Cancer remains one of the leading causes of mortality worldwide, making early detection a critical factor in improving patient outcomes and survival rates. Developing advanced biosensors is essential for achieving early detection and accurate cancer diagnosis. This review offers a comprehensive overview of the development and application of carbon dots (CDs) and glassy carbon (GC) biosensors for early cancer detection. It covers the synthesis of CDs and GC, electrode fabrication methods, and electrochemical and optical transduction principles. This review explores various biosensors, including enzymatic and non-enzymatic, and discusses key biomarkers relevant to cancer detection. It also examines characterization techniques for electrochemical and optical biosensors, such as electrochemical impedance spectroscopy, cyclic voltammetry, UV–VIS, and confocal microscopy. The findings highlight the advancements in biosensor performance, emphasizing improvements in sensitivity, selectivity, and stability, as well as underscoring the potential of integrating different transduction methods and characterization approaches to enhance early cancer detection.

Cancer remains one of the leading causes of mortality worldwide, making early detection a critical factor in improving patient outcomes and survival rates. Developing advanced biosensors is essential for achieving early detection and accurate cancer diagnosis. This review offers a comprehensive overview of the development and application of carbon dots (CDs) and glassy carbon (GC) biosensors for early cancer detection. It covers the synthesis of CDs and GC, electrode fabrication methods, and electrochemical and optical transduction principles. The review explores various biosensors, including enzymatic and non-enzymatic, and discusses key biomarkers relevant to cancer detection. It also examines characterization techniques for electrochemical and optical biosensors, such as electrochemical impedance spectroscopy, cyclic voltammetry, UV-vis, and confocal microscopy. The findings highlight the advancements in biosensor performance, emphasizing improvements in sensitivity, selectivity, and stability, and underscore the potential of integrating different transduction methods and characterization approaches to enhance early cancer detection.