Objective: This study aimed to establish a dominant and non-dominant limb muscle strength evaluation model to evaluate the asymmetry of lower extremity muscle strength. Methods: The hip, knee, ankle flexors and extensors of 86 runners were evaluated separately in different contraction modes and at different movement speeds. A principal component analysis was used to establish a model for evaluating dominant and non-dominant lower extremity muscle strength and to comprehensively evaluate the asymmetry of lower extremity muscle strength. Results: Six main factors were present in both dominant and non-dominant indicators of lower extremity muscle strength, with dominant indicators of lower extremity muscle strength explaining 80.413% of the total variance and non-dominant indicators explaining 78.607% of the total variance. Conclusions: In a population of healthy male runners, there were differences in the symmetry of lower limbs in the comprehensive assessment model. The main contribution of the non-dominant side was the knee muscles, and the dominant side was the hip and knee muscles, so this difference should be considered in constructing future muscle strength evaluation models. It is critical to understanding the design and function of the human muscle system, and can reduce the number of meaningful tests we perform on diverse populations and help us reduce asymmetry.