Ataxic gait monitoring and assessment of neurological disorders belong to important multidisciplinary areas that are supported by digital signal processing methods and machine learning tools. This paper presents the possibility of using accelerometric data to optimise deep learning convolutional neural network systems to distinguish between ataxic and normal gait. The experimental dataset includes 860 signal segments of 16 ataxic patients and 19 individuals from the control set with the mean age of 38.6 and 39.6 years, respectively. The proposed methodology is based upon the analysis of frequency components of accelerometric signals simultaneously recorded at specific body positions with a sampling frequency of 60 Hz. The deep learning system uses all of the frequency components in a range of (0,30) Hz. Our classification results are compared with those obtained by standard methods, which include the support vector machine, Bayesian methods, and the two-layer neural network with features estimated as the relative power in selected frequency bands. Our results show that the appropriate selection of sensor positions can increase the accuracy from 81.2% for the foot position to 91.7% for the spine position. Combining the input data and the deep learning methodology with five layers increased the accuracy to 95.8%. Our methodology suggests that artificial intelligence methods and deep learning are efficient methods in the assessment of motion disorders and they have a wide range of further applications.

Multimodal signal analysis based on sophisticated sensors, efficient communication systems and fast parallel processing methods has a rapidly increasing range of multidisciplinary applications. The present paper is devoted to pattern recognition, machine learning, and the analysis of sleep stages in the detection of sleep disorders using polysomnography (PSG) data, including electroencephalography (EEG), breathing (Flow), and electro-oculogram (EOG) signals. The proposed method is based on the classification of selected features by a neural network system with sigmoidal and softmax transfer functions using Bayesian methods for the evaluation of the probabilities of the separate classes. The application is devoted to the analysis of the sleep stages of 184 individuals with different diagnoses, using EEG and further PSG signals. Data analysis points to an average increase of the length of the Wake stage by 2.7% per 10 years and a decrease of the length of the Rapid Eye Movement (REM) stages by 0.8% per 10 years. The mean classification accuracy for given sets of records and single EEG and multimodal features is 88.7% ( standard deviation, STD: 2.1) and 89.6% (STD:1.9), respectively. The proposed methods enable the use of adaptive learning processes for the detection and classification of health disorders based on prior specialist experience and man–machine interaction.

Detection of adulteration of small ruminant milk is very important for health and commercial reasons. New analytical and cost-effective methods need to be developed to detect new adulteration practices. In this work, we aimed to explore the ability of the MALDI-TOF mass spectrometry to detect bovine milk in caprine and ovine milk using samples from 18 dairy farms. Different levels of adulteration (0.5, 1, 5, 10, 20, 40, 60, and 80%) were analyzed during the lactation period of goat and sheep (in May, from 60 to 90 d in milk, and in August, from 150 to 180 d in milk). Two different ranges of peptide-protein spectra (500-4,000 Da; 4-20 kDa) were used to establish a calibration model for predicting the concentration of adulterant using partial least squares and generalized linear model with lasso regularization. The low molecular weight part of the spectra together with the generalized linear model with lasso regularization regression model appeared to have greater potential for our aim of detection of adulteration of small ruminants' milk. The subsequent prediction model was able to predict the concentration of bovine milk in caprine milk with a root mean square error of 11.4 and 17.0% in ovine milk. The results offer compelling evidence that MALDI-TOF can detect the adulteration of small ruminants' milk. However, the method is severely limited by (1) the complexity of the milk proteome resulting from the adulteration technique, (2) the potential degradation of thermolabile proteins, and (3) the genetic variability of tested samples. Additionally, the root mean square error of prediction based only on one individual sample adulteration series can drop down to 6.34% for quantification of adulterated caprine milk and 6.28% for adulterated ovine milk for the full set of concentrations or down to 2.33 and 4.00%, respectively, if we restrict only to low concentrations of adulteration (0, 0.5, 1, 5, 10%).