Biofilm-related infections play a significant role in the development and persistence of ventilator-associated pneumonia. Pseudomonas aeruginosa (P. aeruginosa) frequently causes biofilm-related infections associated with ventilator tubing. Glucosamine gold nanoparticles (AuNPs) may exhibit antibiofilm properties; however, more studies, including combinatorial therapy with antibiotics, are needed to explore their potential applications in clinical settings. This study aims to investigate the biofilm inhibition properties of glucosamine AuNPs in combination with meropenem against P. aeruginosa ATCC 9027 on the endotracheal tube. A biofilm inhibition assay of glucosamine AuNPs at 0.02 mg/mL, both singly and in combination with meropenem at 1 mg/mL, was carried out against P. aeruginosa ATCC 9027 on an endotracheal tube using the tissue culture plate method. Scanning electron microscopy was performed for visualization. Glucosamine AuNPs at 0.02 mg/mL combined with meropenem at 1 mg/mL showed greater biofilm inhibition (72%) on the endotracheal tube than glucosamine nanoparticles at 0.02 mg/mL alone (26%) (p = 0.001). The scanning electron microscopic visualization revealed that the untreated P. aeruginosa biofilm was denser than the glucosamine nanoparticles-treated biofilm, whether combined with meropenem or using glucosamine nanoparticles alone. The combination of glucosamine AuNPs and meropenem may have the synergistic effect of inhibiting biofilm production of P. aeruginosa on the endotracheal tubes of patients with mechanical ventilation. Conducting additional experiments to explore the impact of combining glucosamine-coated gold nanoparticles (AuNPs) with meropenem on the inhibition of biofilm production by clinical P. aeruginosa isolates would be beneficial.

Stunting merupakan masalah kesehatan dengan efek jangka panjang berupa risiko penurunan produktivitas pembangunan nasional. Dampak stunting dapat dicegah dengan perbaikan literasi anak, guru dan orang tua saat anak usia sekolah dasar. Disbiosis saluran cerna sebagai salah satu faktor yang berperan pada kejadian stunting dapat dicegah dengan pola makan seimbang serta pola hidup bersih dan sehat. Desain program pengabdian masyarakat ini bersifat partisipatoris dengan melibatkan guru, siswa, dan orang tua terkait literasi tentang stunting. Program dilakukan dalam beberapa tahap meliputi penyusunan agenda bersama, penyuluhan edukasi, pemilihan duta siswa sehat, serta diskusi penyusunan rencana pengembangan program dengan guru. Dari pengabdian masyarakat yang dilakukan, didapatkan perubahan literasi kognitif siswa terhadap perilaku sehat yang berhubungan dengan stunting seperti pola makan seimbang dan pola hidup bersih dan sehat. Sementara diskusi dengan guru memunculkan upaya kerjasama penguatan program UKS (Usaha Kesehatan Sekolah) dengan pola UES (University to Elementary School) sebagai bentuk tindak lanjut untuk melembagakan upaya peningkatan kesehatan siswa secara konstruktif. Penelitian lebih lanjut perlu dilakukan sebagai evaluasi jangka panjang dari pengabdian, terutama pada dampak lanjut dari duta siswa dan penguatan UKS melalui skema UES.

Biofilm-related infections play a significant role in the development and persistence of ventilator-associated pneumonia. Pseudomonas aeruginosa frequently causes biofilm-related infections associated with ventilator tubing. Glucosamine gold nanoparticles (AuNPs) may exhibit antibiofilm properties; however, more studies, including combinatorial therapy with antibiotics, are needed to explore their potential applications in clinical settings. This study aimed to investigate the biofilm inhibition properties of glucosamine AuNPs in combination with meropenem against Pseudomonas aeruginosa ATCC 9027 on the endotracheal tube. A biofilm inhibition assay of glucosamine AuNPs at 0.02 mg/ml, both singly and in combination with meropenem at 1 mg/ml, was carried out against Pseudomonas aeruginosa ATCC 9027 on an endotracheal tube using the tissue culture plate method. Scanning electron microscopy was performed for visualization. Glucosamine AuNPs at 0.02 mg/ml combined with meropenem at 1 mg/ml showed greater biofilm inhibition (72%) than glucosamine nanoparticles at 0.02 mg/ml alone (26%) on the endotracheal tube (p=0.001). The scanning electron microscopic visualization revealed that the untreated Pseudomonas aeruginosa biofilm was denser than the glucosamine nanoparticles-treated biofilm either combined with meropenem or glucosamine nanoparticles alone. The combination of glucosamine AuNPs and meropenem may have the synergistic effect to inhibit biofilm production of Pseudomonas aeruginosa on the endotracheal tube in patient with mechanical ventilation. Conducting additional experiments to explore the impact of combining glucosamine-coated gold nanoparticles (AuNPs) with meropenem on the inhibition of biofilm production by clinical Pseudomonas aeruginosa isolates would be beneficial.