Three new steroidal alkaloids with an unusual 3α tigloylamide group, named sarchookloides A–C (1–3), were isolated along with four known compounds (4–7) from the roots of Sarcococca hookeriana. Their structures and relative configuration were elucidated on the basis of spectroscopic methods including MS, UV, IR, 1D, and 2D NMR data. The isolated compounds were evaluated for their cytotoxicity against five human cancer cell lines: Hela, A549, MCF-7, SW480, and CEM in vitro. All three amide substituted steroidal alkaloids exhibited significant cytotoxic activities with IC₅₀ values of 1.05–31.83 μM.

C₂₇H₃₉NO, orthorhombic, P2₁2₁2₁ (no. 19), a = 10.5248(7) Å, b = 12.6248(7) Å, c = 16.8913(9) Å, V = 2244.4(2) ų, Z = 4, R_gt (F) = 0.0435, wR_ref (F ²) = 0.1033, T = 173(2) K.

C₂₈H₄₁NO, orthorhombic, P2₁2₁2₁ (no. 19), a = 10.5758(10) Å, b = 12.3659(9) Å, c = 18.0301(16) Å, V = 2358.0(4) ų, Z = 4, R_gt (F) = 0.0524, wR_ref (F ²) = 0.1220, T = 273(2) K.

A phytochemical investigation of the leaves of the medicinal plant Isodon rubescens led to the isolation of the two new degraded abietane lactone diterpenoids rubesanolides F (1) and G (2). Their structures were elucidated based on the analyses of the HRESIMS and 1D/2D NMR spectral data, and their absolute configurations were determined by ECD spectrum calculations and X-ray single crystal diffraction methods. Compounds 1 and 2, with a unique γ-lactone subgroup between C-8 and C-20, were found to form a carbonyl carbon at C-13 by removal of the isopropyl group in an abietane diterpene skeleton. Rubesanolide G (2) is a rare case of abietane that possesses a cis-fused configuration between rings B and C. The two isolates were evaluated for their biological activities against two cancer cell lines (A549 and HL60), three fungal strains (Candida alba, Aspergillus niger and Rhizopus nigricans) and three bacterial strains (Escherichia coli, Staphylococcus aureus and Bacillus subtilis).

C₂₈H₄₁NO₂, orthorhombic, P2₁2₁2₁ (no. 19), a = 10.4480(5) Å, b = 12.4188(6) Å, c = 18.3905(10) Å, V = 2386.2(2) ų, Z = 4, R _gt (F) = 0.0608, wR _ref (F ²) = 0.1510, T = 273(2) K.

C₂₈H₄₁NO, triclinic, P 1 $P1$ (no. 1), a = 6.3144(8) Å, b = 9.7315(13) Å, c = 10.5867(14) Å, α = 111.561(4)°, β = 96.486 ( 4 ) ∘ $96.486{(4)}^{\circ }$ , γ = 97.128(4)°, V = 591.41(14) ų, Z = 1, R g t ${R}_{\mathit{g}\mathit{t}}$ (F) = 0.0579, w R r e f $w{R}_{\mathit{r}\mathit{e}\mathit{f}}$ (F ²) = 0.1687, T = 273(2) K.