This study aimed to examine the relationships between isometric mid-thigh pull maximal force (IMTPF), sprint mechanics, and performance. Fifteen national-level track and field athletes (sprinters and hurdlers) performed three maximal-effort isometric mid-thigh pulls on a force plate and two 30 m sprints. The IMTPF, the sprint mechanical variables (theoretical maximum horizontal force (F₀), velocity (v₀), and power (P_max)), as well as the sprint performance data at 5 m distance intervals, were collected. Pearson’s product–moment correlation analysis revealed large linear associations between IMTPF and v₀ (r = 0.65, R² = 0.42, p = 0.009), as well as negative linear relationships between IMTPF and sprint times of 15 m (r = −0.53, R² = 0.28, p = 0.043), 20 m (r = −0.55, R² = 0.30, p = 0.033), 25 m (r = −0.57, R² = 0.33, p = 0.025), and 30 m (r = −0.60, R² = 0.36, p = 0.019). The F₀, P_max, and sprint times to 5 m and 10 m were not significantly correlated with the IMTPF (p < 0.05). The study results highlight that during the late acceleration phase (>15 m), the capacity to generate horizontal force at high running velocities is related to the ability to develop maximal force during isometric contractions.