Aiming at the inherent nature and complexity of the influence of in-orbit micro-vibration in the imaging quality of segmented telescopes, a dynamic full-link opto-mechanical integration analysis method is proposed. The method is based on the measured micro-vibration signals of the infrared refrigerator, using the finite element method to perform the transient response analysis of the opto-mechanical system in Patran/Nastran software. The interface tool is written by Matlab to achieve the calculation of rigid body displacement and real-time data interaction with Zemax. The results show that when the working wavelength is 1 μm, the optical system has a wavefront error Root-Mean-Square value of less than 0.071λ in 4 s. Evaluating the effect of micro-vibration on the imaging quality of the system in terms of the peak ratio of the point spread function. When the exposure time was 2 s, the ratio maximum values of 0.4628 and 0.6207 were reached for the X-axis and Y-axis, respectively. The method provides an important reference basis for the evaluation of imaging quality of an optical system under micro-vibration environment with a long exposure time.