The AP2/ERF family of transcription factors is one of the most conserved and important transcription factor families, and it is ubiquitous in plants. It plays an essential role in plant morphogenesis, molecular mechanisms of stress responses, hormone signaling pathways, and synthesis of secondary metabolites. FaTINY2 was cloned from the octaploid strawberry Fragaria × ananassa for this investigation. Bioinformatics revealed that the protein possesses a conserved AP2 domain and is localized in the nucleus. When FaTINY2 was expressed in plants, quantitative analysis revealed that the gene was tissue-specific. There are lower contents of reactive oxygen species (ROS) and malondialdehyde (MDA), higher contents of proline, chlorophyll, and higher activities of catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD) in transgenic Arabidopsis thaliana than wild type (WT) and unload line (UL) plants under cold and salt stress. FaTINY2 plays a role in enhancing stress tolerance by regulating a few genes linked to the stress response. The findings of this study were that FaTINY2 transgenic Arabidopsis thaliana plants were more tolerant to salt and cold than WT and UL plants. In addition to offering a theoretical reference for strawberry production under stress, this research established a groundwork for exploration into the molecular mechanisms in which strawberries respond to cold and high salt stress.