Background and Objectives: The plant Muscari Mill. is employed in both raw and cooked forms for the treatment of gastric diseases, as an expectorant, and for the treatment of warts and the enhancement of urine. A review of the scientific literature revealed no studies investigating the effect of Muscari neglectum (MN) water extract on gastric diseases. The objective of this study was to determine the effect of a water extract of the MN plant on indomethacin-induced gastric ulcer in rats, using a series of biochemical (SOD, CAT, GSH and MDA levels) and histopathological parameters. Methods: 60 male Sprague Dawley rats were utilized for the purposes of evaluating the acute toxicity and gastric ulcer models, with a total of 36 rats employed for these experiments (n = 6). The rats were divided into six groups: intact; indomethacin; famotidine; indomethacin and MN (100, 200, 400 mg/kg). Results: The Gastric tissue examinations at biochemical, macroscopic and pathological levels showed that MN extracts effectively prevented indo-methacin-induced gastric mucosal damage. The 400 mg/kg dose exhibited the most effective antiulcer effect, with a 69% protective efficacy. This dose caused an increase in the SOD, CAT and GSH levels and a decrease in the MDA levels compared to the IND group. Furthermore, an LC-MS/MS analysis was conducted on the water extract of MN, resulting in the identification of 14 phenolic compounds. Conclusions: Biochemical analyses and histopathological examinations demonstrated that the water extract of MN exhibited a beneficial protective effect against gastric ulceration due to its high antioxidant content.

En tr This experiment was conducted to evaluate the effects of dietary vitamin E (α-tocopherol acetate) and selenium (selenomethionine) and a combination of the two, on the activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT) and malondialdehyde (MDA) levels in serum, egg yolk and egg white of laying hens. Ninety-six white Lohman laying hens aged of 24 weeks were randomly divided into 4 groups. The groups were fed with the diets that consisted of basal diet (2770 kcal/kg metabolic energy and 17 % crude protein) (Control), basal diet + 250 mg / kg Vit-E (Trial-1), basal diet + 0.9 mg/ kg Se (Trial-2) and basal diet + 250 mg / kg Vit-E + 0.9 mg/ kg Se (Trial-3) respectively for 12 weeks. It was found out that when the treatment and control groups were compared in terms of enzymes in serum and egg samples while SOD, CAT and GSH-Px activities increased (p<0.05) and the levels of MDA decreased in the samples of treatment groups (p<0.05). The results of the present study, is recommended that organic selenium and vitamin E supplemental of laying hen diets, alone or together, increased activites of antioxidan enzymes and decreased MDA concentrations in serum and egg. This experiment was conducted to evaluate the effects of dietary vitamin E (α-tocopherol acetate) and selenium (selenomethionine) and a combination of the two, on the activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT) and malondialdehyde (MDA) levels in serum, egg yolk and egg white of laying hens. Ninety-six white Lohman laying hens aged of 24 weeks were randomly divided into 4 groups. The groups were fed with the diets that consisted of basal diet (2770 kcal/kg metabolic energy and 17 % crude protein) (Control), basal diet + 250 mg / kg Vit-E (Trial-1), basal diet + 0.9 mg/ kg Se (Trial-2) and basal diet + 250 mg / kg Vit-E + 0.9 mg/ kg Se (Trial-3) respectively for 12 weeks. It was found out that when the treatment and control groups were compared in terms of enzymes in serum and egg samples while SOD, CAT and GSH-Px activities increased (p<0.05) and the levels of MDA decreased in the samples of treatment groups (p<0.05). The results of the present study, is recommended that organic selenium and vitamin E supplemental of laying hen diets, alone or together, increased activites of antioxidan enzymes and decreased MDA concentrations in serum and egg.

En tr This experiment was conducted to evaluate the effects of dietary vitamin E (Vit-E, α-tocopherol acetate) and selenium (selenomethionine) and a combination of the two, on the selenium, copper, zinc, iron, calcium and magnesium levels in the serum, yolk and albumen of egg. Ninety-six white Lohman LSL laying hens were randomly divided into 4 equal groups (n=24). The groups were fed with the diets that consisted of basal diet (Control), basal diet + 250 mg / kg Vit-E (Trial- I), basal diet + 0.9 mg/kg Se (Trial-II) and basal diet + 250 mg/kg Vit-E + 0.9 mg/ kg Se (Trial-III) respectively for 12 weeks. Diet and water were provided as ad libitum. At the end of the experiment, samples of the blood and egg were collected and stored at -20 ºC until analyzes. In samples bioelements levels were analyzed by ICP-MS. As a result, the addition of organic selenium and Vit-E alone and in combination to laying hens rations were increased Se, Zn and Fe levels in serum, egg white and egg yolk, Cu levels decreased in serum and egg white, whereas in the egg yolk that the decrease in the group fed with rations supplemented with 0.9 mg/kg Se and the levels of Ca and Mg remained unaffected when the experimental and control groups. In conclusion, it is suggested that organic selenium and vitamin E be added alone and in combination to egg hen rations in order to increase egg quality and prolong shelf life. Bu çalışmada, yumurta tavuğu rasyonlarına vitamin E (Vit-E, α-tokoferol asetat) ve selenyumun (selenometiyonin) tek başına ve kombine olarak ilavesinin serum, yumurta akı ve yumurta sarısında selenyum, bakır, çinko, demir, kalsiyum ve magnezyum düzeyleri üzerine etkilerinin araştırılması amaçlanmıştır. Çalışmada, 96 adet beyaz Lohman yumurta tavuğu, eşit sayıda (n=24) 4 gruba ayrıldı. Gruplar sırasıyla bazal yem (Kontrol), bazal yem + 250 mg/kg Vit-E (D-I), bazal yem + 0.9 mg/ kg Se (DII) ve bazal yem + 250 mg/kg Vit-E+ 0.9 mg/ kg Se (D-III) içeren rasyonlar ve su ile 12 hafta, adlibitum olarak beslendi. Deneme sonunda, her gruptan alınan kan ve yumurtada biyoelement düzeyleri, ICP-MS cihazı ile analiz edildi. Deneme gruplarının serum, yumurta sarısı ve yumurta akında Se, Zn ve Fe düzeyleri kontrol grubu ile karşılaştırıldığında istatistiki olarak önemli derecede artarken (P<0.05), Cu düzeyleri serum ve yumurta akında önemli derecede azaldığı (P<0.05), yumurta sarısında ise 0.9 mg/kg Se ilave edilen rasyonlar ile beslenen gruptaki azalmanın önemli (P<0.05) olduğu, Ca ve Mg düzeylerinin ise etkilenmediği bulunmuştur. Sonuç olarak, organik selenyumun ve vitamin E’nin yumurtanın kalitesinin artırılmasında ve raf ömrünün uzatılmasında, yumurta tavuğu rasyonlarına tek başına ve kombinasyonu şeklinde ilave edilmesi önerilmektedir.

In the study, the protective effects of boron against paracetamol (PR)-induced toxicity in rat testicular tissue were investigated using various biochemical parameters. Rats were categorized into five groups and administered 50 and 100 mg/kg of boron (sodium pentaborate) orally for six days, followed by a single dosage of 1 g/kg of paracetamol to induce toxicity. Testicular tissues were assessed using ELISA and levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), cysteine-aspartic acid protease (Caspase-3), malondialdehyde (MDA), reduced glutathione (GSH), tumor necrosis factor-α (TNF-α), and interleukin-1 beta (IL-1β) were assessed. A significant decrease in SOD, CAT, GPx activities and GSH levels and an increase in MDA levels were observed in the PR group. Boron (B) treatment was found to increase antioxidant levels while decreasing lipid peroxidation, inflammation and apoptosis markers in paracetamol-induced testicular toxicity (p

Tr en Bu çalışma, diyetsel vitamini E (a-tokoferol asetat), organik selenyum (selenomethionine) ve bunların kombinasyonlarının yumurta tavuklarının bazı dokularında bazı biyoelementlerin düzeyleri üzerine etkilerini değerlendirmek amacıyla yapıldı. Çalışmada, 96 adet beyaz Lohman yumurta tavuğu, eşit sayıda 4 gruba ayrıldı. Gruplar sırasıyla bazal yem (Kontrol), bazal yem + 250 mg/kg Vit-E (D-I), bazal yem + 0.9 mg/kg Se (DII) ve bazal yem + 250 mg/kg Vit-E+ 0.9 mg/ kg Se (D-III) içeren rasyonlarla beslendi. Deneme sonunda, her gruptan 12 hayvan kesildi, belirtilen dokular alındı, analiz edilinceye kadar -20 ºC'de saklandı ve biyoelement düzeyleri ICP-MS ile analiz edildi. Deneme gruplarının dokularındaki Se, Zn ve Fe düzeyleri kontrol grubuna göre önemli derecede artarken (P<0.05), Cu düzeylerinin azaldığı (P<0.05), Ca ve Mg düzeylerinin ise muameleden etkilenmediği bulundu. Organik selenyumun ve vitamin E’nin, dokularda biyoelement düzeylerindeki değişmelerin oluşturacağı antagonist ve sinerjik etkilerinin dikkate alınması önerilmektedir. This experiment was conducted to evaluate the effects of dietary vitamin E (α-tocopherol acetate), organic selenium (selenomethionine) and their combination on some bioelements in some tissues of laying hens. Ninety-six white Lohman LSL laying hens aged of 24 weeks were randomly divided into 4 equal groups. The groups were fed with the diets consisting of basal diet (Control), basal diet + 250 mg/kg Vit-E (Trial-I), basal diet + 0.9mg/kg Se (Trial-II) and basal diet + 250 mg/kg Vit-E + 0,9 mg/kg Se (Trial-III), respectively. At the end of the experiment, 12 laying hens from each group were sacrificed, and aforementioned tissues were collected and stored at -20 ºC until analyzes. In tissue samples bioelements levels were determined by ICP-MS. It was found out that when the treatment and control groups were compared in terms of bioelements in some important tissues while Se, Zn and Fe levels increased (P<0.05) and the levels of Cu decreased in the tissues of treatment groups (P<0.05). In addition, the levels of Ca and Mg were not affected by treatment in present study. Organic selenium and vitamin E, consideration of antagonist and synergistic effects of bioelement levels changes in tissues.