Infertility affects approximately one-sixth of couples globally, with the incidence of male infertility steadily increasing. However, our understanding of the impact of viral infections on fertility remains limited. This review consolidates findings from previous studies, outlining 40 viruses identified in human semen and summarizing their key characteristics, modes of transmission, and their effects on both the reproductive and endocrine systems. Furthermore, it elucidates potential pathogenic mechanisms and treatment prospects of viruses strongly associated with male infertility. This synthesis will enhance our comprehension of how viral infections influence male reproductive health, offering valuable insights for future research as well as the diagnosis and treatment of infectious infertility. Abbreviated summary: Semen viral infections can damage the male reproductive system, further affecting semen quality and causing infertility, as well as sexually transmitted infections to partners and vertical transmission resulting in poor pregnancy outcomes.

Precise gene regulation is critical during embryo development. Long terminal repeat elements (LTRs) of endogenous retroviruses (ERVs) are dynamically expressed in blastocysts of mammalian embryos. However, the expression pattern of LTRs in monkey blastocyst is still unknown. By single-cell RNA-sequencing (seq) data of cynomolgus monkeys, we found that LTRs of several ERV families, including MacERV6, MacERV3, MacERV2, MacERVK1, and MacERVK2, were highly expressed in pre-implantation embryo cells including epiblast (EPI), trophectoderm (TrB), and primitive endoderm (PrE), but were depleted in post-implantation. We knocked down MacERV6-LTR1a in cynomolgus monkeys with a short hairpin RNA (shRNA) strategy to examine the potential function of MacERV6-LTR1a in the early development of monkey embryos. The silence of MacERV6-LTR1a mainly postpones the differentiation of TrB, EPI, and PrE cells in embryos at day 7 compared to control. Moreover, we confirmed MacERV6-LTR1a could recruit Estrogen Related Receptor Beta (ESRRB), which plays an important role in the maintenance of self-renewal and pluripotency of embryonic and trophoblast stem cells through different signaling pathways including FGF and Wnt signaling pathways. In summary, these results suggest that MacERV6-LTR1a is involved in gene regulation of the pre-implantation embryo of the cynomolgus monkeys.

Background: Laser-assisted hatching (LAH) stands as the predominant technique for removing the zona pellucida (ZP) in embryos, primarily consisting of two methods: drilling laser-assisted hatching (D-LAH) and thinning laser-assisted hatching (T-LAH). Presently, both methods have limitations, and their comparative efficacy for embryo implantation and clinical pregnancy remains uncertain. Aim: Evaluate the impact of D-LAH and T-LAH on clinical pregnancy rates within assisted reproductive technology (ART). Methods: We systematically searched electronic databases including PubMed, Web of Science, and Cochrane Library until July 20, 2022. This study encompassed observational studies and randomized controlled trials (RCTs). A 95% confidence interval (CI) was utilized for assessing the risk ratio (RR) of pregnancy outcomes. The level of heterogeneity was measured using I² statistics, considering a value exceeding 50% as indicative of substantial heterogeneity. Results: The meta-analysis scrutinized 9 studies involving 2405 clinical pregnancies from D-LAH and 2239 from T-LAH. Findings suggested no considerable variation in the clinical pregnancy rates between the two techniques (RR = 0.93, 95% CI: 0.79–1.10, I² = 71%, P = 0.41). Subgroup analyses also revealed no substantial differences. However, D-LAH exhibited a notably higher occurrence of singleton pregnancies compared to T-LAH (RR = 2.28, 95% CI: 1.08–4.82, I² = 89%, P = 0.03). There were no noteworthy distinctions observed in other secondary outcomes encompassing implantation rate, multiple pregnancies, ongoing pregnancy, miscarriage, premature birth, and live birth. Conclusion: Both the primary findings and subgroup analyses showed no marked variance in clinical pregnancy rates between D-LAH and T-LAH. Therefore, patients with varying conditions should select their preferred LAH technique after assessing their individual situation. However, due to the restricted number of studies involved, accurately gauging the influence of these laser techniques on clinical outcomes is challenging, necessitating further RCTs and high-quality studies to enhance the success rate of ART. Trial registration: PROSPERO: CRD42022347066.

Background: Infertility is a prevalent global reproductive health issue. In vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI), as pivotal assisted reproductive technologies, are widely implemented in clinical practice. However, clinical pregnancy outcomes following IVF/ICSI are influenced by various factors, making accurate prediction essential for optimizing treatment strategies. Objective: To develop and validate a predictive model for clinical pregnancy outcomes following IVF/ICSI treatment. Methods: A retrospective analysis was conducted on clinical data from 154,307 patients who underwent assisted reproductive treatment due to infertility at the First People’s Hospital of Yunnan Province. Based on inclusion and exclusion criteria, 11,449 patients who underwent IVF/ICSI were included. Key predictors were identified using LASSO regression. A Nomogram scoring system was developed for an intuitive visualization of individualized prediction results. Model performance was evaluated using the area under the receiver operating characteristic (ROC) curve, calibration curves, decision curve analysis (DCA), and clinical impact curves. Results: LASSO regression identified eight critical predictors influencing clinical pregnancy outcomes: male age, antral follicle count (AFC), Day 3 follicle-stimulating hormone (FSH) level, endometrial thickness, female age, number of usable embryos, number of high-quality blastocysts, and number of embryos transferred. The predictive model demonstrated excellent performance in both the training and validation cohorts, with AUC values of 0.839 [95% CI (0.825, 0.852)] and 0.827 [95% CI (0.817, 0.835)], respectively, indicating strong discriminatory ability. Calibration curves confirmed a high degree of consistency between predicted probabilities and actual outcomes. Decision curve analysis revealed substantial net clinical benefit across various risk thresholds, while clinical impact curves further validated the model’s practical applicability in clinical settings. Conclusion: This study identified key factors influencing clinical pregnancy outcomes following IVF/ICSI treatment, including male age, antral follicle count (AFC), Day 3 follicle-stimulating hormone (FSH) level, endometrial thickness, female age, number of usable embryos, number of high-quality blastocysts, and number of embryos transferred. This model serves as a scientifically sound decision-support tool for clinicians in the management of infertility treatment with IVF/ICSI.

The recurrence of latent toxoplasmosis is a frequent cause of toxoplasmic encephalopathy in individuals with immunosuppressive conditions or medication. If left untreated or diagnosed is delayed, it often resulting in fatality. The pathogen of Toxoplasma gondii has been identified in cerebrospinal fluid cytology and a limited number of documented cases of cerebral toxoplasmosis, typically observed in those with acquired immunodeficiency syndrome (AIDS). We report the identification of Toxoplasma gondii tachyzoites in the cerebrospinal fluid of a patient diagnosed with systemic lupus erythematosus (SLE) and concurrent SARS-CoV-2 positive infection. The presented case suggests that cerebral toxoplasmosis should be promptly considered when patients with systemic lupus erythematosus exhibit neurological manifestations.