In this paper, a new tuning method is proposed, based on the desired dynamics equation (DDE) and the generalized frequency method (GFM), for a two-degree-of-freedom proportional-integral-derivative (PID) controller. The DDE method builds a quantitative relationship between the performance and the two-degree-of-freedom PID controller parameters and guarantees the desired dynamic, but it cannot guarantee the stability margin. So, we have developed the proposed tuning method, which guarantees not only the desired dynamic but also the stability margin. Based on the DDE and the GFM, several simple formulas are deduced to calculate directly the controller parameters. In addition, it performs almost no overshooting setpoint response. Compared with Panagopoulos’ method, the proposed methodology is proven to be effective.