Immobilization of yeast cells represents a significant advance in alcoholic fermentation. Compared to traditional methods that rely on the use of free cells, immobilized systems enable higher cell density, easier separation and reuse of biocatalysts, and improved fermentation control, all while maintaining cellular activity. The choice of immobilization material plays a key role in performance. Natural polymers such as alginate provide biocompatibility, but the main drawback is their insufficient mechanical strength. On the other hand, synthetic polymers offer greater durability but raise concerns regarding food safety and cost. Three-dimensional (3D) bioprinting is emerging as a promising solution, enabling the design of structural, customizable matrices with precise cell positioning and tunable physical properties. Traditional materials are undergoing reengineering as bioinks, while new synthetic and hybrid materials are being developed to overcome the limitations of conventional carriers. These innovations combine biocompatibility with mechanical stability and functional adaptability for industrial use. Although the application of 3D bioprinting to produce such carriers has shown promising progress, challenges remain in scalability, process integration, and long-term stability under industrial fermentation conditions. For these reasons, continued interdisciplinary research is necessary to further develop advanced techniques for immobilizing yeast cells for use in alcoholic fermentation.

Fermentable oligo-, di-, and monosaccharides as well as polyols (FODMAPs) came into focus following recent clinical studies confirming that they worsen the symptoms of several gastrointestinal disorders suffered by 40% of the general population. Currently; only the low-FODMAP diet is a valuable strategy to help relieve IBS symptoms; however; it is only a temporary solution due to the nutritional deficiency caused by avoiding high-FODMAP foods. At the same time; bakery products are an important part of the human diet worldwide and the key contributors to the high intake of FODMAPs; especially in their wholegrain form. Previous research has shown that reducing FODMAPs content has negative effects on the structures of dough and bread; as well as on sensory quality. Our innovative low-FODMAP wholegrain bakery products provide a unique solution for achieving a high-dietary-fiber intake without compromising the sensory appeal. The novelty of our work is that these experiments were the first to be performed based on known but unexploited facts about the superiority of the baker’s yeast enzymatic complex. A crucial reduction in FODMAP content (by more than 75%) was achieved via a simple alteration to the bread formulation (6% baker’s yeast and the addition of baking powder) and key process parameter values (40 °C and 60 min dough fermentation time) in conventional breadmaking technology.

Fermentable oligosaccharides, di- and monosaccharides, and polyols defined as FODMAPs readily trigger the symptoms of irritable bowel syndrome (IBS), which affects up to 23% of the population, through several mechanisms. A low-FODMAP diet is a short-term solution due to significant nutrient deficiencies, especially in dietary fibre (DF). IBS patients must avoid cereals, especially wholegrain cereals such as wheat and rye, which are an important natural source of DF and therefore FODMAPs (part of soluble DF). This study is the first of its kind to employ biotechnological tools for the creation of wholegrain low-FODMAP cookies and crackers based on wholegrain wheat and rye flours with high FODMAP contents. Endogenous enzymes activated via prolonged dough resting and exogenously activated enzymes originating from chicory extract, wheat malt, and baker’s yeast were employed. The prolonged dough resting time and the addition of wheat malt reduced the FODMAP content in the wholegrain wheat and rye cookies by 46% and 99.5%, respectively. The best result was achieved in the wholegrain wheat crackers, with a FODMAP content reduction of 59.3% based on the combination of a prolonged dough resting time and the addition of wheat malt and baker’s yeast. In the wholegrain rye crackers, a prolonged resting time alone was sufficient to achieve an 83.6% reduction in the total oligosaccharide content.