An analysis of the temperature regime and air humidity was carried out in ecotonal pine-broadleaf forestsof the suballianceTilio cordatae–Pinenion sylvestris on the Ufa Plateau (UP) and in the central part of the Southern Urals (MFZ – mountain forest zone of the Republic of Bashkortostan (RB)) based on data from temperature and humidity sensors (2022–2023) and the ERA5-Land reanalysis. The mean daily air temperature on the UP was on average 1,24°C higher than in the MFZ according to logger data and 2,3°C higher according to the ERA5-Land reanalysis. A high correlation between logger observations and reanalysis data was detected (R = 0,97–0,98). However, the air temperature measured by sensors was on average higher than the ERA5-Land estimates by 1,7°C on the UP and by 0,67°C in the MFZ. During extreme periods, discrepancies reached up to 13°C, which is associated with the buffering role of the forest canopy, the influence of the snow cover, and microlandscape features such as slope exposure and steepness. Relative air humidity at both sites showed very similar average values (74,3% on the UP and 74,0% in the MFZ) and a high synchrony in seasonal dynamics. The highest values were observed in November (up to 90%), while the lowest values occurred in May (about 50%). During the cold season, humidity remained high due to low temperatures and stable snow cover, whereas in spring its variability increased. Soil temperature in winter remained close to 0°C, indicating the strong insulating effect of the snow cover. In spring and summer, soil on the UP warmed more rapidly and reached higher values (up to +17…+18°C) than in the MFZ (up to +15…+16°C). These differences are explained by variations in slope exposure, relief, and conditions of solar radiation input. The obtained results demonstrate the important role of microlandscape factors and forest canopy in shaping the microclimatic regime of forest ecosystems. The obtained results highlight the key role of local factors (microrelief, forest canopy) in the formation of microclimate, which must be taken into account when predicting the response of forest ecosystems to global climate change, especially in ecotone communities sensitive to climate fluctuations.