The environment pollution with hydrophobic hydrocarbons is a serious problem that requires development of efficient strategies that would lead to bioremediation of contaminated areas. One of the common methods used for enhancement of biodegradation of pollutants is the addition of biosurfactants. Several mechanisms have been postulated as responsible for hydrocarbons bioavailability enhancement with biosurfactants. They include solubilization and desorption of pollutants as well as modification of bacteria cell surface properties. The presented review contains a wide discussion of these mechanisms in the context of alteration of bioremediation efficiency with biosurfactants. It brings new light to such a complex and important issue.

The objective of this research was the evaluation of the effects of exogenous added surfactants on hydrocarbon biodegradation and on cell surface properties. Crude oil hydrocarbons are often difficult to remove from the environment because of their insolubility in water. The addition of surfactants enhances the removal of hydrocarbons by raising the solubility of these compounds. These surfactants cause them to become more vulnerable to degradation, thereby facilitating transportation across the cell membrane. The obtained results showed that the microorganism consortia of bacteria are useful biological agents within environmental bioremediation. The most effective amongst all, as regards biodegradation, were the consortia of Pseudomonas spp. and Bacillus spp. strains. The results indicated that the natural surfactants (rhamnolipides and saponins) are more effective surfactants in hydrocarbon biodegradation as compared to Triton X-100. The addition of natural surfactants enhanced the removal of hydrocarbon and diesel oil from the environment. Very promising was the use of saponins as a surfactant in hydrocarbon biodegradation. This surfactant significantly increases the organic compound biodegradation. In the case of those surfactants that could be easily adsorbed on cells of strains (e.g., rhamnolipides), a change of hydrophobicity to ca. 30–40% was noted. As the final result, an increase in hydrocarbon biodegradation was observed.

Polycyclic aromatic hydrocarbons (PAH) contained in creosote oil are particularly difficult to remove from the soil environment. Their hydrophobic character and low bioavailability to soil microorganisms affects their rate of biodegradation. This study was performed on samples of soil that were (for over forty years) subjected to contamination with creosote oil, and their metagenome and physicochemical properties were characterized. Moreover, the study was undertaken to evaluate the biodegradation of PAHs by autochthonous consortia as well as by selected bacteria strains isolated from long-term contaminated industrial soil. From among the isolated microorganisms, the most effective in biodegrading the contaminants were the strains Pseudomonas mendocina and Brevundimonas olei. They were able to degrade more than 60% of the total content of PAHs during a 28-day test. The biodegradation of these compounds using AT7 dispersant was enhanced only by Serratia marcescens strain. Moreover, the addition of AT7 improved the effectiveness of fluorene and acenaphthene biodegradation by Serratia marcescens 6-fold. Our results indicated that long-term contact with aromatic compounds induced the bacterial strains to use the PAHs as a source of carbon and energy. We observed that supplementation with surfactants does not increase the efficiency of hydrocarbon biodegradation.