Dr. Bipin D Lade
The National Center for Earth and Environmental Nanotechnology Infrastructure, Virginia Tech, Blacksburg, Virginia, USA
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Academic Career Length
13Years (2014-Present)
(Calculated once Scilit publication data is synced: current year − year of first publication + 1)
Representative Publications
View All Publications26 November 2020
Book Chapter
Bipin D LadeASArti S. Shanware
Arti S. Shanware
The greener way of producing silver nanoparticles is the easiest, cheapest and most efficient way of producing large-scale nanoparticles that have no adverse effect on the environment. The nanosynthesis using various methodologies and the biological synthesis of silver nanoparticles have been discussed in detail. Plant extracts have been known to be competent for the extracellular biosynthesis of silver nanoparticles suggested by the various publications. Further, effects of various sources and methods on nanoparticle synthesis have been examined. Additionally, the impact of conditions such as dark, light, heating, boiling, sonication, autoclave on the size and shape of colloidal nanoparticles has been analyzed. Moreover, effects of specific parameters such as leaf extract concentration, AgNO3, reaction temperature, pH, light and stirring time for nanoparticle synthesis are discussed, and the impact of silver nanoparticles on plant physiology has examined.
1 January 2019
Book Chapter
Bipin D LadeDGDayanand P. Gogle
Dayanand P. Gogle
DLDiksha B. Lade
Diksha B. Lade
GMGautam M. Moon
Gautam M. Moon
SNSukhdev B. Nandeshwar
Sukhdev B. Nandeshwar
SKShravan D. Kumbhare
Shravan D. Kumbhare
26 October 2017
Journal Article
Bipin D LadeDGDayanand P Gogle
Dayanand P Gogle
SNSukhdev B Nandeshwar
Sukhdev B Nandeshwar
1 April 2017 in Applied Nanoscience
Journal Article
Bipin D LadeAPAnita S. Patil
Anita S. PatilThe main purpose of the experiment is to develop a greener low cost SNP fabrication steps using factories of secondary metabolites from Passiflora leaf extract. Here, the leaf extraction process is omitted, and instead a leaf disc was used for stable SNP fabricated by optimizing parameters such as a circular leaf disc of 2 cm (1, 2, 3, 4, 5) instead of leaf extract and grade of pH (7, 8, 9, 11). The SNP synthesis reaction is tried under room temperature, sun, UV and dark condition. The leaf disc preparation steps are also discussed in details. The SNP obtained using (1 mM: 100 ml AgNO3+ singular leaf disc: pH 9, 11) is applied against featured room temperature and sun condition. The UV spectroscopic analysis confirms that sun rays synthesized SNP yields stable nano particles. The FTIR analysis confirms a large number of functional groups such as alkanes, alkyne, amines, aliphatic amine, carboxylic acid; nitro-compound, alcohol, saturated aldehyde and phenols involved in reduction of silver salt to zero valent ions. The leaf disc mediated synthesis of silver nanoparticles, minimizes leaf extract preparation step and eligible for stable SNP synthesis. The methods sun and room temperature based nano particles synthesized within 10 min would be use certainly for antimicrobial activity.
23 August 2014 in SpringerPlus
Journal Article
Bipin D LadeAPAnita Surendra Patil
Anita Surendra PatilHPHariprassad Madhukarrao Paikrao
Hariprassad Madhukarrao PaikraoIn Present work, the main objective is to develop less time consuming protocol for genomic DNA isolation from leaves of Passiflora foetida. Optimized protocol is cost effective, as it avoided use of expensive liquid nitrogen. The important parameters of CTAB buffer composition such as Polyvinylpyrrolidone PVP40000 (without PVP, 1%, 2%, 3.5%, 4.0%, 4.5%, 5.0%), CTAB (w, 1%, 2%, 3%, 4%, 5%), water bath temperature (30°C to 70°C) and duration on water bath for half hr and one and half hr has been optimized. CTAB (2%), PVP (1%), water bath temperature (70%), duration on water bath (1 hr) has efficiently yielded DNA quality of 200-1782 μg/0.5gm from leaf, stem, root, tendril and flower. However, 168 μg - 1782 μg of DNA has been obtained from 0.5 g of leaf of Passiflora foetida. Polyphenol contamination has been overcome using 5M NaCl and PVP. Acetate has been used for obtaining double-stranded DNA in stabilized form. Current DNA extraction protocol takes maximum of four hours for completion, which is many time savings. RAPD-PCR reaction parameters such as DNA concentration (100ng), Primer concentration (2 μM), Dream Taq polymerase (2 U), annealing temperature (29°C) and number of cycles for amplification of DNA has been optimized. Primer fragment Akansha 7 shows high polymorphism of 7 fragments ranges from 200bp – 2500 bp. Current optimized protocol of DNA isolation is specifically for Passiflora foetida, which can be used for downstream molecular techniques.
About Me
Bipin D. Lade is a Research Associate in the Department of Geosciences at Virginia Tech and is affiliated with the National Center for Earth and Environmental Nanotechnology Infrastructure (NanoEarth). His research lies at the intersection of environmental science, nanoscience, and materials science, with an emphasis on understanding and addressing micro- and nanoscale particles in environmental systems.
His research focuses on the occurrence, characterization, behavior, and remediation of microplastics and nanoplastics in aquatic environments. He combines environmental sampling and sample preparation with analytical approaches, including micro-Fourier transform infrared (µFTIR) spectroscopy, scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM-EDS), dynamic light scattering (DLS), and fluorescence microscopy.
A major focus of his current research is the development of food-grade hydrophobic polymer (FGHP)-based technologies for microplastic removal from water. This work has resulted in a U.S. provisional patent application and ongoing technology development through Virginia Tech Intellectual Properties (VTIP). He also develops depth-targeted environmental sampling approaches, including autonomous underwater vehicle (AUV)-based filtration and spectroscopic characterization of plastic particles.
Lade has authored more than 17 peer-reviewed publications and 7 book chapters, with more than 544 citations. He serves on the editorial boards of the Internat