The Smad7 protein is negative regulator of the TGF-β signaling pathway, which is upregulated in patients with breast cancer. miRNAs regulate proteins expressions by arresting or degrading the mRNAs. The purpose of this work is to identify a miRNAs profile that regulates the expression of the mRNA coding for Smad7 in breast cancer using the data from patients with breast cancer obtained from the Cancer Genome Atlas Project. We develop an automatic search method based on genetic algorithms to find a predictive model based on deep neural networks (DNN) which fit the set of biological data and apply the Olden algorithm to identify the relative importance of each miRNAs. A computational model of non-linear regression is shown, based on deep neural networks that predict the regulation given by the miRNA target transcripts mRNA coding for Smad7 protein in patients with breast cancer, with R2 of 0.99 is shown and MSE of 0.00001. In addition, the model is validated with the results in vivo and in vitro experiments reported in the literature. The set of miRNAs hsa-mir-146a, hsa-mir-93, hsa-mir-375, hsa-mir-205, hsa-mir-15a, hsa-mir-21, hsa-mir-20a, hsa-mir-503, hsa-mir-29c, hsa-mir-497, hsa-mir-107, hsa-mir-125a, hsa-mir-200c, hsa-mir-212, hsa-mir-429, hsa-mir-34a, hsa-let-7c, hsa-mir-92b, hsa-mir-33a, hsa-mir-15b, hsa-mir-224, hsa-mir-185 and hsa-mir-10b integrate a profile that critically regulates the expression of the mRNA coding for Smad7 in breast cancer. We developed a genetic algorithm to select best features as DNN inputs (miRNAs). The genetic algorithm also builds the best DNN architecture by optimizing the parameters. Although the confirmation of the results by laboratory experiments has not occurred, the results allow suggesting that miRNAs profile could be used as biomarkers or targets in targeted therapies.

Multi-layer perceptron artificial neural networks (MLP-ANNs) were used to predict the concentration of digoxin needed to obtain a cardio-activity of specific biophysical parameters in Tivela stultorum hearts. The inputs of the neural networks were the minimum and maximum values of heart contraction force, the time of ventricular filling, the volume used for dilution, heart rate and weight, volume, length and width of the heart, while the output was the digoxin concentration in dilution necessary to obtain a desired physiological response. ANNs were trained, validated and tested with the dataset of the in vivo experiment results. To select the optimal network, predictions for all the dataset for each configuration of ANNs were made, a maximum 5% relative error for the digoxin concentration was set and the diagnostic accuracy of the predictions made was evaluated. The double-layer perceptron had a barely higher performance than the single-layer perceptron; therefore, both had a good predictive ability. The double-layer perceptron was able to obtain the most accurate predictions of digoxin concentration required in the hearts of T. stultorum using MLP-ANNs.

In recent years, the agriculture sector has undergone a significant digital transformation, integrating artificial intelligence (AI) technologies to harness and analyze the growing volume of data from diverse sources. Machine learning (ML), a powerful branch of AI, has emerged as an essential tool for developing knowledge-based agricultural systems. Grapevine red blotch disease (GRBD) and grapevine leafroll disease (GLD) are viral infections that severely impact grapevine productivity and longevity, leading to considerable economic losses worldwide. Conventional diagnostic methods for these diseases are costly and time-consuming. To address this, ML-based technologies have been increasingly adopted by researchers for early detection by analyzing the foliar symptoms linked to viral infections. This study focused on detecting GRBD and GLD symptoms using Convolutional Neural Networks (CNNs) in computer vision. YOLOv5 outperformed the other deep learning (DL) models tested, such as YOLOv3, YOLOv8, and ResNet-50, where it achieved 95.36% Precision, 95.77% Recall, and an F1-score of 95.56%. These metrics underscore the model’s effectiveness at accurately classifying grapevine leaves with and without GRBD and/or GLD symptoms. Furthermore, benchmarking was performed with two edge computer devices, where Jetson NANO obtained the best cost–benefit performance. The findings support YOLOv5 as a reliable tool for early diagnosis, offering potential economic benefits for large-scale agricultural monitoring.