Background/Objectives: Carvacrol, a monoterpenoid phenol found in essential oils, exhibits many biological activities, including anticancer properties through mechanisms such as induction of apoptosis. These properties can be enhanced if encapsulated within nanoparticles. This study focuses on producing functionalized carvacrol-loaded nanostructured lipid carriers (NLCs) applied to the treatment of breast cancer. Methods: NLCs were produced by hot emulsification with the sonication method and optimized by the Box–Behnken design, considering Precirol^® (1, 4, 7%), carvacrol (1, 5, 9%), and Tween^® (0.1, 0.5, 0.9%) as independent variables. Results: The optimized NLC containing 2% carvacrol had a particle size of 111 ± 2 nm, PdI of 0.26 ± 0.01, and zeta potential of −24 ± 0.8 mV. The solid lipid (Precirol^®) was the variable that most influenced particle size. NLCs were functionalized with Pluronic^® F68, cholesterol, chitosan, and polyethylene glycol (0.05–0.2%), with oNLC-Chol presenting the most promising results, with no significant increase in particle size (±12 nm) and high encapsulation efficiency (98%). Infrared spectra confirm effective carvacrol encapsulation, and stability tests showed no significant physicochemical changes for 120 days of storage at 4 °C. When incubated with albumin (5 mg/mL), NLCs showed overall good stability over 24 h, except for oNLC-Chol, which increased slightly in size after 24 h. In addition, oNLC increased the cytotoxic effect of carvacrol by 12-fold, resulting in an IC₅₀ of 7 ± 1 μg/mL. Conclusions: Therefore, it was possible to produce stable, homogeneous NLCs with nanometric sizes containing 2% carvacrol that displayed improved anticancer efficacy, indicating their potential as a delivery system.

: Despite their hazardous nature, snake venoms hold immense potential for the development of novel therapies. This summary delves into the key aspects of snake venom research, focusing on their significance as targets for neutralization, their utility as novel drugs, the application of in silico studies, and future prospects with nanotechnology. Significance of Snake Venom: Snake venom harbors a rich diversity of toxic proteins with a wide range of biological activities. Its importance lies in the possibility of neutralizing its detrimental effects and exploring its therapeutic potential for diverse ailments. Venom Neutralization: The development of more effective and specific antivenoms is crucial for treating snakebites, particularly in regions with a high prevalence of accidents. Molecular-level venom studies are essential for identifying novel targets for the development of more efficacious antivenoms. Venom as a Source of Novel Drugs: Proteins present in snake venom exhibit diverse pharmacological activities, including antithrombotic, anti-inflammatory, analgesic, and antimicrobial properties. Investigating these proteins can lead to the development of novel medications for various diseases. In silico Studies: Bioinformatics tools and molecular modelling can aid in the discovery of novel molecular targets in snake venom, accelerating the process of developing new drugs and therapies. Nanotechnology for Drug Delivery: Nanotechnology offers new possibilities for developing more efficient and targeted drug delivery systems, enhancing the safety and effectiveness of snake venom-based treatments. : Snake venom research represents a promising area of inquiry with immense potential for the development of novel drugs and therapies. The integration of traditional and innovative techniques, such as in silico studies and nanotechnology, can accelerate this process and contribute to the advancement of public health.

Poly(ε-caprolactone) nanocapsules containing breu branco ( Protium heptaphyllum ) essential oil were successfully prepared by nanoprecipitation and optimized using a Box–Behnken design. Spherical nanosystems were obtained, with a mean particle size of 172.10 ± 0.90 nm, a polydispersity index of 0.14 ± 0.04, a zeta potential of −25.49 ± 1.08 mV and an encapsulation efficiency above 99%, in addition to long-term colloidal stability. Cell viability assays in HaCaT cells showed high biocompatibility, with cell viability above 70% up to 6 mg/mL and only moderate toxicity at 12 mg/mL. In antimicrobial assays, the free oil had no inhibitory effect against Staphylococcus aureus , while the nanocapsules exhibited a minimum inhibitory concentration of 0.55 mg/mL, indicating enhanced antibacterial activity. Wound-healing assays further demonstrated improved keratinocyte migration compared to serum-free controls. Additionally, cytokine profiling by cytometric bead array indicated an anti-inflammatory profile, with strong suppression of IL-6 and selective increases in IL-2, IL-4, and TNF-α at higher doses (6 mg/mL) in nanocapsule-treated fibroblasts. Overall, these findings demonstrate that nanoencapsulation not only stabilizes breu branco essential oil but also enhances its antimicrobial, regenerative, and immunomodulatory effects, while fostering the sustainable valorization of Amazonian biodiversity.