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The influence of daily prolonged administration of silver nanoparticles on the cognitive functions of a model mammal was studied. The accumulation of silver in the whole brain and the hippocampus, cerebellum, cortex and residual brain tissue of the mouse was investigated by highly precise and representative neutron activation analysis, and histological studies were conducted. Here, we show that long-term memory impairments were caused by the accumulation of silver nanoparticles in the brain and its subregions, such as the hippocampus, cerebellum and cortex, in a step-like manner by disturbance of hippocampal cell integrity. Three different approaches allowed us to observe this phenomenon and discover the reasons it occurred.
Anna Antsiferova; Marina Kopaeva; Vyacheslav Kochkin; Pavel Kashkarov; Mikhail Kovalchuk. Disturbance in Mammalian Cognition Caused by Accumulation of Silver in Brain. Toxics 2021, 9, 30 .
AMA StyleAnna Antsiferova, Marina Kopaeva, Vyacheslav Kochkin, Pavel Kashkarov, Mikhail Kovalchuk. Disturbance in Mammalian Cognition Caused by Accumulation of Silver in Brain. Toxics. 2021; 9 (2):30.
Chicago/Turabian StyleAnna Antsiferova; Marina Kopaeva; Vyacheslav Kochkin; Pavel Kashkarov; Mikhail Kovalchuk. 2021. "Disturbance in Mammalian Cognition Caused by Accumulation of Silver in Brain." Toxics 9, no. 2: 30.
We studied the effect of human lactoferrin (hLf) on degenerative changes in the nigrostriatal system and associated behavioral deficits in the animal model of Parkinson disease. Nigrostriatal dopaminergic injury was induced by single administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP; 40 mg/kg) to five-month-old C57Bl/6 mice. Behavioral disturbances were assessed in the open field and rotarod tests and by the stride length analysis. Structural deficits were assessed by the counts of tyrosine hydroxylase (TH)-immunoreactive neurons in the substantia nigra and optical density (OD) of TH-immunolabeled fibers in the striatum. Acute MPTP treatment induced long-term behavioral deficit and degenerative changes in the nigrostriatal system. Pretreatment with hLf prevented body weight loss and promoted recovery of motor functions and exploratory behavior. Importantly, OD of TH-positive fibers in the striatum of mice treated with hLf almost returned to normal, and the number of TH-positive cells in the substantia nigra significantly increased on day 28. These results indicate that hLf produces a neuroprotective effect and probably stimulates neuroregeneration under conditions of MPTP toxicity in our model. A relationship between behavioral deficits and nigrostriatal system disturbances at delayed terms after MPTP administration was found.
Marina Yu. Kopaeva; Anton B. Cherepov; Mikhail V. Nesterenko; Irina Yu. Zarayskaya. Pretreatment with Human Lactoferrin Had a Positive Effect on the Dynamics of Mouse Nigrostriatal System Recovery after Acute MPTP Exposure. Biology 2021, 10, 24 .
AMA StyleMarina Yu. Kopaeva, Anton B. Cherepov, Mikhail V. Nesterenko, Irina Yu. Zarayskaya. Pretreatment with Human Lactoferrin Had a Positive Effect on the Dynamics of Mouse Nigrostriatal System Recovery after Acute MPTP Exposure. Biology. 2021; 10 (1):24.
Chicago/Turabian StyleMarina Yu. Kopaeva; Anton B. Cherepov; Mikhail V. Nesterenko; Irina Yu. Zarayskaya. 2021. "Pretreatment with Human Lactoferrin Had a Positive Effect on the Dynamics of Mouse Nigrostriatal System Recovery after Acute MPTP Exposure." Biology 10, no. 1: 24.
Целью настоящей работы стало исследование эффектов лактоферрина (Лф) человека у мышей после острого гамма-облучения в сублетальной дозе. Методы. Исследование было проведено на 2-2,5-месячных самцах мышей линии C57Bl/6. Животные из экспериментальных групп были подвергнуты общему воздействию гамма-излучения в дозе 7,5 Гр. Сразу после облучения и повторно через 24 часа после него часть животных получила инъекцию Лф (внутрибрюшинно, 4 мг на животное). Было изучено влияние Лф на выживаемость и среднюю продолжительность жизни мышей. Для оценки общей двигательной и исследовательской активности использовали тест «Открытое поле». Результаты. Введение Лф позволило увеличить выживаемость и среднюю продолжительность жизни облученных мышей в течение эксперимента. Происходила более быстрая нормализация динамики изменения массы тела. Кроме того, Лф оказал компенсаторное действие на исследовательскую активность облученных животных. The aim of this study was to investigate effects of human lactoferrin (Lf) in mice exposed to acute gamma-irradiation at a sublethal dose. Methods. C57Вl/6 2-2.5-month-old male mice were used for the experiments. Animals from experimental groups were exposed to whole-body gamma-radiation at a dose of 7.5 Gy. Some animals received an intraperitoneal injection of Lf (4 mg per animal) immediately and then at 24 hours after the irradiation. The effect of Lf on survival rate and life span was studied. The open field test was used to assess locomotor and research activity. Results. The Lf administration increased the survival rate and life span of irradiated mice during the experiment. The dynamics of body weight normalized faster. In addition, Lf exerted a compensatory effect on the research activity of irradiated animals.
Marina Kopaeva; I. B. Alchinova; M. V. Nesterenko; A. B. Cherepov; И.ю. Зарайская; M. Yu. Karganov. Lactoferrin beneficially influences the recovery of physiological and behavioral indexes in mice exposed to acute gamma-irradiation. Nauchno-prakticheskii zhurnal «Patogenez» 2020, 18, 29 -33.
AMA StyleMarina Kopaeva, I. B. Alchinova, M. V. Nesterenko, A. B. Cherepov, И.ю. Зарайская, M. Yu. Karganov. Lactoferrin beneficially influences the recovery of physiological and behavioral indexes in mice exposed to acute gamma-irradiation. Nauchno-prakticheskii zhurnal «Patogenez». 2020; 18 (1):29-33.
Chicago/Turabian StyleMarina Kopaeva; I. B. Alchinova; M. V. Nesterenko; A. B. Cherepov; И.ю. Зарайская; M. Yu. Karganov. 2020. "Lactoferrin beneficially influences the recovery of physiological and behavioral indexes in mice exposed to acute gamma-irradiation." Nauchno-prakticheskii zhurnal «Patogenez» 18, no. 1: 29-33.
We studied different ways of transport of human lactoferrin to the brain of C57Bl/6 mice after its administration via different routes, analyzed its distribution in the brain, and determined the phenotype of lactoferrin-containing cells. Colocalization of lactoferrin and markers of various cell types was estimated by fluorescent immunohistochemical analysis. Lactoferrin was detected in mouse brain sections after its intranasal, sublingual, and intraperitoneal administration, but not after conjunctival administration. After intranasal administration, lactoferrin rapidly penetrated into the brain and accumulated in the cytoplasm of vascular endothelial cells in the neocortex, striatum, hippocampus, and thalamus. After application of protein solution onto fixed floating sections, highly specific binding of lactoferrin was found in the nuclei of neurons, astrocytes, and microglia cells, but not in the nuclei of endothelial cells of mouse brain.
Marina Kopaeva; Anton Cherepov; I. Yu. Zarayskaya; M. V. Nesterenko. Transport of Human Lactoferrin into Mouse Brain: Administration Routes and Distribution. Bulletin of Experimental Biology and Medicine 2019, 167, 561 -567.
AMA StyleMarina Kopaeva, Anton Cherepov, I. Yu. Zarayskaya, M. V. Nesterenko. Transport of Human Lactoferrin into Mouse Brain: Administration Routes and Distribution. Bulletin of Experimental Biology and Medicine. 2019; 167 (4):561-567.
Chicago/Turabian StyleMarina Kopaeva; Anton Cherepov; I. Yu. Zarayskaya; M. V. Nesterenko. 2019. "Transport of Human Lactoferrin into Mouse Brain: Administration Routes and Distribution." Bulletin of Experimental Biology and Medicine 167, no. 4: 561-567.
Marina Kopaeva; Anton Cherepov; Irina Zarayskaya; Mikhail Nesterenko. THE USE OF DIFFERENT METHODS OF HUMAN LACTOFERRIN ADMINISTRATION TO MICE FOLLOWED BY ITS LOCALIZATION IN THE BRAIN. Neuroscience for Medicine and Psychology 2019, 1 .
AMA StyleMarina Kopaeva, Anton Cherepov, Irina Zarayskaya, Mikhail Nesterenko. THE USE OF DIFFERENT METHODS OF HUMAN LACTOFERRIN ADMINISTRATION TO MICE FOLLOWED BY ITS LOCALIZATION IN THE BRAIN. Neuroscience for Medicine and Psychology. 2019; ():1.
Chicago/Turabian StyleMarina Kopaeva; Anton Cherepov; Irina Zarayskaya; Mikhail Nesterenko. 2019. "THE USE OF DIFFERENT METHODS OF HUMAN LACTOFERRIN ADMINISTRATION TO MICE FOLLOWED BY ITS LOCALIZATION IN THE BRAIN." Neuroscience for Medicine and Psychology , no. : 1.
A.V. Rodina; Yu.P. Semochkina; Marina Kopaeva; A.N. Strepetov; V.V. Safronov; E.Yu. Moskaleva; Federal Scientific Research Centre “Crystallography and Photonics” Russian Academy of Sciences; National Research Center «Kurchatov Institute». STATE OF MICROGLIA AND COGNITIVE FUNCTIONS IN MICE IN THE DELAYED PERIOD AFTER COMBINED EXPOSURE TO LOW DOSES OF GAMMA- AND GAMMA-NEUTRON RADIATION. Aerospace and Environmental Medicine 2019, 53, 1 .
AMA StyleA.V. Rodina, Yu.P. Semochkina, Marina Kopaeva, A.N. Strepetov, V.V. Safronov, E.Yu. Moskaleva, Federal Scientific Research Centre “Crystallography and Photonics” Russian Academy of Sciences, National Research Center «Kurchatov Institute». STATE OF MICROGLIA AND COGNITIVE FUNCTIONS IN MICE IN THE DELAYED PERIOD AFTER COMBINED EXPOSURE TO LOW DOSES OF GAMMA- AND GAMMA-NEUTRON RADIATION. Aerospace and Environmental Medicine. 2019; 53 (4):1.
Chicago/Turabian StyleA.V. Rodina; Yu.P. Semochkina; Marina Kopaeva; A.N. Strepetov; V.V. Safronov; E.Yu. Moskaleva; Federal Scientific Research Centre “Crystallography and Photonics” Russian Academy of Sciences; National Research Center «Kurchatov Institute». 2019. "STATE OF MICROGLIA AND COGNITIVE FUNCTIONS IN MICE IN THE DELAYED PERIOD AFTER COMBINED EXPOSURE TO LOW DOSES OF GAMMA- AND GAMMA-NEUTRON RADIATION." Aerospace and Environmental Medicine 53, no. 4: 1.
Anna A. Antsiferova; Marina Kopaeva; Vyacheslav N. Kochkin; Pavel K. Kashkarov; Mikhail V. Kovalchuk. Accumulation of Silver Nanoparticles in Mice Brain Parts and the Harmful Effects. Journal of Nanomedicine & Nanotechnology 2019, 10, 1 -7.
AMA StyleAnna A. Antsiferova, Marina Kopaeva, Vyacheslav N. Kochkin, Pavel K. Kashkarov, Mikhail V. Kovalchuk. Accumulation of Silver Nanoparticles in Mice Brain Parts and the Harmful Effects. Journal of Nanomedicine & Nanotechnology. 2019; 10 (1):1-7.
Chicago/Turabian StyleAnna A. Antsiferova; Marina Kopaeva; Vyacheslav N. Kochkin; Pavel K. Kashkarov; Mikhail V. Kovalchuk. 2019. "Accumulation of Silver Nanoparticles in Mice Brain Parts and the Harmful Effects." Journal of Nanomedicine & Nanotechnology 10, no. 1: 1-7.
Silver nanoparticles have been widely used in the lighting and food industries, in medicine, and in pharmaceutics as an antiseptic agent. Recent research demonstrates that, after prolonged oral administration, silver nanoparticles may cross the blood-brain barrier and accumulate in the brain in rather high amounts. In ex vivo experiments, it has also been shown that silver nanoparticles demonstrate neurotoxicity. The objective of this work was to answer the questions whether silver nanoparticles change cognitive and behavioral functions of mammals after prolonged administration if silver nanoparticles have accumulated in the brain. C57Bl/6 male mice were orally exposed to PVP-coated silver nanoparticles daily for 30, 60, 120 and 180 days. Control mice were exposed to distilled water. After that they were tested in the Open Field, Elevated Plus Maze, Light-Dark Box and contextual fear conditioning task. The data have shown that the experimental mice went through three periods of switching in the behavior caused by adaptation to the toxic silver nanoparticles: anxiety, appearance of research instinct and impairment of long-term memory. This provides evidence of the hazardous effect of silver nanoparticles, which appears after long periods of silver nanoparticle oral administration.
Anna Antsiferova; Marina Kopaeva; Pavel Kashkarov. Effects of Prolonged Silver Nanoparticle Exposure on the Contextual Cognition and Behavior of Mammals. Materials 2018, 11, 558 .
AMA StyleAnna Antsiferova, Marina Kopaeva, Pavel Kashkarov. Effects of Prolonged Silver Nanoparticle Exposure on the Contextual Cognition and Behavior of Mammals. Materials. 2018; 11 (4):558.
Chicago/Turabian StyleAnna Antsiferova; Marina Kopaeva; Pavel Kashkarov. 2018. "Effects of Prolonged Silver Nanoparticle Exposure on the Contextual Cognition and Behavior of Mammals." Materials 11, no. 4: 558.