Organ-on-a-chip (OoC) devices simulate human organs within a microenvironment, potentially surpassing traditional preclinical methods and paving the way for innovative treatments. A thorough understanding of the current state of OoC design enables the development of more precise and relevant models that replicate not only the structure of organs but also their intricate cellular interactions and responses to external stimuli. This knowledge facilitates the optimization of biomimetic materials and allows for the real-time simulation of physiological microenvironments. By keeping abreast of new microfabrication techniques, we can explore opportunities to create customized and highly functional OoCs. Objective: To provide a comprehensive overview of microphysiological platform designs. Methods: This systematic review was registered in PROSPERO under the number CRD42022352569. We adhered to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. The eligibility criteria included studies utilizing human tissue, either primary or secondary lineage cells. Results: A total of 9.790 papers were retrieved from the Scopus, Embase, IEEE and Web of Science databases. After removing duplicates and applying a 10-year publication filter, 3.150 articles were screened by title and Full-text analyses were then performed. Eighteen studies met the eligibility criteria and were included in this systematic review. In this review, we examine the principles of OoC design, focusing on structure, dimensions, cell culturing options and manufacturing techniques. We also examine recent advances and future prospects in the field. Conclusions: Microphysiological devices in health research can facilitate drug discovery and improve our understanding of human physiology. They contribute to more ethical research by reducing the number of animals used in experiments.

Purpose: To identify the effectiveness of protocols in the functional rehabilitation process in the postoperative treatment of the hip. Methods: This is a systematic review study, composed of 4 blind and independent evaluators divided into three schematic phases. For the construction of this manuscript, the Recommendation Preferred Reporting Items for Systematic Reviews and Meta-Analyzes was applied. The descriptors used were: "rehabilitation", "physical therapy", "arthroplasty" and "hip" using the Boolean operator “AND” between each of them in the indexing bases. Results: Initially, 136 studies were found in LILACS, MEDLINE, MEDCARIB, PAHO-IRIS and WHOLIS, 99 studies in MEDLINE and 08 articles in PEDro. After reading the abstracts, 13 studies were selected, which were carefully analyzed. Discussion: Among the thirteen selected articles, the main clinical effects observed in the evaluated protocols were: improved functionality, quality of life, reduced pain, increased muscle strength. Conclusion: The functional rehabilitation of postoperative hip arthroplasty was significant in protocols that adopted early physical therapy intervention. These protocols resulted in rapid improvement in functionality, reduction of pain and quality of life. These improvements were also seen in protocols applied at home.

The study investigates the efficacy of a bioinspired Particle Swarm Optimization (PSO) approach for PID controller tuning in Radiofrequency Ablation (RFA) for liver tumors. Ex-vivo experiments were conducted, yielding a 9^thorder continuous-time transfer function. PSO was applied to optimize PID parameters, achieving outstanding simulation results: 0.605% overshoot, 0.314 seconds rise time, and 2.87 seconds settling time for a unit step input. Statistical analysis of 19 simulations revealed PID gains:Kp(mean: 5.86, variance: 4.22, standard deviation: 2.05),Ki(mean: 9.89, variance: 0.048, standard deviation: 0.22),Kd(mean: 0.57, variance: 0.021, standard deviation: 0.14) and ANOVA analysis for the 19 experiments yielded a p-value ≪ 0.05. The bioinspired PSO-based PID controller demonstrated remarkable potential in mitigating roll-off effects during RFA, reducing the risk of incomplete tumor ablation. These findings have significant implications for improving clinical outcomes in hepatocellular carcinoma management, including reduced recurrence rates and minimized collateral damage. The PSO-based PID tuning strategy offers a practical solution to enhance RFA effectiveness, contributing to the advancement of radiofrequency ablation techniques.

Background: The impact of the pandemic caused by the coronavirus (SARS-CoV-2), causing the disease COVID-19, has brought losses to the world in terms of deaths, economic and health problems. The expected return of the public to activities adapted to the new health situation led to discussions about the use of vaccination and its effects. However, the demand for proof of vaccination showed how inconsistent, unregistered, and uncontrolled this health process is with current technologies. Despite the proven effectiveness of vaccines in reducing infection rates, mortality, and morbidity, there are still doubts about their use in preventing certain infections and injuries, as well as the use of digital medical records for identification at public events and disease prevention. Therefore, this review aims to analyze the use of digital immunization cards in disease prevention in general. Methods: A systematic review of Science, PubMed/MEDLINE, LILACS /BSV, CINALH, and IEEE and Xplore was performed using PRISMA guidelines. The authors summarized the studies conducted over the last decade on the impacts of prophylaxis by control through immunization cards. Studies were selected using the following terms: Vaccination; Mobile Applications; Health Smarts Cards; Immunization Programs; Vaccination Coverage. For data analysis, we used Mendeley, Excel, RStudio, and Bibliometrix software among others. Results: A total of 1828 publications were found. After applying eligibility criteria (Articles published in Portuguese, Spanish or English in the last 10 years). Studies that only dealt with paper or physical records were excluded, as well as studies that were not linked to their country’s health Department, as a possibility of bias exists with these types of information). After removing duplicates and applying filters 1 and 2, we included 18 studies in this review. This resulted in 18 papers that met our priori inclusion criteria; it was found that the most relevant sources were from the databases of the Institute of Electrical and Electronics Engineers (IEEE). Conclusions: Considering the selected studies, we found that scientific evidence and epidemiological surveillance are essential tools to characterize the efficiency and effectiveness of immunization passport protection intervention and to ethically justify them. Technological development of digital vaccine passports can assist in vaccination programs and positively impact disease prophylaxis.

Biomaterials interact with biological systems, influencing their responses. Different types of polymers—both natural and synthetic—are widely used in biomedical engineering, among a plethora of healthcare applications, to promote tissue regeneration. The natural rubber latex extracted from Hevea brasiliensis is a biopolymer that whose biocompatibility makes it a valuable study object. Its great regenerative properties are largely associated with the fraction FrHB1, which has demonstrated angiogenic and wound-healing potential by inducing blood vessel formation, collagen synthesis, and fibroblast migration—crucial factors for tissue repair. This study aimed to develop scalable methods for extracting and purifying the F1 protein fraction from industrialized natural latex for biomedical applications. We tested two types of industrial latex, bi-centrifuged and pre-vulcanized latex as well as 60% centrifuged natural latex to determine the most effective composition used in subsequent extractions and fractionation steps. Then, we isolated FrHB1 from the pre-vulcanized latex using selective precipitation, ultrafiltration, and affinity chromatography. The yield of the first batch of this serum was 40.62% with protein concentration of 1.52 ± 0.06 mg/mL. The second batch had a yield of 49.74%; however, due to results lying outside the analytical curve, its protein concentration could not be calculated. The yield of the third batch was 57.19%, and its protein concentration was 1.8477 ± 0.033 mg/mL. This approach facilitates large-scale therapeutic applications utilizing a commercially viable and accessible resource. Moreover, these findings highlight industrialized natural latex as a sustainable source of bioactive molecules, contributing to advancements in regenerative medicine and tissue engineering.

Úlceras em pacientes com Diabetes Mellitus são frequentes e consideradas uma das complicações de maior relevância durante a evolução clínica da doença. Analisar a aplicação do light-emitting diode (LED) azul no reparo tecidual de úlcera venosa de membro inferior. Consistiu na aplicação de LED azul, com comprimento de onda em torno de 680 THz e 620 THz no local da ferida, durante 10 minutos, 2 vezes por semana, durante 5 semanas. Foram utilizados instrumentos avaliativos de dor e sintomas neuropáticos, testes de sensibilidade, registro fotográfico diário, mensuração do tamanho das úlceras e índice do tempo de fechamento completo da ferida. Foi observada melhora no reparo tecidual das feridas, atingindo fechamento total em ambos os lados e redução da intensidade de dor referida. O LED como tratamento curativo de úlceras diabéticas crônicas mostrou efetividade na taxa de fechamento total da ferida e melhora de sintomas neuropáticos.