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Mycotoxins are toxic metabolites of filamentous fungi. Previous studies demonstrated the co-occurrence of Fusarium and Alternaria toxins, including zearalenone (ZEN), ZEN metabolites, and alternariol (AOH). These xenoestrogenic mycotoxins appear in soy-based meals and dietary supplements, resulting in the co-exposure to ZEN and AOH with the phytoestrogen genistein (GEN). In this study, the cytotoxic and estrogenic effects of ZEN, reduced ZEN metabolites, AOH, and GEN are examined to evaluate their individual and combined impacts. Our results demonstrate that reduced ZEN metabolites, AOH, and GEN can aggravate ZEN-induced toxicity; in addition, the compounds tested exerted mostly synergism or additive combined effects regarding cytotoxicity and/or estrogenicity. Therefore, these observations underline the importance and the considerable risk of mycotoxin co-exposure and the combined effects of mycoestrogens with phytoestrogens.
Adrienn Balázs; Zelma Faisal; Rita Csepregi; Tamás Kőszegi; Balázs Kriszt; István Szabó; Miklós Poór. In Vitro Evaluation of the Individual and Combined Cytotoxic and Estrogenic Effects of Zearalenone, Its Reduced Metabolites, Alternariol, and Genistein. International Journal of Molecular Sciences 2021, 22, 6281 .
AMA StyleAdrienn Balázs, Zelma Faisal, Rita Csepregi, Tamás Kőszegi, Balázs Kriszt, István Szabó, Miklós Poór. In Vitro Evaluation of the Individual and Combined Cytotoxic and Estrogenic Effects of Zearalenone, Its Reduced Metabolites, Alternariol, and Genistein. International Journal of Molecular Sciences. 2021; 22 (12):6281.
Chicago/Turabian StyleAdrienn Balázs; Zelma Faisal; Rita Csepregi; Tamás Kőszegi; Balázs Kriszt; István Szabó; Miklós Poór. 2021. "In Vitro Evaluation of the Individual and Combined Cytotoxic and Estrogenic Effects of Zearalenone, Its Reduced Metabolites, Alternariol, and Genistein." International Journal of Molecular Sciences 22, no. 12: 6281.
Melissopalynology, antioxidant capacity and mineral and toxic element contents were analyzed in eight types of Hungarian honeys. Based on color, two groups were distinguished: light honeys comprised acacia, amorpha, phacelia and linden honeys; while dark honeys included sunflower, chestnut, fennel and sage honeys, with 100 to 300 and 700 to 1500 mAU, respectively. The unifloral origin of each sample was supported using pollen analysis. The absorbance of honey correlated positively with antioxidant capacity determined by three different methods (TRC, DPPH, ORAC), and also with mineral content. The exception was the light amber linden honey with significantly higher K content and antiradical activity than other light honeys. The Mn, Zn and Fe contents were the highest in chestnut, sunflower and fennel honeys, respectively. The black meadow sage honey performed best regarding the content of other elements and antioxidant activity. The concentrations of several toxic elements were below the detection limit in the samples, indicating their good quality. The principal component analysis (PCA) revealed correlations between different antioxidant assays and minerals, and furthermore, confirmed the botanical authentication of the honeys based on the studied parameters. To our best knowledge, the present study is the first to provide a complex analysis of quality parameters of eight unifloral Hungarian honeys.
Alexandra Bodó; Lilla Radványi; Tamás Kőszegi; Rita Csepregi; Dávid Nagy; Ágnes Farkas; Marianna Kocsis. Quality Evaluation of Light- and Dark-Colored Hungarian Honeys, Focusing on Botanical Origin, Antioxidant Capacity and Mineral Content. Molecules 2021, 26, 2825 .
AMA StyleAlexandra Bodó, Lilla Radványi, Tamás Kőszegi, Rita Csepregi, Dávid Nagy, Ágnes Farkas, Marianna Kocsis. Quality Evaluation of Light- and Dark-Colored Hungarian Honeys, Focusing on Botanical Origin, Antioxidant Capacity and Mineral Content. Molecules. 2021; 26 (9):2825.
Chicago/Turabian StyleAlexandra Bodó; Lilla Radványi; Tamás Kőszegi; Rita Csepregi; Dávid Nagy; Ágnes Farkas; Marianna Kocsis. 2021. "Quality Evaluation of Light- and Dark-Colored Hungarian Honeys, Focusing on Botanical Origin, Antioxidant Capacity and Mineral Content." Molecules 26, no. 9: 2825.
Polyphenolic compounds (including flavonoids, chalcones, phenolic acids, and furanocoumarins) represent a common part of our diet, but are also the active ingredients of several dietary supplements and/or medications. These compounds undergo extensive metabolism by human biotransformation enzymes and the microbial flora of the colon. CYP2D6 enzyme metabolizes approximately 25% of the drugs, some of which has narrow therapeutic window. Therefore, its inhibition can lead to the development of pharmacokinetic interactions and the disruption of drug therapy. In this study, the inhibitory effects of 17 plant-derived compounds and 19 colonic flavonoid metabolites on CYP2D6 were examined, employing two assays with different test substrates. The O-demethylation of dextromethorphan was tested employing CypExpress 2D6 kit coupled to HPLC analysis; while the O-demethylation of another CYP2D6 specific substrate (AMMC) was investigated in a plate reader assay with BioVision Fluorometric CYP2D6 kit. Interestingly, some compounds (e.g., bergamottin) inhibited both dextromethorphan and AMMC demethylation; however, certain substances proved to be inhibitors only in one of the assays applied. Our results demonstrate that some polyphenols and colonic metabolites are inhibitors of CYP2D6-catalyzed reactions. Nevertheless, the inhibitory effects showed strong substrate dependence.
Eszter Fliszár-Nyúl; Violetta Mohos; Rita Csepregi; Přemysl Mladěnka; Miklós Poór. Inhibitory effects of polyphenols and their colonic metabolites on CYP2D6 enzyme using two different substrates. Biomedicine & Pharmacotherapy 2020, 131, 110732 .
AMA StyleEszter Fliszár-Nyúl, Violetta Mohos, Rita Csepregi, Přemysl Mladěnka, Miklós Poór. Inhibitory effects of polyphenols and their colonic metabolites on CYP2D6 enzyme using two different substrates. Biomedicine & Pharmacotherapy. 2020; 131 ():110732.
Chicago/Turabian StyleEszter Fliszár-Nyúl; Violetta Mohos; Rita Csepregi; Přemysl Mladěnka; Miklós Poór. 2020. "Inhibitory effects of polyphenols and their colonic metabolites on CYP2D6 enzyme using two different substrates." Biomedicine & Pharmacotherapy 131, no. : 110732.
Ochratoxins, patulin, deoxynivalenol, and T-2 toxin are mycotoxins, and common contaminants in food and drinks. Human serum albumin (HSA) forms complexes with certain mycotoxins. Since HSA can affect the toxicokinetics of bound ligand molecules, the potential interactions of ochratoxin B (OTB), ochratoxin C (OTC), patulin, deoxynivalenol, and T-2 toxin with HSA were examined, employing spectroscopic (fluorescence, UV, and circular dichroism) and ultrafiltration techniques. Furthermore, the influence of albumin on the cytotoxicity of these xenobiotics was also evaluated in cell experiments. Fluorescence studies showed the formation of highly stable OTB–HSA and OTC–HSA complexes. Furthermore, fluorescence quenching and circular dichroism measurements suggest weak or no interaction of patulin, deoxynivalenol, and T-2 toxin with HSA. In ultrafiltration studies, OTB and OTC strongly displaced the Sudlow’s site I ligand warfarin, while other mycotoxins tested did not affect either the albumin binding of warfarin or naproxen. The presence of HSA significantly decreased or even abolished the OTB- and OTC-induced cytotoxicity in cell experiments; however, the toxic impacts of patulin, deoxynivalenol, and T-2 toxin were not affected by HSA. In summary, the complex formation of OTB and OTC with albumin is relevant, whereas the interactions of patulin, deoxynivalenol, and T-2 toxin with HSA may have low toxicological importance.
Zelma Faisal; Virág Vörös; Eszter Fliszár-Nyúl; Beáta Lemli; Sándor Kunsági-Máté; Rita Csepregi; Tamás Kőszegi; Ferenc Zsila; Miklós Poór. Probing the Interactions of Ochratoxin B, Ochratoxin C, Patulin, Deoxynivalenol, and T-2 Toxin with Human Serum Albumin. Toxins 2020, 12, 1 .
AMA StyleZelma Faisal, Virág Vörös, Eszter Fliszár-Nyúl, Beáta Lemli, Sándor Kunsági-Máté, Rita Csepregi, Tamás Kőszegi, Ferenc Zsila, Miklós Poór. Probing the Interactions of Ochratoxin B, Ochratoxin C, Patulin, Deoxynivalenol, and T-2 Toxin with Human Serum Albumin. Toxins. 2020; 12 (6):1.
Chicago/Turabian StyleZelma Faisal; Virág Vörös; Eszter Fliszár-Nyúl; Beáta Lemli; Sándor Kunsági-Máté; Rita Csepregi; Tamás Kőszegi; Ferenc Zsila; Miklós Poór. 2020. "Probing the Interactions of Ochratoxin B, Ochratoxin C, Patulin, Deoxynivalenol, and T-2 Toxin with Human Serum Albumin." Toxins 12, no. 6: 1.
Medicinal plants are widely used in folk medicine but quite often their composition and biological effects are hardly known. Our study aimed to analyze the composition, cytotoxicity, antimicrobial, antioxidant activity and cellular migration effects of Anthyllis vulneraria, Fuchsia magellanica, Fuchsia triphylla and Lysimachia nummularia used in the Romanian ethnomedicine for wounds. Liquid chromatography with mass spectrometry (LC-MS/MS) was used to analyze 50% (v/v) ethanolic and aqueous extracts of the plants’ leaves. Antimicrobial activities were estimated with a standard microdilution method. The antioxidant properties were evaluated by validated chemical cell-free and biological cell-based assays. Cytotoxic effects were performed on mouse fibroblasts and human keratinocytes with a plate reader-based method assessing intracellular adenosine triphosphate (ATP), nucleic acid and protein contents and also by a flow cytometer-based assay detecting apoptotic–necrotic cell populations. Cell migration to cover cell-free areas was visualized by time-lapse phase-contrast microscopy using standard culture inserts. Fuchsia species showed the strongest cytotoxicity and the highest antioxidant and antimicrobial activity. However, their ethanolic extracts facilitated cell migration, most probably due to their various phenolic acid, flavonoid and anthocyanin derivatives. Our data might serve as a basis for further animal experiments to explore the complex action of Fuchsia species in wound healing assays.
Rita Csepregi; Viktória Temesfői; Sourav Das; Ágnes Alberti; Csenge Anna Tóth; Róbert Herczeg; Nóra Papp; Tamás Kőszegi. Cytotoxic, Antimicrobial, Antioxidant Properties and Effects on Cell Migration of Phenolic Compounds of Selected Transylvanian Medicinal Plants. Antioxidants 2020, 9, 166 .
AMA StyleRita Csepregi, Viktória Temesfői, Sourav Das, Ágnes Alberti, Csenge Anna Tóth, Róbert Herczeg, Nóra Papp, Tamás Kőszegi. Cytotoxic, Antimicrobial, Antioxidant Properties and Effects on Cell Migration of Phenolic Compounds of Selected Transylvanian Medicinal Plants. Antioxidants. 2020; 9 (2):166.
Chicago/Turabian StyleRita Csepregi; Viktória Temesfői; Sourav Das; Ágnes Alberti; Csenge Anna Tóth; Róbert Herczeg; Nóra Papp; Tamás Kőszegi. 2020. "Cytotoxic, Antimicrobial, Antioxidant Properties and Effects on Cell Migration of Phenolic Compounds of Selected Transylvanian Medicinal Plants." Antioxidants 9, no. 2: 166.
Zearalenone is a xenoestrogenic mycotoxin produced by Fusarium species. High exposure with zearalenone induces reproductive disorders worldwide. Cyclodextrins are ring-shaped host molecules built up from glucose units. The apolar cavity of cyclodextrins can entrap so-called guest molecules. The formation of highly stable host-guest type complexes with cyclodextrins can decrease the biological effect of the guest molecule. Therefore, cyclodextrins may be suitable to decrease the toxicity of some xenobiotics even after the exposure. In this study, the protective effect of beta-cyclodextrins against zearalenone-induced toxicity was investigated in HeLa cells and zebrafish embryos. Fluorescence spectroscopic studies demonstrated the formation of stable complexes of zearalenone with sulfobutyl-, methyl-, and succinyl-methyl-substituted beta-cyclodextrins at pH 7.4 (K = 1.4–4.7 × 104 L/mol). These chemically modified cyclodextrins considerably decreased or even abolished the zearalenone-induced loss of cell viability in HeLa cells and mortality in zebrafish embryos. Furthermore, the sublethal effects of zearalenone were also significantly alleviated by the co-treatment with beta-cyclodextrins. To test the estrogenic effect of the mycotoxin, a transgenic bioindicator zebrafish model (Tg(vtg1:mCherry)) was also applied. Our results suggest that the zearalenone-induced vitellogenin production is partly suppressed by the hepatotoxicity of zearalenone in zebrafish. This study demonstrates that the formation of stable zearalenone-cyclodextrin complexes can strongly decrease or even abolish the zearalenone-induced toxicity, both in vitro and in vivo. Therefore, cyclodextrins appear as promising new mycotoxin binders.
Zelma Faisal; Edina Garai; Rita Csepregi; Katalin Bakos; Eszter Fliszár-Nyúl; Lajos Szente; Adrienn Balázs; Mátyás Cserháti; Tamás Kőszegi; Béla Urbányi; Zsolt Csenki; Miklós Poór. Protective effects of beta-cyclodextrins vs. zearalenone-induced toxicity in HeLa cells and Tg(vtg1:mCherry) zebrafish embryos. Chemosphere 2019, 240, 124948 .
AMA StyleZelma Faisal, Edina Garai, Rita Csepregi, Katalin Bakos, Eszter Fliszár-Nyúl, Lajos Szente, Adrienn Balázs, Mátyás Cserháti, Tamás Kőszegi, Béla Urbányi, Zsolt Csenki, Miklós Poór. Protective effects of beta-cyclodextrins vs. zearalenone-induced toxicity in HeLa cells and Tg(vtg1:mCherry) zebrafish embryos. Chemosphere. 2019; 240 ():124948.
Chicago/Turabian StyleZelma Faisal; Edina Garai; Rita Csepregi; Katalin Bakos; Eszter Fliszár-Nyúl; Lajos Szente; Adrienn Balázs; Mátyás Cserháti; Tamás Kőszegi; Béla Urbányi; Zsolt Csenki; Miklós Poór. 2019. "Protective effects of beta-cyclodextrins vs. zearalenone-induced toxicity in HeLa cells and Tg(vtg1:mCherry) zebrafish embryos." Chemosphere 240, no. : 124948.
A fluorescence-based enzymatic microplate intracellular glucose assay was designed and fully validated. The method was tested in a hepatocellular cancer cell line (HepG2). Our novel one-step extraction reagent gave stable cell lysates for glucose, adenosine triphosphate (ATP), and total protein determination from the same sample. Limit of detection for glucose was 0.13 µM (26 pmol/well), which is superior to commercially available glucose assays. Both intra- and interday assay imprecision in HepG2 cultures were less than 12% coefficient of variance (CV). In cell lysates spiked with glucose, recovery at two levels varied between 83.70% and 91.81%, and both linearity and stability were acceptable. HepG2 cells treated with agents affecting glucose uptake/metabolism (phloretin, quercetin, quercetin-3′-sulfate, NaF, 3-bromopyruvate, NaN3, oligomycin A, ochratoxin A, cytochalasin B, and anti-GLUT1 antibody) showed dose-dependent changes in glucose and ATP levels without total protein (cell) loss. Finally, we performed flow cytometric glucose uptake measurement in the treated cells using 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxyglucose fluorescent glucose analog. Glucose uptake did not always mirror the intracellular glucose levels, which most likely reflects the differences between the two methodologies. However, interpreting data obtained by both methods and taking ATP/protein levels at the same time, one can get information on the mode of action of the compounds.
Rita Csepregi; Viktória Temesfői; Nikolett Sali; Miklós Poór; Paul W. Needs; Paul A. Kroon; Tamás Kőszegi. A One-Step Extraction and Luminescence Assay for Quantifying Glucose and ATP Levels in Cultured HepG2 Cells. International Journal of Molecular Sciences 2018, 19, 2670 .
AMA StyleRita Csepregi, Viktória Temesfői, Nikolett Sali, Miklós Poór, Paul W. Needs, Paul A. Kroon, Tamás Kőszegi. A One-Step Extraction and Luminescence Assay for Quantifying Glucose and ATP Levels in Cultured HepG2 Cells. International Journal of Molecular Sciences. 2018; 19 (9):2670.
Chicago/Turabian StyleRita Csepregi; Viktória Temesfői; Nikolett Sali; Miklós Poór; Paul W. Needs; Paul A. Kroon; Tamás Kőszegi. 2018. "A One-Step Extraction and Luminescence Assay for Quantifying Glucose and ATP Levels in Cultured HepG2 Cells." International Journal of Molecular Sciences 19, no. 9: 2670.
Ochratoxin A (OTA) is a nephrotoxic mycotoxin. Roasting of OTA-contaminated coffee results in the formation of 2′R-ochratoxin A (2′R-OTA), which appears in the blood of coffee drinkers. Human serum albumin (HSA) binds 2′R-OTA (and OTA) with high affinity; therefore, albumin may influence the tissue uptake and elimination of ochratoxins. We aimed to investigate the binding site of 2′R-OTA (verses OTA) in HSA and the displacing effects of site markers to explore which molecules can interfere with its albumin-binding. Affinity of 2′R-OTA toward albumins from various species (human, bovine, porcine and rat) was tested to evaluate the interspecies differences regarding 2′R-OTA-albumin interaction. Thermodynamic studies were performed to give a deeper insight into the molecular background of the complex formation. Besides fluorescence spectroscopic and modeling studies, effects of HSA, and fetal bovine serum on the cytotoxicity of 2′R-OTA and OTA were tested in MDCK kidney cell line in order to demonstrate the influence of albumin-binding on the cellular uptake of ochratoxins. Site markers displaced more effectively 2′R-OTA than OTA from HSA. Fluorescence and binding constants of 2′R-OTA-albumin and OTA-albumin complexes showed different tendencies. Albumin significantly decreased the cytotoxicity of ochratoxins. 2′R-OTA, even at sub-toxic concentrations, increased the toxic action of OTA.
Zelma Faisal; Diána Derdák; Beáta Lemli; Sándor Kunsági-Máté; Mónika Bálint; Csaba Hetényi; Rita Csepregi; Tamás Kőszegi; Franziska Sueck; Benedikt Cramer; Hans-Ulrich Humpf; Miklós Poór. Interaction of 2′R-ochratoxin A with Serum Albumins: Binding Site, Effects of Site Markers, Thermodynamics, Species Differences of Albumin-binding, and Influence of Albumin on Its Toxicity in MDCK Cells. Toxins 2018, 10, 353 .
AMA StyleZelma Faisal, Diána Derdák, Beáta Lemli, Sándor Kunsági-Máté, Mónika Bálint, Csaba Hetényi, Rita Csepregi, Tamás Kőszegi, Franziska Sueck, Benedikt Cramer, Hans-Ulrich Humpf, Miklós Poór. Interaction of 2′R-ochratoxin A with Serum Albumins: Binding Site, Effects of Site Markers, Thermodynamics, Species Differences of Albumin-binding, and Influence of Albumin on Its Toxicity in MDCK Cells. Toxins. 2018; 10 (9):353.
Chicago/Turabian StyleZelma Faisal; Diána Derdák; Beáta Lemli; Sándor Kunsági-Máté; Mónika Bálint; Csaba Hetényi; Rita Csepregi; Tamás Kőszegi; Franziska Sueck; Benedikt Cramer; Hans-Ulrich Humpf; Miklós Poór. 2018. "Interaction of 2′R-ochratoxin A with Serum Albumins: Binding Site, Effects of Site Markers, Thermodynamics, Species Differences of Albumin-binding, and Influence of Albumin on Its Toxicity in MDCK Cells." Toxins 10, no. 9: 353.
Green fluorescent protein (GFP) is considered to be suitable for cell viability testing. In our study, GFP transfected A549 lung carcinoma cell line was treated with sodium fluoride (NaF), cycloheximide (CHX) and ochratoxin A (OTA). GFP fluorescence, intracellular ATP, nucleic acid and protein contents were quantified by a luminescence microplate assay developed in our laboratory. Flow cytometry was used to confirm the findings and to assess the intensity of GFP during different types of cell death. A 24 h NaF and CHX exposure caused a dramatic decrease in ATP contents (p < 0.05) compared with those of the controls. GFP fluorescence of the cells was in close correlation with total protein; however, GFP/ATP increased at NaF and decreased at CHX treatments (p < 0.05). ATP/protein and ATP/propidium iodide (PI) were largely decreased at NaF exposure in a dose-dependent manner (p < 0.05), while CHX and OTA showed markedly fewer effects. Both treatments caused apoptosis/necrosis at different rates. NaF induced mainly late apoptosis while OTA, mainly apoptosis. CHX effects varied by the incubation time with 100-fold elevation in late apoptotic cells at 24 h treatment. GFP intensity did not show a significant difference between live and apoptotic populations. Our results suggest when using GFP, a multiparametric assay is necessary for more precise interpretation of cell viability.
Rita Csepregi; Viktória Temesfői; Miklós Poór; Zsuzsanna Faust; Tamás Kõszegi. Green Fluorescent Protein-Based Viability Assay in a Multiparametric Configuration. Molecules 2018, 23, 1575 .
AMA StyleRita Csepregi, Viktória Temesfői, Miklós Poór, Zsuzsanna Faust, Tamás Kõszegi. Green Fluorescent Protein-Based Viability Assay in a Multiparametric Configuration. Molecules. 2018; 23 (7):1575.
Chicago/Turabian StyleRita Csepregi; Viktória Temesfői; Miklós Poór; Zsuzsanna Faust; Tamás Kõszegi. 2018. "Green Fluorescent Protein-Based Viability Assay in a Multiparametric Configuration." Molecules 23, no. 7: 1575.
Resazurin (or Alamar Blue) is a poorly fluorescent dye. During the cellular reduction of resazurin, its highly fluorescent product resorufin is formed. Resazurin assay is a commonly applied method to investigate viability of bacterial and mammalian cells. In this study, the interaction of resazurin and resorufin with β-cyclodextrins was investigated employing spectroscopic and molecular modeling studies. Furthermore, the influence of β-cyclodextrins on resazurin-based cell viability assay was also tested. Both resazurin and resorufin form stable complexes with the examined β-cyclodextrins (2.0–3.1 × 103 and 1.3–1.8 × 103 L/mol were determined as binding constants, respectively). Cells were incubated for 30 and 120 min and treated with resazurin and/or β-cyclodextrins. Our results suggest that cyclodextrins are able to interfere with the resazurin-based cell viability assay that presumably results from the following mechanisms: (1) inhibition of the cellular uptake of resazurin and (2) enhancement of the fluorescence signal of the formed resorufin.
Rita Csepregi; Beáta Lemli; Sándor Kunsági-Máté; Lajos Szente; Tamás Kõszegi; Balázs Németi; Miklós Poór. Complex Formation of Resorufin and Resazurin with Β-Cyclodextrins: Can Cyclodextrins Interfere with a Resazurin Cell Viability Assay? Molecules 2018, 23, 382 .
AMA StyleRita Csepregi, Beáta Lemli, Sándor Kunsági-Máté, Lajos Szente, Tamás Kõszegi, Balázs Németi, Miklós Poór. Complex Formation of Resorufin and Resazurin with Β-Cyclodextrins: Can Cyclodextrins Interfere with a Resazurin Cell Viability Assay? Molecules. 2018; 23 (2):382.
Chicago/Turabian StyleRita Csepregi; Beáta Lemli; Sándor Kunsági-Máté; Lajos Szente; Tamás Kõszegi; Balázs Németi; Miklós Poór. 2018. "Complex Formation of Resorufin and Resazurin with Β-Cyclodextrins: Can Cyclodextrins Interfere with a Resazurin Cell Viability Assay?" Molecules 23, no. 2: 382.
Nikolett Sali; Rita Csepregi; Tamás Kőszegi; Sándor Kunsági-Máté; Lajos Szente; Miklós Poór. Complex formation of flavonoids fisetin and geraldol with β-cyclodextrins. Journal of Luminescence 2018, 194, 82 -90.
AMA StyleNikolett Sali, Rita Csepregi, Tamás Kőszegi, Sándor Kunsági-Máté, Lajos Szente, Miklós Poór. Complex formation of flavonoids fisetin and geraldol with β-cyclodextrins. Journal of Luminescence. 2018; 194 ():82-90.
Chicago/Turabian StyleNikolett Sali; Rita Csepregi; Tamás Kőszegi; Sándor Kunsági-Máté; Lajos Szente; Miklós Poór. 2018. "Complex formation of flavonoids fisetin and geraldol with β-cyclodextrins." Journal of Luminescence 194, no. : 82-90.
To investigate the antioxidant activity, total phenolic and total tannin content of the pericarp and the seed of Coffea benghalensis (C. benghalensis) and Coffea liberica compared to Coffea arabica (C. arabica).The antioxidant potential, total tannin and polyphenol contents of the immature and mature seed and pericarp of C. benghalensis and Coffea liberica were quantified and compared to C. arabica. Enhanced chemiluminescence (ECL), 2,2-diphenyl-1-picrylhydrazyl (DPPH), oxygen radical absorbance capacity, Folin-Ciocalteau method and total tannin content assays were used.Trolox equivalent (TE/g plant material) values obtained by ECL and DPPH methods showed loose correlation (r(2) = 0.587) while those measured by oxygen radical absorbance capacity assay were higher without correlation in each plant. A closer correlation was detected between the ECL method and the percentage antioxidant activity of the DPPH technique (r(2) = 0.610 7) in each species, however the immature pericarp of C. benghalensis showed much higher DPPH scavenging potential than was seen in the ECL assay. The immature pericarp of C. benghalensis expressed the highest tannin and polyphenol content, and a high polyphenol level was also detected in the immature seed of C. arabica. The immature pericarp of Bengal and Liberian coffees showed the largest amount of phenolic contents.The obtained data highlight the potential role of C. benghalensis as a new source of natural antioxidants and polyphenols compared to C. arabica.
Éva Brigitta Patay; Nikolett Sali; Tamás Kőszegi; Rita Csepregi; Viktória Lilla Balázs; Tibor Sebastian Németh; Nóra Papp. Antioxidant potential, tannin and polyphenol contents of seed and pericarp of three Coffea species. Asian Pacific Journal of Tropical Medicine 2016, 9, 366 -371.
AMA StyleÉva Brigitta Patay, Nikolett Sali, Tamás Kőszegi, Rita Csepregi, Viktória Lilla Balázs, Tibor Sebastian Németh, Nóra Papp. Antioxidant potential, tannin and polyphenol contents of seed and pericarp of three Coffea species. Asian Pacific Journal of Tropical Medicine. 2016; 9 (4):366-371.
Chicago/Turabian StyleÉva Brigitta Patay; Nikolett Sali; Tamás Kőszegi; Rita Csepregi; Viktória Lilla Balázs; Tibor Sebastian Németh; Nóra Papp. 2016. "Antioxidant potential, tannin and polyphenol contents of seed and pericarp of three Coffea species." Asian Pacific Journal of Tropical Medicine 9, no. 4: 366-371.