The occurrence of Leptospira in mammals is an indicator for assessing potential health risks, particularly in tropical regions. Understanding their hosts’ habitat characteristics and spatial occurrence is essential to surveil them. This study aimed to determine the characteristics of the peridomiciles associated with the spatial occurrence of Leptospira hosts. We inventoried the characteristics of the peridomiciles. Blood serum was gathered from dogs, opossums, and rodents for a microagglutination essay to detect exposure to several serogroups of Leptospira. A generalized linear model with binomial distribution helped estimate risk ratios and 95% confidence intervals between a seropositive animal’s occurrence and the peridomiciles’ characteristics. For estimates with the occurrence of one or more seropositive animals, a multinomial model was fitted. The seroprevalence in rodents was 81.8%; in opossums 31.2%; and 56.5% for dogs. The most frequent serogroup in dogs and rodents was Canicola. In opossums, the seroreaction was against Tarassovi, Ballum, Sejroe, and Cynopteri. The results showed that the peridomicile area (m²), the geographic polygon, and the accumulation of plastic containers (PET) were characteristics related to the occurrence of seropositive animals. The results revealed that the peridomicile’s characteristics and spatial distribution in the locality help explain the occurrence of Leptospira hosts.

La leptospirosis es la enfermedad zoonótica más ampliamente distribuida a nivel mundial, la cual es causada por 17 especies de espiroquetas del género Leptospira. Los miembros patógenos de este género bacteriano se relacionan con una amplia gama de hospederos vertebrados, en particular con cánidos, en los cuales pueden generar un amplio abanico de signos clínicos. Dado que se ha identificado que los perros fungen como una unidad centinela que permite identificar el grado de diseminación de estas bacterias en el medio, la vigilancia epidemiológica resulta prioritaria en estos hospederos. Por ello, el objetivo del presente trabajo fue identificar la seropositividad de la leptospirosis en cánidos de libre rango y ferales en una reserva ecológica urbana en la Ciudad de México, y explorar sus implicaciones en salud pública y medicina de la conservación. Se obtuvieron muestras de suero de 19 caninos, a los cuales se realizó la prueba de aglutinación microscópica (MAT, por sus siglas en inglés). El porcentaje de anticuerpos anti–Leptospira spp. en perros ferales fue de 100 % (IC 95 % 82,3–100), con una seroreactividad para tres serovariedades. Este es el primer trabajo realizado que identifica el grado de exposición a Leptospira spp. en perros ferales en México, en particular en una reserva ecológica.

Cattle raising is the most important livestock activity in Mexico, highlighting the fact that the Country is eight place worldwide in the production of bovine meat. However, cattle can be affected by leptospirosis (a bacterial disease caused by 17 species of spirochetes of the genus Leptospira), which cause reproductive problems that translate into great economic losses. Additionally, these zoonotic agents can cause a rapidly evolving febrile condition in humans, which can be solved after the first week of symptoms or could progress to develop a severe late-phase manifestations. Despite the great impact of these agents on the economy and Public Health, epidemiological surveillance against the infectious disease that cause is not present in municipal slaughterhouses (MS). Therefore, the aim of this study was to identify, using serological and molecular methods, the circulating Leptospira species in three MS in Veracruz State. The frequency of anti-Leptospira antibodies was 67.5%. Additionally, five sequences were recovered that were 99% similar to L. interrogans. This work represents the first national effort for the evaluation of MS as sentinel units, that allow establishing the diversity of species of the genus Leptospira that circulate in cattle and establishing intervention measures for workers risk mitigation, who come into contact with the fluids and organs of infected animals.

The occurrence of Leptospira in mammals is an indicator for assessing potential health risks, particularly in tropical regions. Understanding their hosts' habitat characteristics and spatial occurrence is essential to surveil them. This study aimed to determine the characteristics of the peridomiciles associated with the spatial occurrence of Leptospira hosts. We inventoried the characteristics of the peridomiciles. Blood serum was gathered from dogs, opossums, and rodents for a microagglutination essay to detect exposure to several serogroups of Leptospira. A generalized linear model with binomial distribution helped estimate risk ratios and 95% confidence intervals between a seropositive animal's occurrence and the peridomiciles' characteristics. For estimates with the occurrence of one or more seropositive animals, a multinomial model was fitted. The seroprevalence in rodents was 81.8%; in opossums 31.2%; and 56.5% for dogs. The most frequent serogroup in dogs and rodents was Canicola. In opossums, the seroreaction was against Tarassovi, Ballum, Sejroe, and Cynopteri. Results showed that the peridomicile area (m2), the geographic polygon, and the accumulation of plastic containers (PET) were characteristics related to the occurrence of seropositive animals. The results revealed that the peridomicile's characteristics and spatial distribution in the locality help explain the occurrence of Leptospira hosts.