Organic waste utilization stands as a pivotal approach to ecological and economic sustainability. This study aimed to assess the stability, maturity, and evolution of co-composts comprising various blends of green waste (GW) and sheep manure (SM). Employing a diverse array of physico-chemical and biological parameters, we investigated the co-composting process over 120 days. Three types of garden waste (mixture of green waste (MGW), fallen leaves (FL), and grass cutting (GC)) were utilized. The results revealed significant compost transformation, evident by odor and insect absence and a shift to dark brown coloration, indicating maturation. The compost C2, derived from FL, exhibited superior soil amendment potential. Significantly, it exhibited a pH level of 6.80, an EC of 2.45 mS/cm, and an OM content of 55%, along with a C/N ratio of 16.15. Analysis of the macronutrients revealed values of 1.98% for TN, 3.22% for TP, and 0.61% for K. Crucially, the compost showed no phytotoxic effects and boasted a high GI of 94.20% and a low respiration rate of 4.02 mg/50 g, indicating its stability and appropriateness for agricultural application. Our findings underscore compost’s potential as an eco-friendly soil amendment, offering valuable insights for sustainable agricultural management and supporting the circular economy.

Two bulbous plant species, Squilla maura and Oncostema peruviana (Asparagaceae), show particularly interesting ornamental and medicinal characteristics. Micropropagation could be a promising alternative method to accelerate their naturally slow spreading pattern. This study focused on the effects of different growth regulators and culture media on callus induction and shoot regeneration, from which an effective protocol was established. Leaf explants were cultured on Murashige and Skoog (MS), MS/2, or B5 medium, containing one auxin—1-Naphthaleneacetic acid (NAA), 2,4-Dichlorophenoxyacetic acid (2.4-D), or 3-indolebutyric acid (IBA)—combined with the cytokinin 6-Benzylaminopurine (BAP), at various concentrations. All treatments resulted in callogenesis rates between 65–100% for both species. These treatments led to direct bulbil regeneration. Among the treatments, the B5 medium with 1 mgL⁻¹ BAP and 1 mgL⁻¹ 2,4-D gave the highest regeneration rate (89.2%) for O. peruviana, while the ½MS medium with 0.5 mgL⁻¹ BAP and 0.5 mgL⁻¹ NAA showed the highest regeneration rate (85.5%) for S. maura. The highest mean number of bulils per explant was 7.44 for O. peruviana on the MS medium (0.5 mgL⁻¹ BAP and 2 mgL⁻¹ IBA), and 9.5 for S. maura on the MS medium (1 mgL⁻¹ BAP and 0.5 mgL⁻¹ NAA). The regenerated bulbils were transferred for multiplication to the MS medium with a hormone combination (2 mgL⁻¹ BAP and 0.2 mgL⁻¹ NAA) which increased the multiplication rate compared to the callus induction me dium, with a highest recorded multiplication rate of 177 (O. peruviana) and 104.33 (S. maura). The propagation stage achieved the highest number of bulbils/explant after a second subculture for the two species. An efficient micropropagation protocol was established for S. maura, which answers our main objective, and it would contribute to their conservation and sustainable use in ornamental and medicinal applications.