B-cell epitope prediction is crucial for advancing immunology, particularly in vaccine development and antibody-based therapies. Traditional experimental techniques are hindered by high costs, time consumption, and limited scalability, making them unsuitable for large-scale applications. Computational methods provide a promising alternative, enabling high-throughput screening and accurate predictions. However, existing computational approaches often struggle to capture the complexity of protein structures and intricate residue interactions, highlighting the need for more effective models. This study presents GraphEPN, a novel B-cell epitope prediction framework combining a vector quantized variational autoencoder (VQ-VAE) with a graph transformer. The pre-trained VQ-VAE captures both discrete representations of amino acid microenvironments and continuous structural embeddings, providing a comprehensive feature set for downstream tasks. The graph transformer further processes these features to model long-range dependencies and interactions. Experimental results demonstrate that GraphEPN outperforms existing methods across multiple datasets, achieving superior prediction accuracy and robustness. This approach underscores the significant potential for applications in immunodiagnostics and vaccine development, merging advanced deep learning-based representation learning with graph-based modeling.

The rapid evolution of unmanned aerial vehicle (UAV) technology and low-altitude economic development have propelled drone applications in critical infrastructure monitoring, particularly in intelligent transportation systems where real-time aerial image processing has emerged as a pressing requirement. We address the pivotal challenge of highway lane extraction from low-altitude UAV perspectives by applying a novel three-stage framework. This framework consists of (1) a deep learning-based semantic segmentation module that employs an enhanced STDC network with boundary-aware loss for precise detection of roads and lane markings; (2) an optimized polynomial fitting algorithm incorporating iterative classification and adaptive error thresholds to achieve robust lane marking consolidation; and (3) a global optimization module designed for context-aware lane generation. Our methodology demonstrates superior performance with 94.11% F1-score and 93.84% IoU, effectively bridging the technical gap in UAV-based lane extraction while establishing a reliable foundation for advanced traffic monitoring applications.