnificus in Asia to be 10.47% [95% confidence interval (CI): 6.8–15.8%], with bivalve shellfish, such as oysters, mussels, clams, and cockles being the most contaminated seafood. The highest prevalence was reported in Japan, where 47.6% of the seafood samples tested positive for V. vulnificus. The subgroup and meta-regression analyses identified three potential covariates—detection method, publication year, and country—associated with between-study heterogeneity. Furthermore, data visualization displayed the variations in V. vulnificus prevalence across the studies, associated with differences in sample type, sample size, and sampling stage. This study provides valuable insights into the prevalence of V. vulnificus in fish and shellfish across the entire Asian continent and highlights the potential factors that cause variation in the prevalence rates among the studies. These findings underscore the importance of enhancing hygiene measures throughout the seafood supply chain to mitigate V. vulnificus infection risks and ensure the safety of consumers.

The escalating antimicrobial resistance (AMR) in highly virulent Vibrio vulnificus poses a significant public health concern in Asia. Profiling the antibiogram of this pathogen is crucial for revealing its complex AMR patterns and guiding the selection of appropriate medications. Although previous studies have provided valuable insights regarding V. vulnificus AMR, they are constrained by limited sample diversity, inconsistent methodologies, and insufficient regional data. Moreover, no systematic attempt has been made to synthesize V. vulnificus AMR data across various sources and regions in Asia. A systematic review and meta-analysis are thus conducted in this study to assess the current AMR status of V. vulnificus isolated from clinical, environmental, and seafood samples. By synthesizing data from 32 articles across 13 Asian countries, a broader antibiogram has been provided, covering 13 major antimicrobial groups against V. vulnificus. Subgroup and regression analyses were also performed using study-level and country-specific covariates to explore the associated risk factors. The findings revealed low AMR rates for tetracyclines (4.89 %), quinolones (1.85 %), nitrofurans (0.86 %), and phenicols (0.61 %), highlighting their potential as primary treatment options. Conversely, high AMR rates were detected for lincosamides (80.32 %), polypeptides (64.42 %), and glycopeptides (56.14 %), necessitating careful consideration for their clinical use. For study-level covariates, subgroup and meta-regression analyses revealed that variations in the type of antimicrobial (R ² = 26.5 %, p < 0.0001), country (R ² = 18.33 %, p < 0.0001), and pathogen source (R ² = 10.46 %, p = 0.0007) significantly contributed to between-study heterogeneity in the detected AMR rates across studies. Moreover, the analyses of country-specific covariates indicated that antimicrobial consumption (AMC) in healthcare systems (R ² = 29.3, p = 0.06) and the country's gross domestic product (GDP) (R ² = 28.59, p = 0.06) affected the variations in AMR rates across countries to some extent. Consideration of study-level and country-specific covariates is thus recommended for future research to effectively mitigate the threat of V. vulnificus AMR across Asia and reduce its pervasive impact on public health.

Bacterial skin infections, particularly pyoderma and otitis externa, are widespread in dogs, primarily caused by Staphylococcus and Pseudomonas species. This study evaluates the prevalence and types of bacterial pathogens in affected dogs in South Korea using a meta-analytical approach. Following the PRISMA guidelines, five electronic databases were searched for relevant studies published between 1990 and 2024. Three researchers independently performed data extraction and quality assessment. A subgroup analysis explored the variability in pathogen prevalence across studies based on bacterial genus, bacterial species, publication year, sampling year, sampling location, infection type, diagnostic method, and sample size. Publication bias was evaluated using funnel plots and Egger’s regression test, with all analyses conducted using the R program. Of the 944 articles, 29 met the eligibility criteria. The pooled bacterial prevalence among infected dogs was 99.95% (95%CI: 99.85–100). Staphylococcus was the most prevalent genus (95.93%), followed by Pseudomonas (48.43%), Enterococcus (20.32%), and Escherichia (17.63%). The most common species were Staphylococcus pseudintermedius (78.89%), Staphylococcus intermedius (71.43%), and Pseudomonas aeruginosa (46.13%). This study underscores the need for comprehensive treatment strategies targeting Gram-negative and Gram-positive bacteria, emphasizing further research on antimicrobial resistance patterns and treatment efficacy to enhance canine health outcomes in South Korea.

Hossain FM, et al. Immune Netw. 2021 Aug;21(4):e26. https://doi.org/10.4110/in.2021.21.e26