Germination response to fluctuating temperatures is a mechanism by which seeds detect gaps in vegetation canopies and depth of burial in soil, and it is very important for plants. Thus, studies on the effect of fluctuating temperature on germination at the community level are valuable for understanding community structure and biodiversity maintenance. We determined the effects of two alternating temperatures (5/25°C and 10/20°C) and one constant temperature (15°C) on seed germination of 445 species in a grassland community on the eastern Tibet Plateau. Seed mass was determined for each species, and data on habitat, type of life cycle, altitudinal distribution and functional group (graminoids or forbs) were obtained from the literature. Taking all species into account, alternating temperatures increased germination percentages regardless of amplitude. Overall, species growing in disturbed ground showed a significant germination response to temperature fluctuation, but those living in Alpine/subalpine meadow, forest margin /scrub, marshland and dry sunny slope habitats did not. Species distributed only at high elevations (>2000m) did not show a significant germination response to temperature fluctuation, whereas those occurring at both high and low elevations had a significant positive response. Germination of annuals/biennials was significantly promoted by 5/25°C, but not by 10/20°C, whereas germination of perennials was significantly promoted by both 5/25°C and 10/20°C. Small-seeded species were more likely than large-seeded species to respond positively to fluctuating temperatures. Germination of forbs had a positive response to temperature fluctuation, but germination of graminoids did not. Regeneration ability by seeds for about 36% of the species studied in the grassland can be increased by temperature fluctuation. The differential response among species to alternating vs. constant temperatures helps maintain community structure and biodiversity. A positive germination response to temperature fluctuation can partly explain why there are more forbs in degraded meadows.

Summary: Within a community, species may germinate at different times so as to mitigate competition and to take advantage of different aspects of the seasonal environment (temporal niche differentiation). We illustrated a hypothesis of the combined effects of abiotic and biotic competitive factors on germination timing and the subsequent upscale effects on community assembly. We estimated the germination timing (GT) for 476 angiosperm species of the eastern Tibetan Plateau grasslands under two light treatments in the field: high (i.e. natural) light and low light. We also measured the shift in germination timing (SGT) across treatments for all species. Furthermore, we used phylogenetic comparative methods to test if GT and SGT were associated with seed mass, an important factor in competitive interactions. We found a significant positive correlation between GT and seed mass in both light treatments. Additionally, small seeds (early germinating seeds) tended to germinate later and large seeds (late germinating seeds) tended to germinate earlier under low light vs high light conditions. Low light availability can reduce temporal niche differentiation by increasing the overlap in germination time between small and large seeds. In turn, reduced temporal niche differentiation may increase competition in the process of community assembly.

• Premise of the study: Little is known about the effect of environmental conditions on seed dormancy break at the community level or how it could be affected by climate change. This study tested the effects of storage conditions on germination of 489 species from high‐elevation natural grasslands on the Tibet Plateau. • Methods: We stored seeds in dry cold, dry warm, and wet cold environments to test the effect of these conditions on germination. Germination responses were classified with the use of cluster analysis. The effect of phylogeny on germination response to storage conditions was determined. • Key results: Compared with results of wet cold conditions, storing seeds at dry warm or dry cold conditions decreased the mean community germination percentage by 17.93% and 16.07%, respectively. Storing seeds at dry warm vs. dry cold conditions decreased the community mean germination percentage by 4.61%. The germination response to moisture conditions during storage showed significant phylogenetic patterns, whereas the germination response to storage temperature did not. On the basis of the germinability of seeds of the same species stored under different conditions, germination may increase, decrease, or stay the same. • Conclusions: Within the high‐elevation Tibetan grassland community, the different responses in dormancy breaking and germination behavior to the same storage conditions may have implications for understanding how this community might respond to climate change. In particular, some species may increase in the community, whereas others may decrease or remain the same. However, the potential for such change can be detected only by studying the responses of many species.