The fruit supply chain requires simple, non-destructive, and fast tools for quality evaluation both in the field and during the post-harvest phase. In this study, a portable visible and near-infrared (Vis/NIR) spectrophotometer and a portable Vis/NIR hyperspectral imaging (HSI) device were tested to highlight genetic differences among apricot cultivars, and to develop multi-cultivar and multi-year models for the most important marketable attributes (total soluble solids, TSS; titratable acidity, TA; dry matter, DM). To do this, the fruits of seventeen cultivars from a single experimental orchard harvested at the commercial maturity stage were considered. Spectral data emphasized genetic similarities and differences among the cultivars, capturing changes in the pigment content and macro components of the apricot samples. In recent years, machine learning techniques, such as artificial neural networks (ANNs), have been successfully applied to more efficiently extract valuable information from spectral data and to accurately predict quality traits. In this study, prediction models were developed based on a multilayer perceptron artificial neural network (ANN-MLP) combined with the Levenberg–Marquardt learning algorithm. Regarding the Vis/NIR spectrophotometer dataset, good predictive performances were achieved for TSS (R² = 0.855) and DM (R² = 0.857), while the performance for TA was unsatisfactory (R² = 0.681). In contrast, the optimal predictive ability was found for models of the HSI dataset (TSS: R² = 0.904; DM: R² = 0.918, TA: R² = 0.811), as confirmed by external validation. Moreover, the ANN allowed us to identify the most predictive input spectral regions for each model. The results showed the potential of Vis/NIR spectroscopy as an alternative to traditional destructive methods to monitor the qualitative traits of apricot fruits, reducing the time and costs of analyses.

Among the strategies proposed to reduce the formation of acrylamide in bakery products, the use of asparaginase is considered one of the most promising, also due to its limited impact on the sensory quality of the final product. Asparagine, the key precursor of acrylamide that is selectively hydrolysed by the asparaginase treatment, is generally not considered a major contributor to the overall flavour and colour of baked foods. This study investigates the effect of three addition levels of the enzyme asparaginase (500, 750, 1000 ASNU compared to a not added control) and three different preparation conditions (without resting time and with 15 min resting time at 20 °C or 50 °C) on asparagine content in the dough and on the formation of Volatile Organic Compounds (VOCs) and colour of shortbread biscuits. Results showed that the addition of asparaginase, at all levels, markedly reduced the asparagine content in the dough, with limited effects on the VOC profile and colour. The examined preparation conditions significantly affected the VOC profile: the application of a resting time at 20 °C tended to promote the formation of VOCs through lipid oxidation while reducing the level of many MR-related VOCs. This last effect seemed to parallel the slight reduction of the browning index observed when the resting time was applied. Results suggest that the use of asparaginase does not markedly affect the VOC profile of shortbread biscuits, thus confirming its limited effects on sensory-related quality attributes of baked foods.

The effects of asparaginase treatment and conditions of its use (enzyme dose and dough resting time) on the formation of heat-induced contaminants, namely, acrylamide (ACR) and 5-hydroxymethylfurfural (HMF), were investigated in wholemeal wheat biscuits. In addition, potential impacts on sensory-related quality attributes were assessed through instrumental analysis of colour, texture, and volatile organic compounds (VOCs). Three levels of asparaginase addition (100, 300, and 500 ASNU kg⁻¹ flour) were tested and compared with an untreated control, with and without the application of a 15 min dough resting step. Results confirmed the effectiveness of asparaginase in reducing ACR levels in the final biscuits, showing a dose-dependent response with reductions of up to 68% at 500 ASNU kg⁻¹ flour. A dough resting step tend to further lower ACR content. In contrast, asparaginase treatment did not exert a consistent effect on HMF formation, whereas dough resting tended to decrease it. Moreover, asparaginase addition at the highest dose reduced the level of several Maillard reaction-derived VOCs by approximately 30%. Overall, asparaginase treatment markedly reduced ACR formation while causing changes in some odour-active compounds in biscuits. Further sensory evaluation will be necessary to determine whether these changes are perceptible and may influence consumer acceptance.

Sulforaphane (SF), the renowned hydrolysis product of glucoraphanin from broccoli, is known to undergo thermal degradation to 3-butenyl isothiocyanate (3-BITC) during gas chromatography–mass spectrometry (GC-MS) analysis. In this study, the effects on SF degradation associated with exposure to oven temperature during column transit, passage through the split/splitless injector, and through the transfer line to the mass spectrometer were investigated. Massive degradation was observed during column transit when the oven temperature exceeded 150 °C. Degradation during the passage through the injector increased with temperature, from 150 °C to 250 °C, and with decreasing split ratio, from 100:1 to 20:1. Conventional splitless conditions with the injector temperature set at 250 °C resulted in a degradation > 60%. Operating the GC-MS system with oven temperature below 150 °C prior to SF elution, an injector at 225 °C and a split ratio of 20:1, SF degradation was reduced to less than 1%. Under these optimized analytical conditions, the concentration of free SF in two broccoli-based dietary supplements was estimated based on external calibration and using 2,6-dimethylphenyl isothiocyanate as an internal standard. The GC-MS method proved to be a valuable tool for the quality evaluation of SF-labeled supplements integrated with the official EU ISO 9167:2019 HPLC-PDA glucosinolates analysis.

The widespread practice of multiple harvests is known to significantly affect the quality of rocket leaves. Thus, ready-to-eat wild rocket (Diplotaxis tenuifolia L.) was produced under commercial conditions using leaves from five different varieties, harvested at both the first and the second cut, with a 21-day interval between cuts. Both the sensory and nutritional quality of rocket leaves were evaluated one day after packaging (beginning of shelf-life) and after an additional six days of storage at 6 °C (end of shelf-life). At the beginning of the shelf-life, first-cut leaves generally exhibited a more intense flavor, corresponding to a higher level of isothiocyanates, compared to second-cut leaves. At the end of shelf-life, first-cut leaves showed a greater susceptibility to off-flavor development, corresponding to the release of higher levels of sulfur compounds within the packages. First-cut leaves showed higher content of ascorbic acid and folate with respect of second-cut leaves, both at the beginning and the end of the shelf-life. The commonly held assumption that successive cuts yield leaves with more intense flavor compared to the first cut does not appear to be confirmed. It is likely that the differences in quality observed between leaves from the first and second cuts are not attributable solely to the cutting process or to the sequence of multiple harvest per se, but rather to significant variations in environmental conditions, particularly temperature and light, during the leaf growth period between the two harvests.