Mn₃O₄ nanoparticles have been synthesized using alcoholic extracts, at pH = 7 and low temperature (60 °C), from different masses (1.00, 3.00, 5.00, and 7.00 g) of fresh leaves from Nerium oleander and Bixa orellana, without additional heat treatment. Appropriate techniques were used to identify the secondary metabolites of the extracts and evaluate the structural, optical, and chemical properties of the nanoparticles. The XRD results confirmed the formation of Mn₃O₄ nanoparticles with crystallite size in the 5−8 nm range, with more notable effects on the crystallinity of the nanoparticles obtained with B. orellana extracts. The greatest effect on the bandgap was observed in nanoparticles synthesized with N. oleander extracts. Raman spectra confirmed phonon confinement, and in the PL spectra, emission bands associated with structural defects, such as oxygen vacancies, were observed. In FTIR spectra, the main bands of Mn₃O₄ were identified, whose intensity decreased as the concentration of extract and other bands associated with functional groups of the extract increased. TEM images showed nanoparticles were spherical with 7.81 nm (N1) and 7.94 nm (B1) average diameters. The extract from N. oleander leaves was more appropriate than that from B. orellana for the synthesis of Mn₃O₄ nanoparticles under the conditions used.

Entre los meses de agosto de 2015 a enero del 2016, se evaluaron 77 estudiantes voluntarios del Centro de Educación Superior Técnico de la Universidad Nacional de Trujillo (CESTUNT) previo consentimiento informado, con el objetivo de determinar la frecuencia fenotípica de grupos sanguíneos ABO y factor Rh (D), mediante el método en lámina, empleando anticuerpos monoclonales para la identificación del grupo sanguíneo ABO y factor Rh (D). Los resultados en relación con la distribución por carrera técnica y sexo de los estudiantes del CESTUNT, muestran que la mayor frecuencia fenotípica es para el grupo sanguíneo O y Factor Rh (D)⁺. PALABRAS CLAVE: grupo sanguíneo, factor Rh (D), sistema sanguíneo ABO, transfusión sanguínea. DOI: http://dx.doi.org/10.17268/rmt.2020.v15i02.04