Adenocaulon himalaicum is widely distributed across Asia. In its early growth stages, A. himalaicum is traditionally consumed as a food source in Korea. Although previous research has identified the presence of bioactive compounds in A. himalaicum extract, suggesting its potential as a medicinal resource, the phytochemical profile of A. himalaicum extract has not been extensively determined. This investigation aimed to identify the phytochemicals present in the true leaf and cotyledon of A. himalaicum (TLA and CLA, respectively) and evaluate their radical-scavenging activity. By performing LC-MS/MS and HPLC, varying amounts of isochlorogenic acid A, cryptochlorogenic acid, isochlorogenic acid B, rutin, chlorogenic acid, hyperin, and neochlorogenic acid were detected in the TLA and CLA extracts. Chlorogenic acid (9.002 mg/g DW), isochlorogenic acid A (28.512 mg/g DW), and isochlorogenic acid B (12.223 mg/g DW) were the most abundant in TLA. TLA exhibited higher phytochemical content (49.737 mg/g DW), total phenolic content (45.51 mg tannic acid equivalent/g extract), and total flavonoid content (16.24 mg quercetin equivalent/g extract) than CLA. Moreover, the radical-scavenging activity of TLA was two times higher than that of CLA. The young leaf of A. himalaicum has a rich phytochemical profile and robust antioxidant activity; hence, it has potential as natural antioxidant sources for human health and valuable pharmacognosy raw materials for pharmaceutical and functional food applications.

This study investigated the extraction and quantification of specific phenolic compounds, including chlorogenic acid and several pyranocoumarin derivatives, from the leaves and roots of Peucedanum japonicum. Using high-performance liquid chromatography, this study aimed to optimize extraction methodologies with different solvents to maximize the yield of bioactive compounds. The extraction process involved meticulous steps, including reflux extraction and solvent evaporation, and the total phenolic content was assessed using a spectrophotometric assay. The results demonstrated that ethanol and methanol were effective in extracting chlorogenic acid, yielding a total phenolic content of up to 47.71 mg/g tannic acid equivalent in MeOH extracts from roots. Conversely, acetone was superior for pyranocoumarin extraction, achieving a total coumarin content exceeding 100 mg/g in root extracts. Notably, pyranocoumarins were found to be more concentrated in the roots compared to leaves, supporting the hypothesis that roots are a reservoir for these bioactive compounds. This study emphasized the critical roles of solvent selections in profiling bioactive compounds from P. japonicum and provided valuable insights for future research into its pharmacological potential. The findings may serve as a foundation for further pharmacological studies, enhancing the understanding of the medicinal properties of P. japonicum in the context of traditional East Asian medicine.

Allium longistylum is a relatively understudied species whose phytochemical composition and biological activities remain largely unexplored. In this study, the first true leaf (FTL) and the second true leaf (STL) of A. longistylum were compared with respect to phenolic composition, antioxidant capacity, antimicrobial activity, and quorum-sensing (QS) inhibition. Total phenolic content (TPC) and total flavonoid content (TFC) were determined spectrophotometrically, while antioxidant activity was evaluated using ABTS and DPPH radical scavenging assays. Antimicrobial and anti-QS activities were assessed against Staphylococcus aureus, Acinetobacter baumannii, and Chromobacterium violaceum. STL exhibited significantly higher TPC and TFC than FTL, consistent with its stronger radical scavenging activity. Both extracts showed moderate antimicrobial activity and reduced violacein production in C. violaceum, indicating interference with QS. UPLC-Q-Orbitrap-ESI-MS/MS profiling tentatively identified several phenolic acids and flavonoid derivatives. HPLC analysis confirmed the presence of selected phenolic compounds, although several prominent peaks in the chromatograms remained unidentified. Many of the compounds detected by UPLC-Q-Orbitrap-ESI-MS/MS and HPLC have previously been reported to exhibit antioxidant, antimicrobial, and anti-QS activities; their presence may therefore contribute to the bioactivities observed in both extracts. However, their contribution to the observed effects remains speculative and requires further validation through targeted isolation and bioactivity testing. The results suggest that A. longistylum is a promising source of phenolic compounds with antioxidant and antimicrobial properties.

1. Atopic dermatitis (AD) is a chronic, relapsing inflammatory skin disorder characterised by pruritus, eczematous lesions, and impaired epidermal barrier function, which affects approximately 20% ...

Sang-Yun Lee · Su-Young Jung · Doo-Hee Lee · Sanghyun Lee. JABC 2025;68:520-30. https://doi.org/10.3839/jabc.2025.065