The Plackett-Burman Design (PBD) was applied to study fresh water microalgae cultivation using Chlorella sp. TISTR 8411 to select the influential nutrient factors for biomass and lipid production. The PBD for 13 trials from 11 nutrient factors with 3 levels was studied in the mixotrophic cultivation at 28 ⁰C under 16:8 light and dark photoperiods over 7 days of cultivation time. Two influential factors were chosen as glucose and cobalt chloride hexahydrate to further design via Box-Behnken Design (BBD) in order to optimize the cultivation of this microalgae for biodiesel production. The 17 trials of 3 factors and 3 levels of BBD experimental design technique were applied with varying factors of glucose (20-40 g/L), cobalt chloride hexahydrate (0.01-0.04 mg/L) and light intensity (4,500-7,500 Lux) under 16:8 light and dark photoperiods over 7 days of cultivation time at 28 ⁰C. Result showed that Chlorella sp. TISTR 8411 cultivation yield 0.52 g/L biomass and 0.31 g/L lipid production resulting in approximately 60% of lipid production when cultivated in 20.05 g/L glucose, 0.04 mg/L CoCl26H2O under light intensity of 4,614 Lux with the supplementation of 4.38 g/L NaHCO3 coupled with 1 g/L of both NaNO3 and KH2PO4. Under statically mixotrophic cultivation, result indicated that Chlorella sp. TISTR 8411 had potential to produce high lipid content for biodiesel application and biomass production for nutraceutical application. Further experiment with the longer cultivation period up to 2 weeks would implement not only for monitoring the growth kinetics but also evaluating the suitable type of fatty acid production.

The accumulation lipid from oleaginous microorganisms is recognized as a second generation fuel. Biooil is known to as intracellular product of oily yeast utilizing various carbon substrates and converting different quantities of lipids in the form of triacylglycerols. This second generation fuel can be used to make biodiesel via a transesterification process. This study investigated the morphological characteristics of eight strains of Thai oleaginous yeasts via microscopy and analyzed the fatty acid profiling of yeasts cultured in three carbon sources: glucose, sugar cane molasses and crude glycerol in order to estimate biodiesel properties. To approach this goal, batch fermentations were used to culture eight yeast strains,Rhodosporidium toruloidesTISTR 5123, TISTR 5154, TISTR 5149,Yarrowia lipolyticaTISTR 5054, TISTR 5151, TISTR 5621,Rhodotorula glutinisTISTR 5159 andRhodotorula graminisTISTR 5124 for 96 h under 30°C at 250 rpm. Result revealed that eight yeast strains contained significant amounts of fatty acids and lipids and accumulated mainly palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C 18:1) and linoleic acid (C18:2), and they are suitable for the production of biodiesel. Fatty acid productions and profiles indicated that these yeast strains can be potentially used as the triacylglycerols producers for biodiesel production.

Nonthermal processing methods are attracted by many food and beverage industry because it can kill the microbial contamination under mild temperatures used in thermal processing; They can sustain flavours, essential nutrients, and vitamins undergo minimal changes. The objective of this research was to evaluate nonthermal processing as the high current impulse generator (HCIG) and investigate the reduction the contaminated microorganism in coconut juice under the batch and continuous treatment of HCIG. The physical, biochemical and nutritional changes of treated coconut juice were also investigated. The application of the direct electricity through cathode of high current impulse generator (HCIG) in 1,170 chambers contained the contaminated coconut juice. Significantly reduction bothSaccharomyces cerevisiaeandEscherichia colias 5-log were found when treatments were applied with impulses at 5.17 kA. Comparison of nutritional value of non-thermal processes before and after high current impulse was showed no significant differences between main nutritional values and free amino acid. For the continuous HCIG treatment under the treatment of 5.17 kA current with 9, 15 and 30 pulses with 5 L coconut juice at the flow rate of 1 L/min, results from initial concentration at 1.41 × 10⁵CFU/mL showed thatS. cerevisiaereductions were found 78%, 66% and 96% as increasing number of pulses as 9, 15 and 30 pulses, respectively. The increment of microbial reduction with the increasing number of pulses was also detected as 78 %, 82 % and 96 % from 1.11 × 10⁵CFU/mlE. coli.Results revealed that the microbial reduction with HCIG under batch treatment were successful preserved the nutritional components of the coconut juice without significant physicochemical changes.