Leguminous plants form root nodules with rhizobia that fix atmospheric dinitrogen (N₂) for the nitrogen (N) nutrient. Combined nitrogen sources, particular nitrate, severely repress nodule growth and nitrogen fixation activity in soybeans (Glycine max [L.] Merr.). A microarray-based transcriptome analysis and the metabolome analysis were carried out for the roots and nodules of hydroponically grown soybean plants treated with 5 mM of nitrate for 24 h and compared with control without nitrate. Gene expression ratios of nitrate vs. the control were highly enhanced for those probesets related to nitrate transport and assimilation and carbon metabolism in the roots, but much less so in the nodules, except for the nitrate transport and asparagine synthetase. From the metabolome analysis, the concentration ratios of metabolites for the nitrate treatment vs. the control indicated that most of the amino acids, phosphorous-compounds and organic acids in roots were increased about twofold in the roots, whereas in the nodules most of the concentrations of the amino acids, P-compounds and organic acids were decreased while asparagine increased exceptionally. These results may support the hypothesis that nitrate primarily promotes nitrogen and carbon metabolism in the roots, but mainly represses this metabolism in the nodules.

Soybean (Glycine max (L.) Merr.) plants form root nodules and fix atmospheric dinitrogen, while also utilizing the combined nitrogen absorbed from roots. In this study, nodulated soybean plants were supplied with 5 mM N nitrate, ammonium, or urea for 3 days, and the changes in metabolite concentrations in the xylem sap and each organ were analyzed. The ureide concentration in the xylem sap was the highest in the control plants that were supplied with an N-free nutrient solution, but nitrate and asparagine were the principal compounds in the xylem sap with nitrate treatment. The metabolite concentrations in both the xylem sap and each organ were similar between the ammonium and urea treatments. Considerable amounts of urea were present in the xylem sap and all the organs among all the treatments. Positive correlations were observed between the ureides and urea concentrations in the xylem sap as well as in the roots and leaves, although no correlations were observed between the urea and arginine concentrations, suggesting that urea may have originated from ureide degradation in soybean plants, possibly in the roots. This is the first finding of the possibility of ureide degradation to urea in the underground organs of soybean plants.

N is the most crucial nutrient for plant growth and yield. Soybeans require a large amount of N for growth and seed production because of their high protein content. Soybean plants fix N₂ by root nodules in association with soil bacteria, rhizobia, but both the fixed N and the N absorbed from roots are essential to obtain a maximum seed yield. However, excess or inappropriate N fertilizer application represses N₂ fixation and reduces seed yield. A basal deep placement of lime nitrogen promoted soybean seed yield without inhibiting N₂ fixation activity in Japan. This study aimed to evaluate whether this technology can be applied in the Far East of Russia. The effects of deep placement of lime N with a wide row (75 cm) on the growth and seed yield of four Russian varieties were investigated. Without N fertilization, the average seed yield in wide rows was 2.77 t/ha, which was not significantly different from that in narrow rows (2.39 t/ha). Deep placement of lime nitrogen with wide rows increased total mechanical seed yield by 38%, 53%, 17%, and 6% in Primorskaya 4, 13, 81, and 86, respectively. The effect of basal urea application in narrow rows varied among cultivars. Soil analysis and the N composition in xylem sap indicated that the Russian field is richer in soil N than that in Niigata, and the contribution of N derived from N₂ fixation was lower than that in Niigata. The effects of row spacing and N fertilization on seed yield varied by variety; therefore, it is necessary to evaluate each variety to determine the optimal row spacing and N fertilization. The field experiment indicated that the deep placement of lime N promoted seed yield of Russian cultivars. This technique may be applied in soybean cultivation in a large field if the appropriate machine is available.