The aim of this study was to determine the effects of dietary vibroactivated clinoptilolite supplementation on the intramammary microbiological findings in dairy cows, causative agents of potential intramammary infection, and their sensitivity to antibiotics. Cows (n = 78) were randomly divided into two groups: CPL-treated group that received clinoptilolite (CPL) in-feed (n = 38), i.e., 50 g natural powdered zeolite CPL, twice daily from the seventh month of pregnancy to 75 days after calving, and the control group (CON) of untreated cows (n = 40). Milk samples were taken from each cow on days 7, 25, 45, and 75 postpartum. The following causative pathogens were isolated in 86 udder quarters: Staphylococcus aureus in 5.81% of positive samples, Staphylococcus spp. 9.32%, coagulase-negative Staphylococcus (CNS) 22.09%, Streptococcus uberis 13.95%, Streptococcus agalactiae 1.16%, Streptococcus sp. 3.49%, Escherichia coli 8.13%, Enterococcus spp. 6.98%, Corynebacterium spp. 11.63%, Pasteurella sp. 10.47%, Serratia spp. 2.33%, and Arcanobacterium pyogenes, Citrobacter sp., Prototheca sp., and yeasts each in 1.16% of samples. Additionally, 3.87% of environmental microflora samples (n = 47) and 89.06% of udder samples (n = 1083) were bacteriologically negative. The most effective antibiotics were cefoperazone and amoxicillin-clavulanic acid, while cloxacillin and tetracycline were the least effective antibiotics in both groups. In the clinoptilolite supplemented (CPL) group (n = 38) of 14 cows, nine causative agents of mastitis were isolated in 27 quarters, while in the control (CON) group (n = 40) of 24 cows, 13 causative agents of mastitis were isolated in 59 quarters. Cows from the CON group had a 1.96 times higher risk of intramammary infection than cows from the CPL group during the observation period (odds ratio = 1.96, p = 0.0031; 95% CI = 1.2570–3.0770).

Exotic pet medicine is rapidly evolving, with reptiles becoming increasingly popular pet animals. Yet, there are only a few literature reports on veterinary perception of reptiles kept as pets. The aim of the study was to assess opinions and knowledge of the Croatian veterinarians-to-be about pet reptiles and their welfare. The questionnaire survey was conducted in the academic year 2019–2020 and included students of all six years of the integrated undergraduate and graduate study at the Faculty of Veterinary Medicine, University of Zagreb. First-year students were surveyed twice, before and after having attended the compulsory course on animal welfare. Questionnaire statements were 5-point Likert scale questions, requiring the students to express their opinions about turtles, lizards and snakes as pets, issues related to their welfare, risks they pose to the health and safety of humans, other animals and the environment, and their self-reported knowledge about pet reptiles. Although expressing higher opinions after having attended the course on animal welfare, first-year student responses remained neutral to most of the statements. Such a trend continued until the end of the study. Student responses revealed that they were uncertain about their knowledge of reptiles as pets, considering different educational areas observed. Study results emphasised the need of alterations in veterinary curriculum and additional student education in reptile medicine. The results obtained have broad implications involving not only the welfare of pet reptiles in clinical practice and elsewhere but also the health and safety of humans and other animals, as well as environmental protection.

Air quality in zoo premises is insufficiently investigated, yet scientific interest is growing. In these places, air pollutants such as microorganisms may represent health risk for both animals and exposed people. Thus, maintaining good air quality is crucial for ensuring long-term sustainability of zoo operations. The present study aimed to assess bacterial air contamination level and microclimate conditions in Zagreb Zoo. Measurements were performed in the area for visitors and employees inside four premises, i.e., Monkey House, Tropical House, Rainy Africa, and Bird House, as well as outside the premises in the summer–autumn period. Total number of bacteria in the premises ranged from 4.50 × 10² to 3.70 × 10⁴ CFU/m³, and number of Gram-negative bacteria ranged from 0 to 5.50 × 10² CFU/m³. Total number of bacteria in outdoor air ranged from 50 to 8.50 × 10² CFU/m³, and number of Gram-negative bacteria ranged from 0 to 50 CFU/m³. Total number of bacteria was significantly higher in the Rainy Africa and Bird House as compared to the Monkey House and Tropical House, yielding a significant positive correlation with the number of Gram-negative bacteria in the premises. Total number of bacteria in outdoor air was significantly lower as compared to all investigated premises, except for the Monkey House, yielding a significant positive correlation with the number of Gram-negative bacteria outdoors. Air temperature showed a significant negative correlation with both total number of bacteria and number of Gram-negative bacteria, and airflow rate showed a significant negative correlation with total number of bacteria in the premises. Air temperature showed a significant positive correlation and relative humidity significant negative correlation with total number of bacteria outdoors. Study results can serve in the development of air quality standards in zoos, contributing to finding effective strategies to mitigate health risk from bioaerosols, with implications for occupational and public health, and overall zoo sustainability.