Objective: To investigate the feasibility and efficacy of carrying out pediatric laparoscopic partial cystectomies (LPC) when treating benign bladder tumors and urachal cysts.Methods: Retrospectivey analyzing 4 clinical cases involving children with bladder tumors, which were collected from October 2017 to December 2018. In these clinical cases, there were 3 male children and 1 female child, aged from 4.5 to 9.4 years old, with an average age of 6.5 years. An intraperitoneal laparoscopic partial cystectomy was performed in the treatment of 3 of these patients with benign bladder tumors and in 1 patient with an urachal cyst. The surgical procedures included a partial cystectomy and a complete intracavitary bladder suture.Results: All 4 cases were successful and no operation was transferred to opensurgery. The operation time was 100–120 min, with an average time of 108 min. The intraoperative blood loss was 10–20 ml, with an average loss of 15 ml. 6 h after the operation, the patients still maintained a fluid diet and 1 case of hematuria had occurred, with the catheter removed 12 days after the operation. No postoperative urine leakage, intestinal adhesion or intestinal obstruction occurred, and the average postoperative hospitalization time was 14 days.Conclusion: Laparoscopic partial cystectomy is a safe and feasible method to be used for the treatment of benign bladder tumors and urachal cysts. It presents the advantages of being minimally invasive, and having a quick recovery and short hospitalization time. It is an alternative surgical method for the treatment of pediatric benign bladder tumors.

Background: TIR domain containing adaptor molecule 1 (TICAM1) is a coding gene participating in immune and inflammation responses to malignant cells. However, the role of TICAM1 in Wilms tumor (WT) is rarely known. Materials and methods: The expression level of TICAM1 was calculated in the WT TARGET cohort and validated using the GSE66405 cohort. The Kaplan–Meier method was employed to investigate the potential clinical value of TICAM1 and the association between its expression level and clinical features. The influence of TICAM1 on immune infiltration was examined by ESTIMATE, CIBERSORT and MCPcounter algorithms. IC50 of chemotherapeutic drugs was calculated by “pRRophetic” R package. Results: TICAM1 was downregulated in WT patients with worse prognosis and a more advanced clinical stage. Moreover, a low expression level of TICAM1 contributed to less immune cell infiltration, few protective immune cells and more antitumor immune cells. Conclusions: TICAM1 exerts a significant impact on the prognosis, progression and immune infiltration condition of WT.

Background: To explore the potential role of m6A methylation modification in Wilms Tumor (WT) by m6A-RNA Methylation (m6A) regulators. Methodology: The association of m6A modification patterns with immune and prognostic characteristics of tumors was systematically evaluated using 19 m6A regulators extracted from Wilms Tumor’s samples in public databases. A comprehensive model of “m6Ascore” was constructed using principal component analysis, and its prognostic value was evaluated. Results: Almost all m6A regulators were differentially expressed between WT and normal tissues. Unsupervised clustering identified three distinct m6A clusters that differed in both immune cell infiltration and biological pathways. The m6Ascore was constructed to quantify m6A modifications in individual patients. Our analysis suggests that m6Ascore is an independent prognostic factor for WT and can be used as a novel predictor of WT prognosis. Conclusions: This study comprehensively explored and systematically characterized m6A modifications in WT. m6A modification patterns play a critical role in the tumor immune microenvironment (TIME) and WT prognosis. m6Ascore provides a more comprehensive understanding of m6A modifications in WT and offers a practical tool for predicting WT prognosis. This study will help clinicians to identify valid indicators of WT to improve the poor prognosis of this disease. Supplementary Information: The online version contains supplementary material available at https://www.aliyundrive.com/drive/folder/64be739cd6956a741fb24670baeea53422be6024.

In the field of medical education, the innovation and optimization of teaching methods have always been the key to improving the quality of education. The popularization of the WeChat platform has provided a broader application space for question banks, especially digital question banks. To improve the teaching quality of pediatric surgery, a digital question bank for pediatric surgery was constructed based on the WeChat platform and applied to the clinical teaching of pediatric surgery. We explore whether integrating the digital question bank into the WeChat platform can help improve examination scores and also explore students' satisfaction. In this study, a clinical question bank covering pediatric surgery was established and then entered into the WeChat Official Accounts Platform. The instructor guides the medical students to use the WeChat platform for daily teaching activities and to complete the assessment of the digital question bank. A relevant evaluation system was established based on the big data results provided by the WeChat Official Account. This evaluation system was composed of the evaluation of instructors for the learning initiative and knowledge mastery of medical students and the evaluation of medical students for the teaching level of instructors and the digital question bank pattern. A digital question bank of pediatric surgery was successfully established. A total of about 3,200 questions in the question bank were uploaded to the WeChat Official Account of the pediatric surgery department of Qilu Hospital of Shandong University. Based on the digital question bank, partial clinical teaching tasks of pediatric surgery were completed, and the learning effects of medical students were evaluated. The question bank exhibited a favorable difficulty coefficient and discrimination, and the examination scores conformed to the normal distribution, indicating the higher scientific and effectiveness of this question bank. The questionnaire survey results demonstrated that most medical students maintained that the application of the new question bank based on the WeChat Official Accounts Platform can increase their learning interest in pediatrics, cultivate their clinical thinking, and enhance their clinical ability. A digital question bank of pediatric surgery was established for the first time based on the WeChat Official Accounts Platform. This digital question bank can be employed to evaluate the learning effects of medical students more scientifically, which contributes to improving teaching quality and teaching management. Overall, the new pediatric surgery question bank and the WeChat Official Accounts Platform can satisfy the development demands of pediatric surgery in the new era, exhibiting broad application prospects.