Coconut (Cocos nucifera L.) is an important perennial crop adapted to a wide range of habitats. Although global coconut demand has increased sharply over the past few years, its production has been decreasing due to palm senility, as well as abiotic and biotic stresses. In fact, replanting efforts are impeded due to the lack of good quality seedlings. In vitro technologies have a great potential; however, their applications may take time to reach a commercial level. Therefore, traditional seed propagation is still critical to help meet the rising demand and its practice needs to be improved. To achieve an improved propagation via seeds, it is important to understand coconut fruit biology and its related issues. This review aims to provide a comprehensive summary of the existing knowledge on coconut fruit morpho-anatomy, germination biology, seed dispersal, distribution, fruit longevity and storage. This will help to identify gaps where future research efforts should be directed to improve traditional seed propagation.

The development of a cell suspension culture system for the scaling up of coconut embryogenic callus (EC) production would drastically improve efforts to achieve the large-scale production of high-quality clonal plantlets. To date, the hard nature of coconut EC appeared to be the main constraint for developing cell suspension cultures. Hence, this study attempted to acquire friable EC through the following approaches: The manipulation of (1) medium type and subculture frequency, (2) a reduced 2,4-dichlorophenoxy acetic acid concentration during subculture, (3) the nitrate level and the ammonium-to-nitrate ratio, and the addition of amino acid mixture, (4) the addition of L-proline, and (5) the reduction of medium nutrients. Unfortunately, none of these culture conditions produced friable coconut EC. Even though friable EC was not achieved via these approaches, some of the conditions were found to influence the formation of compact EC, therefore these results are important for further studies focused on somatic embryogenesis in coconut and other species.